Optimized Cas‐SF01 gene‐editing toolbox shortens flowering timing in commercial maize inbred JING724
The gene‐editing tool Cas‐SF01 was optimized to maximize its efficiency in maize. The Cas‐SF01‐TREX2 configuration was superior in enabling high‐purity gene mutations. This toolkit enabled commercial maize to flower seven days earlier without yield loss, thereby securing harvests and accelerating crop breeding.
Mengyuan Liu +11 more
wiley +1 more source
Os DNA sintéticos anti-sentido [PDF]
ANTISENSE SYNTHTETIC DNA. One old dream of the chemist in the field of the drug research is to create molecules capable of reaching their target with the precision of a missile.
Cravador, A.
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Enhancing CRISPR/Cas‐Mediated Gene Knockout With Short Non‐Homologous Oligonucleotides
ABSTRACT Chlamydomonas reinhardtii is a model green microalga that has great industrial potential as a sustainable bio‐factory for recombinant protein and high‐value chemical production. Efficient genome editing tools are required to redesign this organism for synthetic biology applications. CRISPR‐Cas editing technologies have already been adapted for
Yen Peng Chew +7 more
wiley +1 more source
Plant Peptides on the Rise: From Historical Insight to Future Applications
ABSTRACT Plant peptides constitute a rapidly expanding class of signalling molecules essential to plant physiology, mediating key processes such as development, stress adaptation, and immune responses. This review traces the history of plant peptide research, from the seminal discovery of systemin to the recent identification of non‐canonical peptides (
Shunxi Wang +5 more
wiley +1 more source
The INO80 chromatin remodeller facilitates DNA damage bypass via postreplicative gap repair
Chromatin remodellers play a crucial role in transcription, replication and genome maintenance by dynamically regulating the accessibility of DNA. The INO80 complex contributes to multiple aspects of DNA repair and DNA replication stress management.
Ronald P Wong +3 more
doaj +1 more source
Circular RNAs in Lotus japonicus Responses to Nutrient Supply and Mesorhizobium Symbiosis
ABSTRACT Symbiotic interactions between legumes and rhizobia enable nitrogen fixation under low nutrient conditions. The establishment and function of symbiotic interactions require coordinated changes in gene expression in both the host and the microbe. Circular RNAs (circRNAs) are endogenous gene‐specific molecules that can regulate transcription and
Delecia Utley +3 more
wiley +1 more source
Arabidopsis DXO1‐ and RNMT1‐Mediated m7G Capping of mRNA Modulates ABA Response
ABSTRACT Eukaryotic mRNA carries an N7‐methylguanosine (m⁷G) 5′ cap that is essential for mRNA stability and function. Although traditionally viewed as a constitutive process, m⁷G capping is increasingly recognized as dynamically regulated to control gene expression in response to environmental stimuli.
Shumin Liang +10 more
wiley +1 more source
Circular RNAs: Unlocking new avenues in cardiometabolic disease management
Abstract figure legend In the heart circular RNAs (circRNAs) function as microRNA sponges, interact with proteins and can even undergo translation. Advances in bioinformatics now enable their identification through high‐throughput RNA sequencing, whereas computational analyses reveal differential expression in cardiac disease settings.
Kimberley M. Mellor +4 more
wiley +1 more source
Advances in large DNA fragment assembly for microbial cell factory engineering
Abstract The efficient, rapid, and reliable assembly of DNA fragments is essential for advancing metabolic engineering and synthetic biology. With the rapid advancement of DNA synthesis and assembly technologies, the scale of DNA assembly has expanded from single genes to metabolic pathways and even genomes.
Yu Zhang +5 more
wiley +1 more source
Structural and functional analysis of RecJ-type exonucleases [PDF]
This research was originally published in The Journal of Biological Chemistry. Taisuke Wakamatsu, Yoshiaki Kitamura, Yutaro Kotera, Noriko Nakagawa, Seiki Kuramitsu, and Ryoji Masui.
ワカマツ, タイスケ +1 more
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