Ancient diversification of eukaryotic MCM DNA replication proteins
BMC Evolutionary Biology, 2009 Background Yeast and animal cells require six mini-chromosome maintenance proteins (Mcm2-7) for pre-replication complex formation, DNA replication initiation and DNA synthesis. These six individual MCM proteins form distinct heterogeneous subunits within
Aves Stephen J +2 more
doaj +1 more source
Damage-induced phosphorylation of Sld3 is important to block late origin firing. [PDF]
, 2010 Origins of replication are activated throughout the S phase of the cell cycle such that some origins fire early and others fire late to ensure that each chromosome is completely replicated in a timely fashion.
Defazio-Eli, Lisa G +5 more
core +2 more sources
TEX264 coordinates p97- and SPRTN-mediated resolution of topoisomerase 1-DNA adducts
Nature Communications, 2020 Eukaryotic topoisomerase 1 (TOP1) regulates DNA topology to ensure efficient DNA replication and transcription. Here, the authors reveal insights into the molecular resolution of topoisomerase 1-DNA adducts by TEX264, p97 and SPRTN.
John Fielden +16 more
doaj +1 more source
, 2019 Maintenance of genome integrity is a key process in all organisms. DNA polymerases (Pols) are central players in this process as they are in charge of the faithful reproduction of the genetic information, as well as of DNA repair.
De Veylder, Lieven +2 more
core +3 more sources
Molecular dissection of the domain architecture and catalytic activities of human PrimPol [PDF]
, 2014 PrimPol is a primase–polymerase involved in nuclear and mitochondrial DNA replication in eukaryotic cells. Although PrimPol is predicted to possess an archaeo-eukaryotic primase and a UL52-like zinc finger domain, the role of these domains has not been ...
Aidan J. Doherty +45 more
core +2 more sources
Global effects of DNA replication and DNA replication origin activity on eukaryotic gene expression
Molecular Systems Biology, 2009 This report provides a global view of how gene expression is affected by DNA replication. We analyzed synchronized cultures of Saccharomyces cerevisiae under conditions that prevent DNA replication initiation without delaying cell cycle progression.
Larsson Omberg +5 more
doaj +1 more source
RETRACTED: The structure of SV40 large T hexameric helicase in complex with AT-rich origin DNA
eLife, 2016 DNA replication is a fundamental biological process. The initial step in eukaryotic DNA replication is the assembly of the pre-initiation complex, including the formation of two head-to-head hexameric helicases around the replication origin.
Dahai Gai +3 more
doaj +1 more source
A quantitative model of the initiation of DNA replication in Saccharomyces cerevisiae predicts the effects of system perturbations. [PDF]
, 2012 BackgroundEukaryotic cell proliferation involves DNA replication, a tightly regulated process mediated by a multitude of protein factors. In budding yeast, the initiation of replication is facilitated by the heterohexameric origin recognition complex ...
DaSilva, Lance F +6 more
core +3 more sources
Structural Insight into the MCM double hexamer activation by Dbf4-Cdc7 kinase
Nature Communications, 2022 The Dbf4-dependent kinase Cdc7 (DDK) is essential for eukaryotic DNA replication. Here, the authors present a series of cryo-EM structures elucidating the versatility of this kinase in exerting an ordered phosphorylation of its essential target to ...
Jiaxuan Cheng +6 more
doaj +1 more source
Reconstitution of recombination-associated DNA synthesis with human proteins. [PDF]
, 2013 The repair of DNA breaks by homologous recombination is a high-fidelity process, necessary for the maintenance of genome integrity. Thus, DNA synthesis associated with recombinational repair must be largely error-free.
Grossi, Sara M +4 more
core +1 more source