Results 21 to 30 of about 5,877,636 (396)

Tuning Immobilized Commercial Lipase Preparations Features by Simple Treatment with Metallic Phosphate Salts

Molecules, 2022
Four commercial immobilized lipases biocatalysts have been submitted to modifications with different metal (zinc, cobalt or copper) phosphates to check the effects of this modification on enzyme features. The lipase preparations were Lipozyme®TL (TLL-IM)
José R. Guimarães, Diego Carballares, Paulo W. Tardioli, Javier Rocha-Martin, Roberto Fernandez-Lafuente   +4 more
doaj   +1 more source

Data-driven enzyme immobilisation: a case study using DNA to immobilise galactose oxidase

Engineering Biology, 2020
Biocatalysis has the potential to enable green chemistry. New methods of enzyme immobilisation will be required to improve enzyme stability, product purification, and compatibility of different enzymes in the same reaction conditions.
Wolfgang Ott, Alessandro Ceccarelli, Jack Manning, Nicholas J. Turner, Robert Oppenheimer   +4 more
doaj   +1 more source

Enhanced Stability of Long-Living Immobilized Recombinant β-d-N-Acetyl-Hexosaminidase A on Polylactic Acid (PLA) Films for Potential Biomedical Applications

Journal of Functional Biomaterials, 2021
β-d-N-acetyl-hexosaminidase (Hex, EC 3.2.1.52) is an acid hydrolase that catalyzes the cleavage of the β-1,4 bond in N-acetyl-d-galactosamine (Gal-NAc) and N-acetyl-d-glucosamine (Glc-NAc) from the non-reducing end of oligosaccharides and glycoconjugates.
Eleonora Calzoni, Alessio Cesaretti, Nicolò Montegiove, Alessandro Di Michele, Carla Emiliani   +4 more
doaj   +1 more source

Cloning and expression of Staphylococcus simulans lysostaphin enzyme gene in Bacillus subtilis WB600

AIMS Microbiology, 2021
Lysostaphin is a glycylglycine endopeptidase, secreted by Staphylococcus simulans, capable of specifically hydrolyzing pentaglycine crosslinks present in the peptidoglycan of the Staphylococcus aureus cell wall. In this paper, we describe the cloning and
Babak Elyasi Far, Mehran Ragheb, Reza Rahbar , Ladan Mafakher , Neda Yousefi Nojookambari, Spyridon Achinas, Sajjad Yazdansetad   +6 more
doaj   +1 more source

Characterization of highly stable extracellular lipase from the extremely halophilic archaeon Halolamina sp.

Bioscience Journal, 2022
Enzymes of the archaea living in extreme environments are resistant to the challenging conditions. Lipase is among the important enzymes used in the industry and agriculture.
Sezer Okay, Şevki Adem, Aslıhan Kurt-Kizildoğan   +2 more
doaj   +1 more source

Freeze-Dried Tuna Pepsin Powder Stabilized by Some Cryoprotectants: In Vitro Simulated Gastric Digestion toward Different Proteins and Its Storage Stability

Foods, 2022
The impact of maltodextrin (10%) in combination with trehalose or glycerol at different levels (2.5% and 5%) and their mixture on the stability of freeze-dried pepsin from skipjack tuna stomach was studied.
Umesh Patil, Mehdi Nikoo, Bin Zhang, Soottawat Benjakul   +3 more
doaj   +1 more source

Assaying activity and assessing thermostability of hyperthermophilic enzymes [PDF]

, 2001
There is now a wide variety of intra- and extra-cellular enzymes available from organisms growing above 75°C, and having sufficient stability to allow assay well above this temperature.
Akanuma, Beynon, Daniel, Daniel, Daniel, Daniel, Danson, Dixon, Fourage, Gerike, Giver, Hensel, Hensel, Hudson, John, Laidler, More, Ramirez, Ratcliffe, Stevens, Tetas, Thomas, Walsh   +22 more
core   +2 more sources

Improvement in the Thermostability of a Recombinant β-Glucosidase Immobilized in Zeolite under Different Conditions

Molecules, 2022
β-Glucosidase is part of the cellulases and is responsible for degrading cellobiose into glucose, a compound that can be used to produce biofuels. However, the use of the free enzyme makes the process more expensive.
Luis Gerardo Ramírez-Ramírez, David Enrique Zazueta-Álvarez, Héctor Alonso Fileto-Pérez, Damián Reyes-Jáquez, Cynthia Manuela Núñez-Núñez, Juan de Dios Galindo-De la Rosa, Javier López-Miranda, Perla Guadalupe Vázquez-Ortega   +7 more
doaj   +1 more source

A new intrinsic thermal parameter for enzymes reveals true temperature optima [PDF]

, 2004
Two established thermal properties of enzymes are the Arrhenius activation energy and thermal stability. Arising from anomalies found in the variation of enzyme activity with temperature, a comparison has been made of experimental data for the activity ...
Daniel, Roy M., Danson, Michael J., Eisenthal, Robert, Peterson, Michelle E., Spence, Alastair   +4 more
core   +2 more sources

The Synthesis of (Magnetic) Crosslinked Enzyme Aggregates With Laccase, Cellulase, β-Galactosidase and Transglutaminase

Frontiers in Bioengineering and Biotechnology, 2022
Immobilized enzymes have important aspects due to the fact that they possess higher stability, have the possibility to be easily removed from the reaction mixture, and are much easier to use when compared to free enzymes.
Gordana Hojnik Podrepšek, Željko Knez, Željko Knez, Maja Leitgeb, Maja Leitgeb   +4 more
doaj   +1 more source