Engineered Recombinant Protein Production Systems Originated from Escherichia coli
Trends in Peptide and Protein Sciences, 2019 Selection of a suitable host strain is an important step in recombinant production of heterologous proteins. As the prokaryotic systems and especially Escherichia coli (E.
Javad Ranjbari
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Site-specific Incorporation of Phosphoserine into Recombinant Proteins in Escherichia coli
Bio-Protocol, 2022 This protocol describes the recombinant expression of proteins in E. coli containing phosphoserine (pSer) installed at positions guided by TAG codons. The E.
Phillip Zhu, Ryan Mehl, Richard Cooley
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NMR Studies of Escherichia Coli Acyl Carrier Protein: Dynamic and Structural Differences of the Apo- and Holo-forms [PDF]
, 2006 Two indicators of conformational variability of Escherichia coli acyl carrier protein (ACP) have been investigated, namely backbone dynamics and chemical shift variations of ACP.
Eletr, Ziad +2 more
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DNA Sampling: a method for probing protein binding at specific loci on bacterial chromosomes [PDF]
, 2009 We describe a protocol, DNA sampling, for the rapid isolation of specific segments of DNA, together with bound proteins, from Escherichia coli K-12. The DNA to be sampled is generated as a discrete fragment within cells by the yeast I-SceI meganuclease ...
Blattner +29 more
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Data in Brief, 2016 This article presents data that were obtained through measuring the impact of the Min oscillation on membrane proteins in Escherichia coli by quantitative protemoics analysis.
Suh-Yuen Liang +3 more
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SoluProt: prediction of soluble protein expression in Escherichia coli
Bioinform., 2020 Motivation Poor protein solubility hinders the production of many therapeutic and industrially useful proteins. Experimental efforts to increase solubility are plagued by low success rates and often reduce biological activity. Computational prediction of
J. Hon +6 more
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Microarray-based screening of differentially expressed genes of E. coli O157:H7 Sakai during preharvest survival on butterhead lettuce [PDF]
, 2016 Numerous outbreaks of Escherichia coli O157:H7 have been linked to the consumption of leafy vegetables. However, up to the present, little has been known about E.
Cottyn, Bart +6 more
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Production of a subunit vaccine candidate against porcine post-weaning diarrhea in high-biomass transplastomic tobacco [PDF]
, 2012 Post-weaning diarrhea (PWD) in piglets is a major problem in piggeries worldwide and results in severe economic losses. Infection with Enterotoxigenic Escherichia coli (ETEC) is the key culprit for the PWD disease.
Cox, Eric +5 more
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Nature Communications, 2016 Several proteins are expressed at too low abundance in the Escherichia coli (E. coli) proteome to be detected by standard methods. Here, the authors create a microfluidics-based platform enabling single-molecule counting of low-abundance proteins by ...
Burak Okumus +11 more
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Producción de proteínas recombinantes de Plasmodium falciparum en Escherichia coli
Biomédica: revista del Instituto Nacional de Salud, 2016 Introducción. La producción de proteínas recombinantes es fundamental para el estudio funcional de las proteínas de Plasmodium falciparum. Sin embargo, las proteínas recombinantes de P.
Ángela Patricia Guerra +3 more
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