Results 121 to 130 of about 505,722 (385)

Molecular characterization and prediction of B-cell epitopes for the development of SARS-CoV-2 vaccine through bioinformatics approach [PDF]

Journal of Pharmacy & Pharmacognosy Research, 2022
Context: The SARS-CoV-2 virus is the cause of the COVID-19 pandemic, which is a severe public health crisis worldwide.Aims: To analyze the SARS-CoV-2 isolates of Surabaya and predict ORF1ab polyprotein epitopes through the bioinformatics approach for ...
Aamir Shehzad, Martia Rani Tacharina, Suryo Kuncorojakti, Hafiz Ishfaq Ahmad, Rofiqul A’la, Andi Yasmin Wijaya, Wiwiek Tyasningsih, Fedik Abdul Rantam   +7 more
doaj  

Epitope prediction improved by multitask support vector machines

, 2007
Motivation: In silico methods for the prediction of antigenic peptides binding to MHC class I molecules play an increasingly important role in the identification of T-cell epitopes. Statistical and machine learning methods, in particular, are widely used
Jacob, Laurent, Vert, Jean-Philippe
core   +1 more source

Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis [PDF]

, 2009
Antibodies to citrulline-modifi ed proteins have a high diagnostic value in rheumatoid arthritis (RA). However, their biological role in disease development is still unclear.
Aho, Andersson, Auger, Baeten, Bella, Bettina Sehnert, Brunsberg, Burkhardt, Burkhardt, Bäcklund, Caspi, Chang, Chapuy-Regaud, Collaborative Computational Project, Number 4, DeLano, Dyson, Emsley, Emsley, Emsley, Estelle Bajtner, Foulquier, Girbal-Neuhauser, Giudicelli, Grab, Guy Serre, Harald Burkhardt, Hill, Hill, Hoffmann, Hüseyin Uysal, Kabsch, Kanazawa, Kidd, Krissinel, Kuhn, Kutty S. Nandakumar, Lefranc, Marjolein M.G.M. Thunnissen, Masson-Bessière, Masson-Bessière, Michaëlsson, Molberg, Murshudov, Nair, Nandakumar, Rantapää-Dahlqvist, Read, Reparon-Schuijt, Rikard Holmdahl, Robert Bockermann, Rosloniec, Schellekens, Schellekens, Schulte, Schwab, Stanfield, Stetefeld, Tarcsa, Uysal, van der Helm-van Mil, Vander Cruyssen, von Delwig, Vossenaar, Vossenaar, Vossenaar, Vossenaar, Winn, Yousfi Monod, Zong, Åke Engström   +69 more
core   +2 more sources

Dissecting Antibodies with Regards to Linear and Conformational Epitopes

PLoS ONE, 2015
An important issue for the performance and specificity of an antibody is the nature of the binding to its protein target, including if the recognition involves linear or conformational epitopes.
Björn Forsström, Barbara Bisławska Axnäs, Johan Rockberg, H. Danielsson, A. Bohlin, M. Uhlén   +5 more
semanticscholar   +1 more source

Modifications in FLAP's second cytosolic loop influence 5‐LOX interaction, inhibitor binding, and leukotriene formation

FEBS Letters, EarlyView.
The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp, Katharina Rataj, Stefanie König, Marcia E. Newcomer, Oliver Werz, Ulrike Garscha   +5 more
wiley   +1 more source

Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid.

Proceedings of the National Academy of Sciences of the United States of America, 1984
A procedure is described for rapid concurrent synthesis on solid supports of hundreds of peptides, of sufficient purity to react in an enzyme-linked immunosorbent assay.
H. Geysen, Rob H. MELOENt, Simon J. BARTELINGt   +2 more
semanticscholar   +1 more source

The carboxylate “gripper” of the substrate is critical for C‐4 stereo‐inversion by UDP‐glucuronic acid 4‐epimerase

FEBS Letters, EarlyView.
UDP‐glucuronic acid 4‐epimerase (UGAepi) catalyzes NAD+‐dependent interconversion of UDP‐glucuronic acid (UDP‐GlcA) and UDP‐galacturonic acid (UDP‐GalA) via C4‐oxidation, 4‐keto‐intermediate rotation, and C4‐reduction. Here, Borg et al. examined the role of the substrate's carboxylate group in the enzymic mechanism by analyzing NADH‐dependent reduction
Annika J. E. Borg, Laura De Cnop, Bernd Nidetzky   +2 more
wiley   +1 more source

Western and dot immunoblotting analysis of viral antigens and antibodies: application to murine hepatitis virus. [PDF]

, 1984
Viral proteins were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred quantitatively to nitrocellulose by electroblotting in SDS-containing buffer.
Buchmeier, MJ, Knobler, RL, Talbot, PJ
core  

Development and validation of a broad scheme for prediction of HLA class II restricted T cell epitopes

ACM International Conference on Bioinformatics, Computational Biology and Biomedicine, 2014
Computational prediction of HLA class II restricted T cell epitopes has great significance in many immunological studies including vaccine discovery. With the development of novel bioinformatics approaches, prediction of HLA class II binding has improved
S. Paul, J. Sidney, Bjoern Peters, A. Sette   +3 more
semanticscholar   +1 more source

B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses

FEBS Letters, EarlyView.
B cells sense external mechanical forces and convert them into biochemical signals through mechanotransduction. Understanding how malignant B cells respond to physical stimuli represents a groundbreaking area of research. This review examines the key mechano‐related molecules and pathways in B lymphocytes, highlights the most relevant techniques to ...
Marta Sampietro, Marco Cellani, Cristina Scielzo   +2 more
wiley   +1 more source