Results 51 to 60 of about 5,306,282 (389)
Molecules, 2019 Capillary electrophoresis using laser-induced fluorescence detection (CE-LIF) is one of the most sensitive separation tools among electrical separation methods.
Binh Thanh Nguyen, Min-Jung Kang
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Journal of Natural Medicines, 2017 Immunoassays are antibody-based analytical methods for quantitative/qualitative analysis. Since the principle of immunoassays is based on specific antigen–antibody reaction, the assays have been utilized worldwide for diagnosis, pharmacokinetic studies ...
S. Sakamoto +6 more
semanticscholar +1 more source
Metabolites, 2018 The Kulka resorcinol assay (Kulka, R.G., Biochemistry 1956, 63, 542⁻548) for ketoses has been widely used in the literature but suffers from two major disadvantages: (a) it employs large amounts of potentially harmful reagents for a general biology
Shreya Shaw, Robin Ghosh
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Enzyme-linked immunospot: an alternative method for the detection of interferon gamma in Johne's disease [PDF]
, 2009 . To date, the sensitivity of the interferon gamma (IFN-c) enzyme-linked immunosorbent assay (ELISA) to detect Johne’s disease (JD) has been poor, especially in the early stages of disease.
Begg, Douglas J. +6 more
core +2 more sources
The Development of Assays for Heparanase Enzymatic Activity: Towards a Gold Standard
Molecules, 2018 The enzyme heparanase, an endo-β-glucuronidase, degrades heparan sulfate (HS) chains on the cell surface and in the extracellular matrix. Heparanase regulates numerous biological processes that drive tumour growth, metastasis and angiogenesis.
Mohit Chhabra, Vito Ferro
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Nature Biotechnology, 2010 The ability to detect single protein molecules in blood could accelerate the discovery and use of more sensitive diagnostic biomarkers. To detect low-abundance proteins in blood, we captured them on microscopic beads decorated with specific antibodies ...
David M. Rissin +14 more
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Deactivation of TEM-1 beta-Lactamase investigated by isothermal batch and non-isothermal continuous enzyme membrane reactor methods [PDF]
, 2009 The thermal deactivation of TEM-1 β-lactamase was examined using two experimental techniques: a series of isothermal batch assays and a single, continuous, non-isothermal assay in an enzyme membrane reactor (EMR).
Bommarius, Andreas S. +2 more
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Paper-Based Enzyme Competition Assay for Detecting Falsified β-Lactam Antibiotics.
ACS Sensors, 2018 Falsified and substandard antibiotics are a growing worldwide problem that leads to increased patient mortality and decreased trust in healthcare, and contributes to antimicrobial resistance.
K. Boehle +3 more
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ACS Nano, 2017 Enzyme-based colorimetric assays have been widely used in research laboratories and clinical diagnosis for decades. Nevertheless, as constrained by the performance of enzymes, their detection sensitivity has not been substantially improved in recent ...
H. Ye +7 more
semanticscholar +1 more source
Perspectives in Science, 2014 AbstractThe essential requirements for enzyme assays are described and frequently occurring errors and pitfalls as well as their avoidance are discussed. The main factors, which must be considered for assaying enzymes, are temperature, pH, ionic strength and the proper concentrations of the essential components like substrates and enzymes ...
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