Results 81 to 90 of about 308,992 (299)

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Forecasting and dynamical modeling of reversible enzymatic reactions with a hybrid proportional fractional derivative

Frontiers in Physics
Chemical kinetics is a branch of chemistry that investigates the rates of chemical reactions and has applications in cosmology, geology, and physiology.
Parvaiz Ahmad Naik, Anum Zehra, Muhammad Farman, Muhammad Farman, Aamir Shehzad, Sundas Shahzeen, Zhengxin Huang   +6 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Asymptotic approximations for Mechaelis-Menten type

, 2007
The nature and validity of the mathematical formulation of Michaelis-Menten type kinetics for enzyme-catalysed biochemical reactions is studied. Almost all previous work has concentrated on isolated reactions, i.e.
Kinetics For Enzyme-Catalysed, I. Stoleriu, J. L. Liu   +2 more
core  

Effect of pH on the Hydrolytic Kinetics of Gamma-Glutamyl Transferase from Bacillus subtilis

The Scientific World Journal, 2014
The effect of pH on the steady state kinetics of gamma-glutamyl transferase (GGT) from Bacillus subtilis was examined using glutamyl-(3-carboxyl)-4-nitroanilide as the chromogenic reporter substrate.
Sharath Balakrishna, Asmita Prabhune
doaj   +1 more source

Kinetics of Hydrolyzing Isolated Soy Protein by an Endopeptidase and its Conceptual Application in Process Engineering

International Journal of Food Studies, 2012
A response study and the effects of different parameters (pH, temperature and enzyme dose) on kinetics of isolated soy protein hydrolysis by a trypsin-like endopeptidase (TL1) were conducted.
Zebin Wang, Jason Lombardi, Jessica Shaffer, Ted Wong   +3 more
doaj   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Enzyme kinetics for a two-step enzymic reaction with comparable initial enzyme-substrate ratios

, 1988
We extend the validity of the quasi-steady state assumption for a model double intermediate enzyme-substrate reaction to include the case where the ratio of initial enzyme to substrate concentration is not necessarily small.
P. K. Maini, Maini, P. K., Frenzen, C. L., Frenzen, CL, Maini, Philip, Maini, PK, C. L. Frenzen   +6 more
core   +1 more source

PENENTUAN KINETIKA ENZIM POLIGALAKTURONASE (PG) ENDOGENOUS DARI PULP BIJI KAKAO

Jurnal Biologi Udayana, 2014
This research was conducted to determine the enzyme kinetics (Km and Vmax) of endogenous polygalacturonase (PGs) isolated from cocoa pulp. This research was carried out by assaying PGs activities in various concentration of citrus pectin subtrates which ...
G.P. GANDA PUTRA
doaj  

Dynamic behavior of enzyme kinetics cooperative chemical reactions

AIP Advances
This article uses computational mathematics to investigate the dynamics of cooperative occurrences in chemical reactions inside living organisms. We study the dynamics of complex systems using mathematical models based on ordinary differential equations,
Akhtar Jan, Rehan Ali Shah, Hijaz Ahmad, Hazrat Bilal, Bandar Almohsen   +4 more
doaj   +1 more source