Results 71 to 80 of about 308,992 (299)
Numerical Solution for Fuzzy Enzyme Kinetic Equations by the Runge–Kutta Method
Mathematical and Computational Applications, 2018 One of the most important biochemical reactions is catalyzed by enzymes. A numerical method to solve nonlinear equations of enzyme kinetics, known as the Michaelis and Menten equations, together with fuzzy initial values is introduced.
Yousef Barazandeh, Bahman Ghazanfari
doaj +1 more source
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
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EI of the Phosphotransferase System of Escherichia coli: Mathematical Modeling Approach to Analysis of Its Kinetic Properties [PDF]
, 2011 The mathematical model of the operation of the first enzyme of theEscherichia coliphosphotransferase system, EI, is proposed. Parameters of the kinetic model describing the operation of EI under different conditions are identified on the basis of a large
T. A. Karelina +7 more
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On Over-Parameterisation and Parameter Estimation of Enzyme Kinetics
AppliedChemThe estimation of kinetic parameters based on experiments is an important element in understanding the reaction mechanisms of enzymes and their intrinsic properties.
Thomas Waluga, Paras Nagshi
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FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Estimating the turnover number in enzyme kinetic reactions using transient and stationary state data
Brazilian Journal of Pharmaceutical Sciences, 2009 Substrate and product concentration data obtained by simulating enzyme-substrate reaction rate equations were used to test two proposed kinetic rate constant estimation techniques in this study.
Sibel Uludag-Demirer +2 more
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FEBS Open Bio, EarlyView.This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake +4 more
wiley +1 more source
Tests of predictions made by the Equilibrium Model for the effect of temperature on enzyme activity
, 2008 The Classical Model describing the effects of temperature on enzyme activity consists of two processes: the catalytic reaction defined by ΔG cat and irreversible inactivation defined by ΔG inact, this model however, does not account for the observed ...
Oudshoorn, Matthew Leslie
core
, 2022 S.627-632 : Abb.,Lit.In order to establish the theoretically ideal conditions necessary to minimize enzyme requirement for a given process, a computer simulation of the ideal stirred tank reactor (CSTR), the ideal plug flow reactor (PFR) and the ideal ...
Chmiel, H., Kulbe, K.-D., Howaldt, M.
core
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source