Results 61 to 70 of about 53,095 (341)
Functional classification of esterases from leaves of Aspidosperma polyneuron M. Arg. (Apocynaceae)
Genetics and Molecular Biology, 2003 Polyacrylamide gel electrophoresis system (PAGE) and inhibition tests for biochemical characterization of alpha- and beta-esterases were used to obtain a functional classification of esterases fromAspidosperma polyneuron.
Carvalho Vanda Marilza de +3 more
doaj
Structure-guided protein engineering increases enzymatic activities of the SGNH family esterases
Biotechnology for Biofuels, 2020 Background Esterases and lipases hydrolyze short-chain esters and long-chain triglycerides, respectively, and therefore play essential roles in the synthesis and decomposition of ester bonds in the pharmaceutical and food industries.
Zhengyang Li +12 more
doaj +1 more source
Stage‐Specific H3K14 and H3K23 Succinylation Orchestrates Insect Metamorphosis and Oogenesis
Advanced Science, EarlyView.Stage‐specific succinylation of histone H3 at lysine 14 and 23 differentially controls insect metamorphosis and oogenesis through distinct GPCR‐PKC signaling cascades, revealing an evolutionarily conserved epigenetic mechanism that coordinates key life‐history traits.
Yu‐Pu Jing +9 more
wiley +1 more source
Angewandte Chemie, EarlyView.We present a bioluminescent diagnostic platform that integrates DNA amplification and detection in a single step. The engineering of thermostable luciferase‐intercalating dye conjugates and controlled release of caged luciferin substrates enable real‐time, detection of attomolar concentrations of viral DNA within 30 min.
Yosta de Stigter +8 more
wiley +2 more sources
Advanced Science, EarlyView.A new generation of high‐precision fluorescent probes based on the lysosomal characteristics in tumor metabolic reprogramming and overexpressed tumor markers. The probe (CN‐D‐GGT) selectively label cancer cells in co‐culture systems and distinguish tumor lesions from inflamedand normal tissues in vivo, enabling precise tumor imaging.
Yang Shen +6 more
wiley +1 more source
Multicomponent Stapling of Glucagon‐Like Peptide‐1 Enables Receptor‐Guided PROTAC Delivery
Angewandte Chemie, EarlyView.We report a stapled glucagon‐like peptide‐1 (GLP‐1) analogue created via multicomponent tryptophan‐mediated Petasis reaction (TMPR). This strategy yields a stabilised peptide with superior helicity and improved potency. Conjugation to a bromodomain‐containing protein 4 (BRD4) degrader creates the first GLP‐1‐guided targeted protein degrader (PROTAC ...
Jan L. Venne +5 more
wiley +2 more sources
Frontiers in Microbiology, 2020 Construction of recombinant Escherichia coli strains carrying feruloyl esterase genes for secretory expression offers an attractive way to facilitate enzyme purification and one-step production of ferulic acid from agricultural waste.
Zhenshang Xu +7 more
doaj +1 more source
Confocal microscopy and image analysis indicates a region-specific relation between active caspases and cytoplasm in ejaculated and epididymal sperm. [PDF]
PLoS ONE, 2012 Previously, it was suggested a relation between the presence of apoptosis markers with cytoplasm in mammalian sperm. In this work, flow cytometry, confocal microscopy and image analysis were used to analyze the relationship between active caspase-3 and ...
Susana García Vazquez +2 more
doaj +1 more source
THE DISTRIBUTION OF ESTERASES IN MOUSE TISSUES
, 1959 The esterases contained in 32 mouse tissues and organs have been analyzed using the zymogram technique. More than ten different esterases have been resolved and partly characterized by their substrate and inhibition specificities.
CLEMENT L. MARKERT, ROBERT L. HUNTER
core +1 more source