Results 41 to 50 of about 1,681,787 (293)
FEBS Letters, EarlyView.We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace +6 more
wiley +1 more source
Biosensors, 2023 Absorbance spectroscopy finds many biomedical and physical applications ranging from studying the atomic and molecular details of the analyte to determination of unknown biological species and their concentration or activity in the samples.
Antony Jesuraj, Umer Hassan
doaj +1 more source
FEBS Letters, EarlyView.Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani +3 more
wiley +1 more source
Calcium and chloride function in oxygen evolution by photosystem II through bisubstrate enzyme kinetics and EPR spectroscopy of fluoride inhibition [PDF]
, 2013 Photosystem II (PSII) is the light-harvesting water oxidase of thylakoid membranes. The catalytic center where oxygen forms in PSII is located at the manganese cluster, Mn4O5Ca, which is highly conserved throughout nature as seen in cyanobacteria and in ...
NC DOCKS at The University of North Carolina at Greensboro +1 more
core
Enzyme dynamics and activity: timescale dependence of dynamical transitions in glutamate dehydrogenase solution [PDF]
, 1999 We have examined the temperature dependence of motions in a cryosolution of the enzyme glutamate dehydrogenase (GDH) and compared these with activity.
Ferrand M +22 more
core +1 more source
mAbs, 2020 The extracellular ATP/adenosine axis in the tumor microenvironment (TME) has emerged as an important immune-regulatory pathway. Nucleoside triphosphate diphosphohydrolase-1 (NTPDase1), otherwise known as CD39, is highly expressed in the TME, both on ...
Bradley N. Spatola +11 more
doaj +1 more source
Emerging experimental and computational methods for studying redox‐regulated structural transitions
FEBS Letters, EarlyView.Redox reactions can reshape proteins and alter how they behave in cells, with important consequences for health and disease. This review explores emerging experimental and computational approaches for discovering these redox‐sensitive protein switches, revealing their structural effects, and predicting their behavior, opening new opportunities to ...
Tasneem Rass +2 more
wiley +1 more source
DENATURATION OF NATIVE AND DEGLYCOSYLATED α-GALACTOSIDASES FROM Penicillium canescens BY GUANIDINE HYDROCHLORIDE [PDF]
Biotechnologia Acta, 2015 The aim of this work was the study of native and galactosidases from Penicillium canescens under denaturing conditions caused by guanidine hydrochloride.
Borzova N. V.
doaj +1 more source
Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding
FEBS Letters, EarlyView.In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança +2 more
wiley +1 more source
Enzyme activity and flexibility at very low hydration [PDF]
, 2005 Recent measurements have demonstrated enzyme activity at hydrations as low as 3%. This raises the question of whether hydration-induced enzyme flexibility is important for activity. Here, to address this, picosecond dynamic neutron scattering experiments
Kurkal, V. +9 more
core +1 more source