Transforming a pair of orthogonal tRNA-aminoacyl-tRNA synthetase from Archaea to function in mammalian cells. [PDF]
PLoS ONE, 2010 A previously engineered Methanocaldococcus jannaschii tRNA(CUA Tyr)-tyrosyl-tRNA synthetase pair orthogonal to Escherichia coli was modified to become orthogonal in mammalian cells.
Gabrielle Nina Thibodeaux +6 more
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Proteins induced by aerobiosis in Escherichia coli [PDF]
Journal of Bacteriology, 1983 The role of protein induction and repression in the adaptation of Escherichia coli to changes in the supply of oxygen and other electron acceptors is only poorly understood. We have studied the changes in cellular protein composition associated with this adaptation by measuring the levels of 170 individual polypeptides produced during aerobic or ...
M W, Smith, F C, Neidhardt
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Engineered Recombinant Protein Production Systems Originated from Escherichia coli
Trends in Peptide and Protein Sciences, 2019 Selection of a suitable host strain is an important step in recombinant production of heterologous proteins. As the prokaryotic systems and especially Escherichia coli (E.
Javad Ranjbari
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Active Protein Aggregates Produced in Escherichia coli [PDF]
International Journal of Molecular Sciences, 2011 Since recombinant proteins are widely used in industry and in research, the need for their low-cost production is increasing. Escherichia coli is one of the best known and most often used host organisms for economical protein production. However, upon over-expression, protein aggregates called inclusion bodies (IBs) are often formed. Until recently IBs
Peternel, Špela, Komel, Radovan
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Comparative proteomics of uropathogenic Escherichia coli during growth in human urine identify UCA-like (UCL) fimbriae as an adherence factor involved in biofilm formation and binding to uroepithelial cells [PDF]
, 2015 Uropathogenic Escherichia coli (UPEC) are the primary cause of urinary tract infection (UTI) in humans. For the successful colonisation of the human urinary tract, UPEC employ a diverse collection of secreted or surface-exposed virulence factors ...
Allsopp, LP +5 more
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Isolate Specific Cold Response of Yersinia enterocolitica in Transcriptional, Proteomic, and Membrane Physiological Changes [PDF]
, 2020 Yersinia enterocolitica, a zoonotic foodborne pathogen, is able to withstand low temperatures. This psychrotrophic ability allows it to multiply in food stored in refrigerators. However, little is known about the Y. enterocolitica cold response.
Alter, Thomas +4 more
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Roles of transcriptional and translational control mechanisms in regulation of ribosomal protein synthesis in Escherichia coli [PDF]
, 2017 Bacterial ribosome biogenesis is tightly regulated to match nutritional conditions and to prevent formation of defective ribosomal particles. InEscherichia coli, most ribosomal protein (r-protein) synthesis is coordinated with rRNA synthesis by a ...
Burgos, Hector L +3 more
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Site-specific Incorporation of Phosphoserine into Recombinant Proteins in Escherichia coli
Bio-Protocol, 2022 This protocol describes the recombinant expression of proteins in E. coli containing phosphoserine (pSer) installed at positions guided by TAG codons. The E.
Phillip Zhu, Ryan Mehl, Richard Cooley
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Integrated dataset on acute phase protein response in chicken challenged with Escherichia coli lipopolysaccharide endotoxin [PDF]
, 2018 Data herein describe the quantitative changes in the plasma proteome in chickens challenged with lipopolysaccharide (LPS), a bacterial endotoxin known to stimulate the host innate immune system obtained by shotgun quantitative proteomic tandem mass tags ...
Bain, Maureen +7 more
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Producción de proteínas recombinantes de Plasmodium falciparum en Escherichia coli
Biomédica: revista del Instituto Nacional de Salud, 2016 Introducción. La producción de proteínas recombinantes es fundamental para el estudio funcional de las proteínas de Plasmodium falciparum. Sin embargo, las proteínas recombinantes de P.
Ángela Patricia Guerra +3 more
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