Results 61 to 70 of about 19,636 (235)
Relaxation of Loaded ESCRT-III Spiral Springs Drives Membrane Deformation [PDF]
, 2015 International audienceESCRT-III is required for lipid membrane remodeling in many cellular processes, from abscission to viral budding and multi-vesicular body biogenesis. However, how ESCRT-III polymerization generates membrane curvature remains debated.
Redondo-Morata, Lorena +9 more
core +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
ESCRT-II functions by linking to ESCRT-I in human immunodeficiency virus-1 budding. [PDF]
, 2019 Human immunodeficiency virus (HIV) uses the ESCRT (endosomal sorting complexes required for transport) protein pathway to bud from infected cells. Despite the roles of ESCRT-I and -III in HIV budding being firmly established, participation of ESCRT-II in
Ip, Natasha C.Y. +4 more
core +2 more sources
Advanced Materials Interfaces, EarlyView.Dynamic mechanical loading of outer meniscus fibrochondrocytes increases extracellular vesicle (EV) yield and alters EV composition. When applied to progenitor cells, these mechanically primed EVs modulate the expression of matrix‐related genes.
Catherine Cheung +6 more
wiley +1 more source
ESCRT Complexes Assembled and GLUEd [PDF]
, 2006 The ESCRT-I, -II, and -III complexes act sequentially to sort monoubiquitinated transmembrane proteins into multivesicular bodies for targeted degradation in the lysosome.
David G. Lambright +3 more
core +1 more source
ESCRT-I fuels lysosomal degradation to restrict TFEB/TFE3 signaling via the Rag-mTORC1 pathway
Life Science Alliance, 2022 ESCRT-I deficiency impairs lysosome membrane turnover and induces homeostatic responses to lysosomal nutrient starvation including activation of MiT-TFE signaling caused by inhibition of the substrate-specific mTORC1 pathway.
Marta Wróbel +13 more
doaj +1 more source
Advanced Science, EarlyView.A microfluidic device with monolithically integrated nanostructures enables high‐throughput wounding of the cell membrane and nuclear envelope. The spatial distribution of charged multivesicular body protein 4B (CHMP4B) within the cell is mapped before and after wounding, showing involvement in repair of both the cell membrane and nuclear envelope ...
Apresio K. Fajrial +5 more
wiley +1 more source
Structural basis for endosomal recruitment of ESCRT‐I by ESCRT‐0 in yeast [PDF]
The EMBO Journal, 2011 The ESCRT-0 and ESCRT-I complexes coordinate the clustering of ubiquitinated cargo with intralumenal budding of the endosomal membrane, two essential steps in vacuolar/lysosomal protein sorting from yeast to humans. The 1.85-Å crystal structure of interacting regions of the yeast ESCRT-0 and ESCRT-I complexes reveals that PSDP motifs of the Vps27 ESCRT-
Xuefeng, Ren, James H, Hurley
openaire +2 more sources
Model of NiV-C interactions with ESCRT. [PDF]
, 2016 Vps28 acts as an adaptor between ESCRT-I and ESCRT-II. The NTD of Vps28 interacts with ESCRT-I via Tsg101-CTD, while the CTD of Vps28 interacts with ESCRT-II possibly via hVps36.
Tatyana Yun (404063) +8 more
core +1 more source
Advanced Intelligent Discovery, EarlyView.Exosomes are emerging as powerful biomarkers for disease diagnosis and monitoring. This review highlights the integration of surface‐enhanced Raman spectroscopy with artificial intelligence to enhance molecular fingerprinting of exosomes. Machine learning and deep learning techniques improve spectral interpretation, enabling accurate classification of ...
Munevver Akdeniz +2 more
wiley +1 more source