Results 51 to 60 of about 308,992 (299)

Deconjugation Kinetics of Glucuronidated Phase II Flavonoid Metabolites by B-glucuronidase from Neutrophils [PDF]

, 2010
Flavonoids are inactivated by phase II metabolism and occur in the body as glucuronides. Mammalian ß-glucuronidase released from neutrophils at inflammatory sites may be able to deconjugate and thus activate flavonoid glucuronides.
Arts, I.C.W., Pieter C. Dagnelie, Needs, P.W., Paul A. Kroon, Jaap Keijer, Paul W. Needs, Keijer, J., Kroon, P.A., Roger Bartholomé, Hollman, P.C.H., Dirk Roos, Ilja C.W. Arts, Haenen, G., Peter C.H. Hollman, Dagnelie, P.C., Guido Haenen, Roos, D., Bartholomé, R., Bast, A., Aalt Bast   +19 more
core   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

Applying Thermodynamics, Enzyme Kinetics, and pH in Medical Biochemistry - A Team-Based Learning Exercise

MedEdPORTAL, 2010
This is a team-based learning (TBL) exercise used to review concepts and apply knowledge in thermodynamics, enzyme kinetics, and pH in a medical biochemistry course. It can be presented to classes on a course server or as a PowerPoint presentation.
Edward McKee, Selma Omer
doaj   +1 more source

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Electron transfer between complexes III and IV in S. cerevisiae mitochondrial membranes

FEBS Letters, EarlyView.
Mitochondrial oxidative phosphorylation in S. cerevisiae mitoplasts is limited by complex IV catalytic capacity, rather than two‐dimensional cytochrome c diffusion. At physiological cytochrome c : supercomplex ratios at salinity equivalent to that of 20 mm monovalent salt, activity is maximized, indicating that this low ionic strength accurately mimics
Ana Paula Lobez, Mikael Oliveberg, Peter Brzezinski   +2 more
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

FEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace, Alyssa Borges, Charles Arnaud, Tove Karlsson, Nina K. Bröker, Stefanie Barbirz, Marta Bally   +6 more
wiley   +1 more source

Enzyme dynamics and activity: timescale dependence of dynamical transitions in glutamate dehydrogenase solution

, 1999
We have examined the temperature dependence of motions in a cryosolution of the enzyme glutamate dehydrogenase (GDH) and compared these with activity.
Ferrand M, Daniel RM, Daniel, Roy, Thomas, Ferrand, Michel, Finney J L, Daniel, Roy,, Finney, John, Dunn R, Reat, Valerie, Reat V, Smith, Jeremy C., Smith, Jeremy, Finney, John L., Dunn, Rachel, Dunn, Rachel V., Smith JC, Daniel, Roy M.   +16 more
core   +1 more source

Kinetic modelling of the role of the aldehyde dehydrogenase enzyme and the breast cancer resistance protein in drug resistance and transport [PDF]

, 2011
A compartmental model for the in vitro uptake kinetics of the anti-cancer agent topotecan (TPT) has been extended from a previously published model. The extended model describes the drug activity and delivery of the pharmacologically active form to the ...
N.D. Evans, Evans, Neil D., M.J. Chappell, Errington, Rachel J., R.J. Errington, Smith, Paul J., Smith, Paul James, Atari, M. I., Chappell, M. J., P.J. Smith, Chappell, M. J. (Michael J.), M.I. Atari, Errington, Rachel Jane, Evans, N. D.   +13 more
core   +1 more source

Kinetics of Enzyme Biodegradation of New Synthesized Copolymers [PDF]

Bioautomation, 2005
Block copolymers of the poly-(hexanlactam)-co-block-poly-(?-valerolactone) from ABA-type were synthesized via anionic polymerization of hexanlactam (HL) with the sodium salt of hexanlactam (Na-HL) as an initiator and polymeric activator (PAC).
Rosa Mateva, Natalia Toncheva, Lubov Yotova   +2 more
doaj  

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

FEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source