Results 191 to 200 of about 651,887 (304)

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Efficient synthesis of l-DOPA in <i>Escherichia coli</i> via cofactor and enzyme engineering. [PDF]

Synth Syst Biotechnol
Deng L, Ping J, Liu Z, Linghu K, Zhang H, Shan X, Zeng W, Li J, Zhou J.   +8 more
europepmc   +1 more source

Applications of Synthetic Biotechnology on Carbon Neutrality Research: A Review on Electrically Driven Microbial and Enzyme Engineering. [PDF]

Front Bioeng Biotechnol, 2022
Zhuang X, Zhang Y, Xiao AF, Zhang A, Fang B.   +4 more
europepmc   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

Combination of enzyme engineering and quorum sensing system for efficient de novo biosynthesis of β-arbutin in <i>Escherichia coli</i>. [PDF]

Synth Syst Biotechnol
Liu Z, Liu D, Deng L, LingHu K, Shan X, Zhou J, Zeng W.   +6 more
europepmc   +1 more source

Evolving strategies for marine enzyme engineering: recent advances on the molecular modification of alginate lyase. [PDF]

Mar Life Sci Technol, 2022
Cao S, Li Q, Xu Y, Tang T, Ning L, Zhu B.   +5 more
europepmc   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Accelerated enzyme engineering by machine-learning guided cell-free expression. [PDF]

Nat Commun
Landwehr GM, Bogart JW, Magalhaes C, Hammarlund EG, Karim AS, Jewett MC.   +5 more
europepmc   +1 more source

Toward scalable biocatalytic conversion of 5-hydroxymethylfurfural by galactose oxidase using coordinated reaction and enzyme engineering. [PDF]

Nat Commun, 2021
Birmingham WR, Toftgaard Pedersen A, Dias Gomes M, Bøje Madsen M, Breuer M, Woodley JM, Turner NJ.   +6 more
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source