Recombinant protein production data after expression in the bacterium Escherichia coli
Fusion proteins have become essential for the expression and purification of recombinant proteins in Escherichia coli. The metal-binding protein CusF has shown several features that make it an attractive fusion protein and affinity tag: ''Expression and ...
J. Enrique Cantu-Bustos +5 more
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Optimization of Col H Gene Encoding Clostridium histolyticum Collagenase to Express in Escherichia coli [PDF]
Background: A given amino acid sequence can be encoded by a huge number of different nucleic acid sequences. These sequences, however, have proved not to be equally useful.
Seyed Mohammad Amin Mahdian +3 more
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An antifungal protein from Escherichia coli [PDF]
A cytosolic protein was purified fromEscherichia coliBL21 that demonstrated potent antifungal activity against pathogenic strains ofAspergillus fumigatus,Aspergillus flavus,Aspergillus nigerandCandida albicans. The MIC of purified protein fromE. coliBL21 (PPEBL21) againstAspergillusspecies andC.
V, Yadav +8 more
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Enzymatic and Non-Enzymatic Response during Nitrosative Stress in Escherichia coli
Nitrosative stress is an adverse physiological condition mediated by an excessive level of reactive nitrogen species (RNS). RNS react with the different macromolecules in vivo and result in the inactivation of these molecules.
Nath Rohan +2 more
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Chimeras of Escherichia coli and Mycobacterium tuberculosis single-stranded DNA binding proteins: characterization and function in Escherichia coli. [PDF]
Single stranded DNA binding proteins (SSBs) are vital for the survival of organisms. Studies on SSBs from the prototype, Escherichia coli (EcoSSB) and, an important human pathogen, Mycobacterium tuberculosis (MtuSSB) had shown that despite significant ...
Sanjay Kumar Bharti +4 more
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EcoProDB: the Escherichia coli protein database [PDF]
AbstractSummary: EcoProDB is a web-based database for comparative proteomics of Escherichia coli. The database contains information on E. coli proteins identified on 2D gels along with other resources collected from various databases and published literature, with a special feature of showing the expression levels of E.
Yun, Hongseok +6 more
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Engineering Escherichia coli for efficient assembly of heme proteins
Background Heme proteins, such as hemoglobin, horseradish peroxidase and cytochrome P450 (CYP) enzyme, are highly versatile and have widespread applications in the fields of food, healthcare, medical and biological analysis.
Jianzhong Ge +11 more
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Transport proteins promoting Escherichia coli pathogenesis [PDF]
Escherichia coli is a genetically diverse species infecting hundreds of millions of people worldwide annually. We examined seven well-characterized E. coli pathogens causing urinary tract infections, gastroenteritis, pyelonephritis and haemorrhagic colitis.
Tang, Fengyi, Saier, Milton H
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Production of recombinant proteins in Escherichia coli
Attempts to obtain a recombinant protein using prokaryotic expression systems can go from a rewarding and rather fast procedure to a frustrating time-consuming experience.
Wolfgang Schumann +1 more
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Mutant MotB proteins in Escherichia coli [PDF]
The MotB protein of Escherichia coli is an essential component in each of eight torque generators in the flagellar rotary motor. Based on its membrane topology, it has been suggested that MotB might be a linker that fastens the torque-generating machinery to the cell wall.
D F, Blair, D Y, Kim, H C, Berg
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