Results 81 to 90 of about 9,966 (237)

Evidence for a Prepore Stage in the Action of Clostridium perfringens Epsilon Toxin

PLoS ONE, 2011
Clostridium perfringens epsilon toxin (ETX) rapidly kills MDCK II cells at 37°C, but not 4°C. The current study shows that, in MDCK II cells, ETX binds and forms an oligomeric complex equally well at 37°C and 4°C but only forms a pore at 37°C. However, the complex formed in MDCK cells treated with ETX at 4°C has the potential to form an active pore ...
Robertson, SL, Li, J, Uzal, FA, McClane, BA   +3 more
openaire   +6 more sources

RHINO: An Integrative Multi‐Omics Framework Linking Circadian Physiology to Precision Medicine

Advanced Science, EarlyView.
RHINO (RHythmic Interacting Network for multi‐Omics) is an integrative framework that maps circadian regulation across diverse genetic and disease contexts and prioritizes druggable circadian targets. Released as an AI‐powered interactive web portal, RHINO unifies genetic, regulatory, disease, and drug–target information, enabling context‐specific ...
Ying Chen, Chengxuan Chen, Dishu Zhou, Panpan Liu, Roberto E. López‐Valiente, Yuan Liu, Cam Mong La, Isabella Beraldo Xavier, Sean M. Hartig, Pradip Saha, Zheng Sun, Leng Han, Dongyin Guan   +12 more
wiley   +1 more source

Fusion of Clostridium perfringens type D and B epsilon and beta toxin genes and it’s cloning in E. coli

Archives of Razi Institute, 2011
Designing and producing a proper fusion construction is the most important problem of producing large quantities of a properly folded functional protein. This construction should have all necessary components of a real gene.
Goudarzi, H.,, Habibi, G.R.,, Jabbari, A.R., Shamsara, M., Aghaei Pour, K., Pilehchian Langroudi, R., Ghorashi, S.A.   +6 more
doaj  

Stretchable, Tough, and Luminescent Perovskite Molecular Ferroelectric Composite: Empowering Reliable Self‐Powered Motion Sensing in High Humidity and Subzero Temperatures

Advanced Science, EarlyView.
A flexible molecular ferroelectric composite is fabricated via in situ growth of luminescent DMAA‐MnCl3 ferroelectric crystals within a SEBS/SIS double‐polymer matrix. It delivers 7.54 MPa tensile strength, over 1200% tensile strain, durable piezoelectric motion sensing, and excellent harsh‐environment stability for all‐weather wearable biomechanical ...
Shuangqing Li, Zhe‐Kun Xu, Zheng Xing, Jin‐Wang Liu, Yong‐Quan Wu, Peng‐Fei Li, Zhong‐Xia Wang   +6 more
wiley   +1 more source

Produção e caracterização de anticorpos monoclonais contra toxina épsilon de Clostridium perfringens Tipo D Production and characterization of monoclonal antibodies against Clostridium perfringens Type D epsilon toxin

Ciência Rural, 2009
Clostridium perfringens tipo D é o agente etiológico da enterotoxemia em ruminantes, causada pela toxina épsilon e caracterizada por edema cardíaco, pulmonar, renal e cerebral. Anticorpos monoclonais contra toxina épsilon de C.
Theonys Diógenes Freitas, Luis Guilherme Dias Heneine, Felipe Masiero Salvarani, Rodrigo Otávio Silveira Silva, Catarina Guimarães Rocha Dourado Lima, Prhiscylla Sadanã Pires, Ronnie Antunes de Assis, Francisco Carlos Faria Lobato   +7 more
doaj  

Detection of Different Genotypes of Clostridium perfringens in Feces of Healthy Dairy Cattle from China using Real-Time Duplex PCR Assay [PDF]

Pakistan Veterinary Journal, 2011
Dual-labeled fluorescence hybridization probe-based multiplex quantitative real-time polymerase chain reaction (qPCR) assay was used for the detection of Clostridium perfringens toxin genes alpha (cpa), beta (cpb), iota (ia), epsilon (etx), beta2 (cpb2 ...
Guanghua Wang, Jizhang Zhou, Fuying Zheng, Guozhen Lin, Xiaoan Cao, Xiaowei Gong and Changqing Qiu*
doaj  

Engineering Synergistic Intra‐/Intermolecular Conformational Locking via Side‐Chain Branching: A β‐Sheet‐Inspired Strategy for Planar NIR‐II Phototheranostic Aggregates

Advanced Science, EarlyView.
A bio‐inspired side‐chain branching strategy is developed to orchestrate a dual noncovalent conformational locking (NoCL) network, enabling the construction of perfectly planar NIR‐II phototheranostic aggregates. Such structural precision maximizes light‐harvesting efficiency and optimizes exciton dynamics, ensuring synchronized enhancements in NIR‐II ...
Cheng Lu, Qian Jia, Bo Wu, Chao Li, Ryan T. K. Kwok, Tie‐Gen Chen, Jianwei Sun, Zheng Zhao, Teng‐Teng Chen, Zhongliang Wang, Jacky W. Y. Lam, Ben Zhong Tang   +11 more
wiley   +1 more source

Calcium enhances binding of Clostridium perfringens epsilon toxin to sulfatide

Biochimica et Biophysica Acta (BBA) - Biomembranes, 2019
Epsilon toxin (Etx) from Clostridium perfringens is synthesized as a very low-active prototoxin form (proEtx) that becomes active upon proteolytic activation and has the capacity to cross the blood-brain barrier (BBB), thereby producing severe neurological effects.
C, Gil, J, Dorca-Arévalo, J, Blasi
openaire   +2 more sources

Multifunctional Manganese Oxide Nanocomposite Hydrogel With Synergistic Reactive Oxygen Species‐Scavenging, Oxygen‐Generating, Immunomodulatory, and Photothermal Antimicrobial Activities for Enhanced Diabetic Wound Healing

Advanced Science, EarlyView.
CFP/PC@MnO2 hydrogel integrates ROS scavenging, oxygen delivery, oxidative stress reduction, immunomodulation, and photothermal antibacterial properties. The incorporation of PC@MnO2 NPs endows the hydrogel with multiple functional advantages tailored to the pathological microenvironment of diabetic wounds, including efficient ROS clearance, mitigation
Zhi Xu, Xiaodong Luo, Jingxian Wu, Chaoyang Huang, Zhuoying Yang, Yixiang HePeng, Bosong Zhou, Xiang Li, Ruiyuan Liu, Xu Wu   +9 more
wiley   +1 more source

Chromatin Packing Domain Engineering Through the Manipulation of Nuclear Cationic States

Advanced Science, EarlyView.
Manipulation of intranuclear divalent cation concentrations rapidly and reversibly remodels chromatin packing domains in living cells. Magnesium depletion disrupts domain compaction, heterochromatin organization, and transcriptional regulation, whereas magnesium loading promotes more compact and stable domains.
Cody L. Dunton, Carrillo Paola Gonzalez, Luay Almassalha, Wing Shun Li, Ruyi Gong, Nicolas Acosta, I Chae Ye, Jane Frederick, Christopher Hogg, Saira John, J. A. Gareth Williams, Rikkert J. Nap, Igal Szleifer, Vadim Backman   +13 more
wiley   +1 more source