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Escherichia coli-derived virus-like particles in vaccine development

npj Vaccines, 2017
Recombinant virus-like particle-based vaccines are composed of viral structural proteins and mimic authentic native viruses but are devoid of viral genetic materials. They are the active components in highly safe and effective vaccines for the prevention
Xiaofen Huang, Xin Wang, Jun Zhang, Ningshao Xia, Qinjian Zhao   +4 more
doaj   +2 more sources

Recombinant protein expression in Escherichia coli: advances and challenges

Frontiers in Microbiology, 2014
Escherichia coli is the organism of choice for the production of recombinant proteins. Its use as a cell factory is well-established and it has become the most popular expression platform.
Germán Leandro Rosano, Germán Leandro Rosano, Eduardo A. Ceccarelli, Eduardo A. Ceccarelli   +3 more
doaj   +2 more sources

Total synthesis of Escherichia coli with a recoded genome

Nature, 2019
Julius Fredens, Kaihang Wang, Daniel De La Torre   +2 more
exaly   +2 more sources

Optimization of Col H Gene Encoding Clostridium histolyticum Collagenase to Express in Escherichia coli [PDF]

Journal of Health Sciences and Surveillance System, 2022
Background: A given amino acid sequence can be encoded by a huge number of different nucleic acid sequences. These sequences, however, have proved not to be equally useful.
Seyed Mohammad Amin Mahdian, Abbasali Raz, Navid Dinparast Djadid, Hamzeh Alipour   +3 more
doaj   +1 more source

Enzymatic and Non-Enzymatic Response during Nitrosative Stress in Escherichia coli

Postępy Mikrobiologii, 2022
Nitrosative stress is an adverse physiological condition mediated by an excessive level of reactive nitrogen species (RNS). RNS react with the different macromolecules in vivo and result in the inactivation of these molecules.
Nath Rohan, Sengupta Swarnab, Bhattacharjee Arindam   +2 more
doaj   +1 more source

Chimeras of Escherichia coli and Mycobacterium tuberculosis single-stranded DNA binding proteins: characterization and function in Escherichia coli. [PDF]

PLoS ONE, 2011
Single stranded DNA binding proteins (SSBs) are vital for the survival of organisms. Studies on SSBs from the prototype, Escherichia coli (EcoSSB) and, an important human pathogen, Mycobacterium tuberculosis (MtuSSB) had shown that despite significant ...
Sanjay Kumar Bharti, Kervin Rex, Pujari Sreedhar, Neeraja Krishnan, Umesh Varshney   +4 more
doaj   +1 more source

Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications

Frontiers in Bioengineering and Biotechnology, 2021
Recombinant proteins are becoming increasingly important for industrial applications, where Escherichia coli is the most widely used bacterial host for their production. However, the formation of inclusion bodies is a frequently encountered challenge for
Arshpreet Bhatwa, Weijun Wang, Y. Hassan, Nadine Abraham, Xiu-zhen Li, Ting Zhou   +5 more
semanticscholar   +1 more source

Engineering Escherichia coli for efficient assembly of heme proteins

Microbial Cell Factories, 2023
Background Heme proteins, such as hemoglobin, horseradish peroxidase and cytochrome P450 (CYP) enzyme, are highly versatile and have widespread applications in the fields of food, healthcare, medical and biological analysis.
Jianzhong Ge, Xiaolu Wang, Yingguo Bai, Yaru Wang, Yuan Wang, Tao Tu, Xing Qin, Xiaoyun Su, Huiying Luo, Bin Yao, Huoqing Huang, Jie Zhang   +11 more
doaj   +1 more source

Production of recombinant proteins in Escherichia coli

Genetics and Molecular Biology, 2004
Attempts to obtain a recombinant protein using prokaryotic expression systems can go from a rewarding and rather fast procedure to a frustrating time-consuming experience.
Wolfgang Schumann, Luis Carlos S. Ferreira   +1 more
doaj   +1 more source

The Outer Membrane Proteins and Their Synergy Triggered the Protective Effects against Pathogenic Escherichia coli

Microorganisms, 2022
Colibacillosis caused by pathogenic Escherichia coli (E. coli) is one of the most serious infectious diseases, causing an extensive burden on animal husbandry and the human healthcare system. Vaccination is one of the ideal ways to prevent E.
Guihong Pen, Na Yang, Da Teng, Ya Hao, Ruoyu Mao, Jianhua Wang   +5 more
doaj   +1 more source