Results 51 to 60 of about 604,841 (328)

Intrabody Expression in Eukaryotic Cells

, 2009
We describe procedures for intracellular expression of scFv in eukaryotic cells. Starting from a scFv gene cloned in a phage-display vector, we describe the cloning step into a mammalian expression vector, the transient transfection of a HeLa cell line, and the monitoring of intrabody expression by immunofluorescence staining and FACS analysis.
Guglielmi, Laurence, Martineau, Pierre
openaire   +4 more sources

From omics to AI—mapping the pathogenic pathways in type 2 diabetes

FEBS Letters, EarlyView.
Integrating multi‐omics data with AI‐based modelling (unsupervised and supervised machine learning) identify optimal patient clusters, informing AI‐driven accurate risk stratification. Digital twins simulate individual trajectories in real time, guiding precision medicine by matching patients to targeted therapies.
Siobhán O'Sullivan, Lu Qi, Pierre Zalloua   +2 more
wiley   +1 more source

Evolution: functional evolution of nuclear structure. [PDF]

, 2011
The evolution of the nucleus, the defining feature of eukaryotic cells, was long shrouded in speculation and mystery. There is now strong evidence that nuclear pore complexes (NPCs) and nuclear membranes coevolved with the endomembrane system, and that ...
Dawson, Scott C, Wilson, Katherine L
core  

The Unicellular State as a Point Source in a Quantum Biological System. [PDF]

, 2016
A point source is the central and most important point or place for any group of cohering phenomena. Evolutionary development presumes that biological processes are sequentially linked, but neither directed from, nor centralized within, any specific ...
Miller, William B, Torday, John S
core   +3 more sources

Biomembranal Myristoyl-Phosphatidylcholine as a Potential Target of the Cell Injury Activity of Vitamin D Decomposition Products in Eukaryotic Cells

Frontiers in Bioscience-Landmark
Background: Vitamin D decomposition products target a myristic acid sidechain of the predominant glycerophospholipid constructed in the biomembranes of Helicobacter pylori, causing gastric cancer in humans, and disrupt the membrane ...
Hirofumi Shimomura, Kiyofumi Wanibuchi, Kouich Hosoda, Avarzed Amgalanbaatar, Mitsuru Shoji, Shunji Hayashi   +5 more
doaj   +1 more source

Phagocytosis-like cell engulfment by a planctomycete bacterium

Nature Communications, 2019
Phagocytosis is a typically eukaryotic feature that could be behind the origin of eukaryotic cells. Here, the authors describe a bacterium that can engulf other bacteria and small eukaryotic cells through a phagocytosis-like mechanism.
Takashi Shiratori, Shigekatsu Suzuki, Yukako Kakizawa, Ken-ichiro Ishida   +3 more
doaj   +1 more source

A Bacterial Phage Tail-like Structure Kills Eukaryotic Cells by Injecting a Nuclease Effector

Cell Reports, 2019
Summary: Many bacteria interact with target organisms using syringe-like structures called contractile injection systems (CISs). CISs structurally resemble headless bacteriophages and share evolutionarily related proteins such as the tail tube, sheath ...
Iara Rocchi, Charles F. Ericson, Kyle E. Malter, Sahar Zargar, Fabian Eisenstein, Martin Pilhofer, Sinem Beyhan, Nicholas J. Shikuma   +7 more
doaj   +1 more source

Exploring lipid diversity and minimalism to define membrane requirements for synthetic cells

FEBS Letters, EarlyView.
Designing the lipid membrane of synthetic cells is a complex task, in which its various roles (among them solute transport, membrane protein support, and self‐replication) should all be integrated. In this review, we report the latest top‐down and bottom‐up advances and discuss compatibility and complexity issues of current engineering approaches ...
Sergiy Gan, Victoria Scarpelli, Marten Exterkate   +2 more
wiley   +1 more source

Protein trafficking through the endosomal system prepares intracellular parasites for a home invasion [PDF]

, 2013
Toxoplasma (toxoplasmosis) and Plasmodium (malaria) use unique secretory organelles for migration, cell invasion, manipulation of host cell functions, and cell egress. In particular, the apical secretory micronemes and rhoptries of apicomplexan parasites
A Fomovska, AE Topolska, B Lige, B Shen, BF Kafsack, BJ Luft, C Lord, CA Hunter, Chetan E. Chitnis, Christian Slomianny, CY He, D Richard, DL Alexander, F Parussini, H El Hajj, HC Hoppe, HC Hoppe, I Mellman, J Schrevel, JB Dacks, JC Boothroyd, JE Rothman, JF Dubremetz, JM Harper, K Kremer, K Miranda, KA Joiner, KA Joiner, KM Hager, L Pelletier, L Sheiner, LH Bannister, LH Bannister, LM Kats, M Elias, M Tufet-Bayona, M Yang, Markus Meissner, ME Francia, MH Ngo, MH Ngo, MK Shaw, MS Breinich, MS Marks, NA Counihan, PJ Bradley, PJ Sloves, S Mogelsvang, SD Brydges, Stanislas Tomavo, VB Carruthers, Vern B. Carruthers, WM Henne, X Que, Z Dou   +54 more
core   +2 more sources

Bacterial toxin effector membrane targeting: Outside in, then back again.

Frontiers in Cellular and Infection Microbiology, 2012
Pathogenic bacteria utilize multiple approaches to mediate their toxicity to eukaryotic cells. Dedicated protein machines deposit toxic effectors directly inside the host, whereas secreted toxins must enter cells independently of other bacterial ...
Brett eGeissler
doaj   +1 more source