Results 191 to 200 of about 5,392 (239)

Epitranscriptomic technologies and analyses

Science China Life Sciences, 2020
RNA can interact with RNA-binding proteins (RBPs), mRNA, or other non-coding RNAs (ncRNAs) to form complex regulatory networks. High-throughput CLIP-seq, degradome-seq, and RNA-RNA interactome sequencing methods represent powerful approaches to identify biologically relevant ncRNA-target and protein-ncRNA interactions.
Xiaoyu, Li, Qiao-Xia, Liang, Jin-Ran, Lin, Jinying, Peng, Jian-Hua, Yang, Chengqi, Yi, Yang, Yu, Qiangfeng Cliff, Zhang, Ke-Ren, Zhou   +8 more
openaire   +2 more sources

Single-molecule direct RNA sequencing reveals the shaping of epitranscriptome across multiple species

Nature Communications
N6-methyladenosine (m6A) is an essential RNA modification that regulates gene expression and influences diverse cellular processes. Yet, fully characterizing its transcriptome-wide landscape and biogenesis mechanisms remains challenging. Traditional next-
Yingyuan Xie, Zhen-Dong Zhong, Hong-Xuan Chen, Zengjun Ren, Yuan-Tao Qiu, Ye-Lin Lan, Fu Wu, Jin-Wen Kong, Ru-Jia Luo, Delong Zhang, Biao-Di Liu, Yang Shu, Feng Yin, Jian Wu, Zigang Li, Zhang Zhang, Guan-Zheng Luo   +16 more
semanticscholar   +1 more source

Epitranscriptome-epigenome interactions in development and disease mechanisms.

Trends in Genetics
Crosstalk between epitranscriptomic modifications to RNA and epigenomic modifications to DNA and histones plays fundamental roles in development and disease.
Chengyu Li, Kexuan Chen, Xiaoyu Li, Xushen Xiong   +3 more
semanticscholar   +1 more source

N4-acetylcytidine and other RNA modifications in epitranscriptome: insight into DNA repair and cancer development

Epigenomics
N4-acetylcytidine (ac4C) is a post-transcriptional RNA modification that plays a crucial role in the epitranscriptome, influencing gene expression and cellular function. This modification occurs at the cytosine base, where an acetyl group is installed to
E. Bártová, L. Stixová, Alena Svobodová Kovaříková   +2 more
semanticscholar   +1 more source

Epitranscriptomics in fibroblasts and fibrosis

American Journal of Physiology-Cell Physiology, 2022
Fibroblasts play an important role in the pathogenic mechanisms of several socially significant diseases, including pulmonary and cardiovascular fibrosis, liver cirrhosis, systemic sclerosis, progressive kidney disease. The alterations of the epitranscriptome, including more than 170 distinct posttranscriptional RNA modifications or editing events ...
Mirolyuba Ilieva, Shizuka Uchida
openaire   +3 more sources

N6-methyladenosine–encoded epitranscriptomics

Nature Structural & Molecular Biology, 2016
N6-methyladenosine (m6A) is the most abundant internal modification in eukaryotic mRNA. Recent discoveries of the locations, functions and mechanisms of m6A have shed light on a new layer of gene regulation at the RNA level, giving rise to the field of m6A epitranscriptomics. In this Perspective, we provide an update on the various effects of mammalian
Nian, Liu, Tao, Pan
openaire   +2 more sources

Targeting YTHDF2 impacts the epitranscriptome and overcomes tumor therapy resistance.

Trends in Cell Biology
Dysregulation of RNA modifiers is common across cancer types and has essential roles in tumor therapy resistance. Chen and colleagues reported that YTHDF2 played dual roles in B cell malignancies by enhancing cell proliferation and promoting immune ...
Zhicong Zhao, Li Han, Qiwei Ge
semanticscholar   +1 more source

Probing enzyme-dependent pseudouridylation using direct RNA sequencing to assess epitranscriptome plasticity in a neuronal cell line.

Cell Systems
Chemical modifications in mRNAs, such as pseudouridine (psi), can control gene expression. Yet, we know little about how they are regulated, especially in neurons.
Oleksandra Fanari, Sepideh Tavakoli, Yuchen Qiu, Amr Makhamreh, Keqing Nian, Stuart Akeson, Michele Meseonznik, Caroline A. McCormick, Dylan Bloch, Howard Gamper, Miten Jain, Ya-Ming Hou, M. Wanunu, Sara H. Rouhanifard   +13 more
semanticscholar   +1 more source

Bioinformatic tools for epitranscriptomics

American Journal of Physiology-Cell Physiology, 2023
The epitranscriptome, defined as RNA modifications that do not involve alterations in the nucleotide sequence, is a popular topic in the genomic sciences. Because we need massive computational techniques to identify epitranscriptomes within individual transcripts, many tools have been developed to infer epitranscriptomic sites as well as to process ...
openaire   +2 more sources

Resolving m6A epitranscriptome with stoichiometry

Trends in Genetics, 2022
A recent study by Hu et al. describes N6-methyladenosine (m6A)-selective allyl chemical labeling and sequencing (m6A-SAC-seq), which allows for quantitative, stoichiometric, and positional analyses of m6A at single-nucleotide resolution across the whole transcriptome level.
Ki-Jun Yoon, Yoon Ki Kim
openaire   +2 more sources