Adaptations of Escherichia coli strains to oxidative stress are reflected in properties of their structural proteomes. [PDF]
, 2020 BACKGROUND:The reconstruction of metabolic networks and the three-dimensional coverage of protein structures have reached the genome-scale in the widely studied Escherichia coli K-12 MG1655 strain.
Catoiu, Edward +6 more
core +2 more sources
Bacterial riboproteogenomics : the era of N-terminal proteoform existence revealed [PDF]
, 2020 With the rapid increase in the number of sequenced prokaryotic genomes, relying on automated gene annotation became a necessity. Multiple lines of evidence, however, suggest that current bacterial genome annotations may contain inconsistencies and are ...
Fijalkowska, Daria +3 more
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Structure of the PII signal transduction protein of Neisseria meningitidis at 1.85 Ȃ resolution [PDF]
, 2006 Copyright @ 2006 International Union of CrystallographyThe PII signal transduction proteins GlnB and GlnK are implicated in the regulation of nitrogen assimilation in Escherichia coli and other enteric bacteria.
Alderton, D +6 more
core +1 more source
Recombinant protein production data after expression in the bacterium Escherichia coli
Data in Brief, 2016 Fusion proteins have become essential for the expression and purification of recombinant proteins in Escherichia coli. The metal-binding protein CusF has shown several features that make it an attractive fusion protein and affinity tag: ''Expression and ...
J. Enrique Cantu-Bustos +5 more
doaj +1 more source
Microorganisms, 2022 Escherichia coli (E. coli) is one of the most common pathogenic bacteria worldwide. Avian pathogenic E. coli (APEC) causes severe systemic disease in poultry (Colibacillosis), and accordingly, has an extreme risk to the poultry industry and public health
Fatma Abdelrahman +6 more
doaj +1 more source
Quantifying the benefit of a proteome reserve in fluctuating environments. [PDF]
, 2017 The overexpression of proteins is a major burden for fast-growing bacteria. Paradoxically, recent characterization of the proteome of Escherichia coli found many proteins expressed in excess of what appears to be optimal for exponential growth.
Erickson, David W +4 more
core +3 more sources
Bioinformatic and Molecular Analysis of Inverse Autotransporters from
mSphere, 2019 Proteins secreted by the type V secretion system possess multiple functions, including the capacity to mediate adhesion, aggregation, and biolfilm formation.
Kelvin G. K. Goh +4 more
doaj +1 more source
Producing GST-Cbx7 Fusion Proteins from Escherichia coli
Bio-Protocol, 2017 This protocol describes the production of GST-Cbx7 fusion proteins from E. coli, originally developed in the recent publication (Zhen et al., 2016). The pGEX-6P-1-GST plasmids encoding the Cbx7 variants were transformed into BL21 competent cells.
Thao Huynh, Xiaojun Ren
doaj +1 more source
Transforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells. [PDF]
PLoS ONE, 2010 A previously engineered Methanocaldococcus jannaschii tRNA(CUA Tyr)-tyrosyl-tRNA synthetase pair orthogonal to Escherichia coli was modified to become orthogonal in mammalian cells.
Gabrielle Nina Thibodeaux +6 more
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Type III Secretion Effectors with Arginine N-Glycosyltransferase Activity [PDF]
, 2020 Type III secretion systems are used by many Gram-negative bacterial pathogens to inject proteins, known as effectors, into the cytosol of host cells. These virulence factors interfere with a diverse array of host signal transduction pathways and cellular
Araujo Garrido, Juan Luis +2 more
core +1 more source