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Production of recombinant proteins in Escherichia coli [PDF]
Genetics and Molecular Biology, 2004 Attempts to obtain a recombinant protein using prokaryotic expression systems can go from a rewarding and rather fast procedure to a frustrating time-consuming experience.
Wolfgang Schumann +1 more
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The Protein Interactome of Glycolysis in Escherichia coli [PDF]
Proteomes, 2021 Glycolysis is regulated by numerous mechanisms including allosteric regulation, post-translational modification or protein-protein interactions (PPI). While glycolytic enzymes have been found to interact with hundreds of proteins, the impact of only some of these PPIs on glycolysis is well understood. Here we investigate which of these interactions may
Shomeek Chowdhury +4 more
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Proteins induced by aerobiosis in Escherichia coli [PDF]
Journal of Bacteriology, 1983 The role of protein induction and repression in the adaptation of Escherichia coli to changes in the supply of oxygen and other electron acceptors is only poorly understood. We have studied the changes in cellular protein composition associated with this adaptation by measuring the levels of 170 individual polypeptides produced during aerobic or ...
F C Neidhardt, M W Smith
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Optimization of Col H Gene Encoding Clostridium histolyticum Collagenase to Express in Escherichia coli [PDF]
Journal of Health Sciences and Surveillance System, 2022 Background: A given amino acid sequence can be encoded by a huge number of different nucleic acid sequences. These sequences, however, have proved not to be equally useful.
Seyed Mohammad Amin Mahdian +3 more
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Enzymatic and Non-Enzymatic Response during Nitrosative Stress in Escherichia coli
Postępy Mikrobiologii, 2022 Nitrosative stress is an adverse physiological condition mediated by an excessive level of reactive nitrogen species (RNS). RNS react with the different macromolecules in vivo and result in the inactivation of these molecules.
Nath Rohan +2 more
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Chimeras of Escherichia coli and Mycobacterium tuberculosis single-stranded DNA binding proteins: characterization and function in Escherichia coli. [PDF]
PLoS ONE, 2011 Single stranded DNA binding proteins (SSBs) are vital for the survival of organisms. Studies on SSBs from the prototype, Escherichia coli (EcoSSB) and, an important human pathogen, Mycobacterium tuberculosis (MtuSSB) had shown that despite significant ...
Sanjay Kumar Bharti +4 more
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Engineering Escherichia coli for efficient assembly of heme proteins
Microbial Cell Factories, 2023 Background Heme proteins, such as hemoglobin, horseradish peroxidase and cytochrome P450 (CYP) enzyme, are highly versatile and have widespread applications in the fields of food, healthcare, medical and biological analysis.
Jianzhong Ge +11 more
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Producing GST-Cbx7 Fusion Proteins from Escherichia coli
Bio-Protocol, 2017 This protocol describes the production of GST-Cbx7 fusion proteins from E. coli, originally developed in the recent publication (Zhen et al., 2016). The pGEX-6P-1-GST plasmids encoding the Cbx7 variants were transformed into BL21 competent cells.
Thao Huynh, Xiaojun Ren
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Microorganisms, 2022 Colibacillosis caused by pathogenic Escherichia coli (E. coli) is one of the most serious infectious diseases, causing an extensive burden on animal husbandry and the human healthcare system. Vaccination is one of the ideal ways to prevent E.
Guihong Pen +5 more
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Site-specific Incorporation of Phosphoserine into Recombinant Proteins in Escherichia coli
Bio-Protocol, 2022 This protocol describes the recombinant expression of proteins in E. coli containing phosphoserine (pSer) installed at positions guided by TAG codons. The E.
Phillip Zhu, Ryan Mehl, Richard Cooley
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