Results 11 to 20 of about 106,156 (238)
Nature Communications, 2021 Eukaryotic DNA replication is mediated by many proteins which are tightly regulated for an efficient firing of replication at each cell cycle. Here the authors report a cryo-EM structure of the yeast ORC–Cdc6 bound to an 85-bp ARS1 origin DNA revealing ...
Xiang Feng +5 more
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Cell Reports, 2020 Summary: Eukaryotic DNA replication licensing is a prerequisite for, and plays a role in, regulating genome duplication that occurs exactly once per cell cycle.
Aftab Amin +10 more
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Coupling DNA Replication and Spindle Function in Saccharomyces cerevisiae
Cells, 2021 In the yeast Saccharomyces cerevisiae DNA replication and spindle assembly can overlap. Therefore, signaling mechanisms modulate spindle dynamics in order to ensure correct timing of chromosome segregation relative to genome duplication, especially when ...
Dimitris Liakopoulos
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Affinity Purification of an Archaeal DNA Replication Protein Network
mBio, 2010 Nineteen Thermococcus kodakarensis strains have been constructed, each of which synthesizes a different His6-tagged protein known or predicted to be a component of the archaeal DNA replication machinery.
Zhuo Li +4 more
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PLoS ONE, 2012 DNA replication initiates by formation of a pre-replication complex on sequences termed origins. In eukaryotes, the pre-replication complex is composed of the Origin Recognition Complex (ORC), Cdc6 and the MCM replicative helicase in conjunction with ...
Calvin Tiengwe +7 more
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Cell Reports, 2019 Summary: Progression of DNA replication depends on the ability of the replisome complex to overcome nucleoprotein barriers. During eukaryotic replication, the CMG helicase translocates along the leading-strand template and unwinds the DNA double helix ...
Hazal B. Kose +3 more
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TEX264 coordinates p97- and SPRTN-mediated resolution of topoisomerase 1-DNA adducts
Nature Communications, 2020 Eukaryotic topoisomerase 1 (TOP1) regulates DNA topology to ensure efficient DNA replication and transcription. Here, the authors reveal insights into the molecular resolution of topoisomerase 1-DNA adducts by TEX264, p97 and SPRTN.
John Fielden +16 more
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Global effects of DNA replication and DNA replication origin activity on eukaryotic gene expression
Molecular Systems Biology, 2009 This report provides a global view of how gene expression is affected by DNA replication. We analyzed synchronized cultures of Saccharomyces cerevisiae under conditions that prevent DNA replication initiation without delaying cell cycle progression.
Larsson Omberg +5 more
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RETRACTED: The structure of SV40 large T hexameric helicase in complex with AT-rich origin DNA
eLife, 2016 DNA replication is a fundamental biological process. The initial step in eukaryotic DNA replication is the assembly of the pre-initiation complex, including the formation of two head-to-head hexameric helicases around the replication origin.
Dahai Gai +3 more
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Structural Insight into the MCM double hexamer activation by Dbf4-Cdc7 kinase
Nature Communications, 2022 The Dbf4-dependent kinase Cdc7 (DDK) is essential for eukaryotic DNA replication. Here, the authors present a series of cryo-EM structures elucidating the versatility of this kinase in exerting an ordered phosphorylation of its essential target to ...
Jiaxuan Cheng +6 more
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