Results 21 to 30 of about 978,452 (311)

Enantiomeric Tartaric Acid Production Using cis-Epoxysuccinate Hydrolase: History and Perspectives

open access: yesMolecules, 2019
Tartaric acid is an important chiral chemical building block with broad industrial and scientific applications. The enantioselective synthesis of l(+)- and d(−)-tartaric acids has been successfully achieved using bacteria presenting cis ...
Jinsong Xuan, Yingang Feng
doaj   +1 more source

Activity-Stability Relationships in Extremophilic Enzymes [PDF]

open access: yesJournal of Biological Chemistry, 2003
Psychrophilic, mesophilic, and thermophilic alpha-amylases have been studied as regards their conformational stability, heat inactivation, irreversible unfolding, activation parameters of the reaction, properties of the enzyme in complex with a transition state analog, and structural permeability.
D'Amico, Salvino   +3 more
openaire   +2 more sources

Enhanced oral absorption of insulin: hydrophobic ion pairing and a self-microemulsifying drug delivery system using a D-optimal mixture design

open access: yesDrug Delivery, 2022
The lipophilicity of a peptide drug can be considerably increased by hydrophobic ion pairing with amphiphilic counterions for successful incorporation into lipid-based formulations.
Yoon Tae Goo   +8 more
doaj   +1 more source

Consensus mutagenesis reveals that non-helical regions influence thermal stability of horseradish peroxidase [PDF]

open access: yes, 2008
The enzyme horseradish peroxidase has many uses in biotechnology but a stabilized derivative would have even wider applicability. To enhance thermal stability, we applied consensus mutagenesis (used successfully with other proteins) to recombinant ...
Amin   +56 more
core   +1 more source

The development of human sera tests for HDL-bound serum PON1 and its lipolactonase activitys⃞

open access: yesJournal of Lipid Research, 2007
Serum paraoxonase (PON1) is a lipolactonase that associates with HDL-apolipoprotein A-I (HDL-apoA-I) and thereby plays a role in the prevention of atherosclerosis. Current sera tests make use of promiscuous substrates and provide no indications regarding
Leonid Gaidukov, Dan S. Tawfik
doaj   +1 more source

Astroenzymology – the environmental limits of enzyme activity [PDF]

open access: yes, 2003
Using organisms from extreme terrestrial environments as models for extraterrestrial life may lead us to underestimate the range of environments that life may inhabit.
Daniel, Roy M.
core   +2 more sources

Production of extracellular protease by a Brazilian strain of Beauveria bassiana reactivated on coffee berry borer, Hypothenemus hampei

open access: yesBrazilian Archives of Biology and Technology, 2007
Studies were carried out on extracellular protease production by Beauveria bassiana CG432 in liquid medium containing glucose and yeast extract. B. Bassiana presented active growth after lag period of 24 h., produced 80% of the total of the extracellular
Eliana Tiemi Ito   +4 more
doaj   +1 more source

Investigating on the Stability of Peroxidase Extracted from Soybean (Glycine max var. Williams) and Effects of Na+ and K+ Ions on its Activity [PDF]

open access: yesJournal of Cell and Molecular Research, 2015
In the present study, some techniques were used for Soybean peroxidase (SBP) purification including: ammonium sulfate fractionation, DEAE Sephadex anion exchange chromatography and Concanavalin A Sepharose 4B affinity chromatography.
Leila Parsiavash   +2 more
doaj   +1 more source

Optimization of extraction of novel pectinase enzyme discovered in red pitaya (Hylocereus polyrhizus) peel [PDF]

open access: yes, 2013
Plant peels could be a potential source of novel pectinases for use in various industrial applications due to their broad substrate specificity with high stability under extreme conditions. Therefore, the extraction conditions of a novel pectinase enzyme
Ahmad Zohdi, Nor Khanani   +1 more
core   +2 more sources

Escherichia coli Fails to Efficiently Maintain the Activity of an Important Flavin Monooxygenase in Recombinant Overexpression

open access: yesFrontiers in Microbiology, 2017
This paper describes the measurement and analysis of in vivo activity and stability of cyclohexanone monooxygenase from Acinetobacter sp. NCIMB 9871 (CHMO), a model Baeyer–Villiger monooxygenase, in the recombinant host Escherichia coli.
Sofia Milker   +4 more
doaj   +1 more source

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