Results 161 to 170 of about 658,387 (301)

Data-driven enzyme engineering to identify function-enhancing enzymes. [PDF]

Protein Eng Des Sel, 2023
Jiang Y, Ran X, Yang ZJ.
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Protein modelling for enzyme engineering [PDF]

, 2017
Guallar, Victor, Santiago, Gerard
core  

Confocal Absorbance-Activated Droplet Sorting (cAADS) for Enzyme Engineering. [PDF]

Adv Sci (Weinh)
Kaba AM, Gounel S, Beneyton T, Buisson L, Baret JC, Mano N.   +5 more
europepmc   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Organelle-Targeted Multi-Enzyme Engineering Enables Medium-Chain Fatty Acid Production in <i>Chlamydomonas reinhardtii</i>. [PDF]

J Microbiol Biotechnol
Roh Y, Shin S, Han CH, Eom JI, Park S, Son M, Pan CH, Shin CH, Yamaoka Y.   +8 more
europepmc   +1 more source

Nature-inspired Enzyme engineering and sustainable catalysis: biochemical clues from the world of plants and extremophiles. [PDF]

Front Bioeng Biotechnol, 2023
Chatterjee A, Puri S, Sharma PK, Deepa PR, Chowdhury S.   +4 more
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Enzyme Engineering Database (EnzEngDB): a platform for sharing and interpreting sequence-function relationships across protein engineering campaigns. [PDF]

Nucleic Acids Res
Long Y, Abbasinejad F, Li FZ, Reinprecht P, Wittmann B, Kennemur JL, Carder H, Yang J, Lambert T, O'Meara R, Radtke L, Qin Z, Brinkmann-Chen S, Arnold F, Mora A.   +14 more
europepmc   +1 more source

Directed Evolution Methods for Enzyme Engineering. [PDF]

Molecules, 2021
Nirantar SR.
europepmc   +1 more source