Results 161 to 170 of about 6,124,915 (260)

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

Computationally guided enzyme engineering for regioselective synthesis of fucosylated human milk oligosaccharides. [PDF]

Appl Microbiol Biotechnol
Yang Y, Vega A, Holck J, Planas A, Biarnés X, Zeuner B.   +5 more
europepmc   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Biocatalytic reduction of nitro-sulfonamides: enzyme engineering, process integration, and sustainable pathways to <i>p</i>-aminobenzenesulfonamide. [PDF]

Front Bioeng Biotechnol
Ai R, Muhammad N, Cui Y, Wang L, Wang Y, Weng S, Kakar MU, Khan S, Ahmad S.   +8 more
europepmc   +1 more source

Machine Learning-Supported Enzyme Engineering toward Improved CO₂-Fixation of Glycolyl-CoA Carboxylase. [PDF]

ACS Synth Biol, 2023
Marchal DG, Schulz L, Schuster I, Ivanovska J, Paczia N, Prinz S, Zarzycki J, Erb TJ.   +7 more
europepmc   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Synzymes: The Future of Modern Enzyme Engineering. [PDF]

Appl Biochem Biotechnol
Palabiyik AA.
europepmc   +1 more source

Ultrahigh-Throughput Enzyme Engineering and Discovery in <i>In Vitro</i> Compartments. [PDF]

Chem Rev, 2023
Gantz M, Neun S, Medcalf EJ, van Vliet LD, Hollfelder F.   +4 more
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Confocal Absorbance-Activated Droplet Sorting (cAADS) for Enzyme Engineering. [PDF]

Adv Sci (Weinh)
Kaba AM, Gounel S, Beneyton T, Buisson L, Baret JC, Mano N.   +5 more
europepmc   +1 more source