Results 171 to 180 of about 651,887 (304)

Meta‐analysis fails to show any correlation between protein abundance and ubiquitination changes

FEBS Open Bio, EarlyView.
We analyzed over 50 published proteomics datasets to explore the relationship between protein levels and ubiquitination changes across multiple experimental conditions and biological systems. Although ubiquitination is often associated with protein degradation, our analysis shows that changes in ubiquitination do not globally correlate with changes in ...
Nerea Osinalde, Unai Alduntzin, Gorka Prieto, Ugo Mayor   +3 more
wiley   +1 more source

Enzyme Engineering Database (EnzEngDB): a platform for sharing and interpreting sequence-function relationships across protein engineering campaigns. [PDF]

Nucleic Acids Res
Long Y, Abbasinejad F, Li FZ, Reinprecht P, Wittmann B, Kennemur JL, Carder H, Yang J, Lambert T, O'Meara R, Radtke L, Qin Z, Brinkmann-Chen S, Arnold F, Mora A.   +14 more
europepmc   +1 more source

Therapeutic enzyme engineering using a generative neural network. [PDF]

Sci Rep, 2022
Giessel A, Dousis A, Ravichandran K, Smith K, Sur S, McFadyen I, Zheng W, Licht S.   +7 more
europepmc   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Physics-based modeling in the new era of enzyme engineering. [PDF]

Nat Comput Sci
Jurich C, Shao Q, Ran X, Yang ZJ.
europepmc   +1 more source

Enzyme engineering and in vivo testing of a formate reduction pathway. [PDF]

Synth Biol (Oxf), 2021
Wang J, Anderson K, Yang E, He L, Lidstrom ME.   +4 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Expanding the Repertoire of Photoswitchable Unnatural Amino Acids for Enzyme Engineering. [PDF]

Angew Chem Int Ed Engl
Hiefinger C, Marcon M, Pape V, Casadevall G, Lahmy R, Haag C, Nazet J, Bartl M, Bruckmann A, Osuna S, König B, Hupfeld A.   +11 more
europepmc   +1 more source

Machine learning for enzyme engineering, selection and design. [PDF]

Protein Eng Des Sel, 2021
Feehan R, Montezano D, Slusky JSG.
europepmc   +1 more source

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source