Results 41 to 50 of about 2,250,202 (262)

Engineering a CRISPR-associated IscB system for developing miniature genome-editing tools in human cells and mouse embryos

Nature Communications
IscB, as the putative ancestor of Cas9, possesses a compact size, making it suitable for in vivo delivery. OgeuIscB is the first IscB protein known to function in eukaryotic cells but requires a complex TAM (NWRRNA).
Fei Zhang, Yuxin Peng, Dongdong Fan, Guoxu Song, Xing Gao, Yong Tian   +5 more
doaj   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

scIVNL-seq resolves in vivo single-cell RNA dynamics of immune cells during Salmonella infection

Nature Communications
The immune response against pathogens involves multiple cell state transitions and complex gene expression changes. Here, we establish a single-cell in vivo new RNA labeling sequencing method (scIVNL-seq) and apply it to survey time-resolved RNA dynamics
Zhen Xiong, Runyuan Wu, Yuanxin Wang, Yuwei Xu, Cunzhen Li, Deyuan Kong, Ziqi Xiao, Peikang Zhang, Zhonglong Wang, Peng Zhang, Ying Du, Hui Guo, Pingping Zhu, Shunmin He, Zusen Fan   +14 more
doaj   +1 more source

Epigenetic regulation in lung cancer

MedComm, 2023
AbstractLung cancer is indeed a major cause of cancer‐related deaths worldwide. The development of tumors involves a complex interplay of genetic, epigenetic, and environmental factors. Epigenetic mechanisms, including DNA methylation (DNAm), histone modifications, and microRNA expression, play a crucial role in this process.
Shahin Ramazi, Maedeh Dadzadi, Zahra Sahafnejad, Abdollah Allahverdi   +3 more
openaire   +3 more sources

Microbiome−host proteostasis crosstalk—An emerging perspective on mechanisms and interventions toward healthy longevity

FEBS Letters, EarlyView.
Proteostasis and the gut microbiota play a key role in shaping host physiology. Microbiota‐derived metabolites, vitamins, and RNA modulate host proteostasis. Findings from model systems, including C. elegans, indicate microbes can either stabilize or disrupt host proteostasis.
Abhishek Anil Dubey, Maria Ermolaeva
wiley   +1 more source

Cryo-ET comparison of the hierarchical ultrastructure of silkworm, spider, and artificial silk fibers

Nature Communications
Spider and silkworm silks are renowned for their exceptional mechanical properties, which arises from their ultrastructural organization. However, this architecture remains incompletely understood.
Kai Song, Haonan Zhang, Xueli Zhang, Yan Li, Ping Zhu   +4 more
doaj   +1 more source

pHYPER, a shRNA vector for high-efficiency RNA interference in embryonic stem cells

BioTechniques, 2007
RNA interference (RNAi) is a powerful method to generate loss-of-function phenotypes. Plasmid vectors with RNA polymerase III promoters have been developed to express short hairpin RNAs (shRNAs) in mammalian cells.
Soizik Berlivet, Virginie Guiraud, Martin Houlard, Matthieu Gérard   +3 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Genetic, environmental and stochastic factors in monozygotic twin discordance with a focus on epigenetic differences [PDF]

, 2012
Genetic-epidemiological studies on monozygotic (MZ) twins have been used for decades to tease out the relative contributions of genes and the environment to a trait.
Morahan, JM, Ramagopalan, SV, Ebers George C, Czyz Witold, Ramagopalan, Sreeram, George C Ebers, Sreeram V Ramagopalan, Ebers, GC, Ramagopalan Sreeram V, Witold Czyz, Julia M Morahan, Morahan Julia M, Ebers, George, Czyz, W   +13 more
core   +2 more sources

Dynamics and epigenetic control of PcG proteins during skeletal muscle cell differentiation [PDF]

, 2010
During skeletal muscle differentiation, signal dependent switches in cell differentiation programs require global rearrangements in repression and activation of lineage specific genes, hence the importance of unravelling epigenetic mechanisms that ...
Prezioso, Carolina
core   +1 more source