Cell & Bioscience, 2021 Background Coronavirus (CoV) nonstructural protein 14 (nsp14) has exoribonuclease (ExoN) activity, responsible for proofreading and contributing to replication fidelity. It has been reported that CoVs exhibit variable sensitivity to nsp14-ExoN deficiency.
Xiaoyu Niu +3 more
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Crystal structures and fragment screening of SARS-CoV-2 NSP14 reveal details of exoribonuclease activation and mRNA capping and provide starting points for antiviral drug development [PDF]
bioRxiv, 2022 The SARS-CoV-2 non-structural protein 14 (NSP14) is a dual function enzyme containing an N-terminal exonuclease domain (ExoN) and C-terminal Guanine-N7-methyltransferase (N7-MTase) domain.
Nergis Imprachim +2 more
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Degradation of microRNAs by a Family of Exoribonucleases in Arabidopsis [PDF]
Science, 2008 microRNAs (miRNAs) play crucial roles in numerous developmental and metabolic processes in plants and animals. The steady-state levels of miRNAs need to be properly controlled to ensure normal development. Whereas the framework of miRNA biogenesis is established, factors involved in miRNA degradation remain unknown.
Vanitharani Ramachandran, Xuemei Chen
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Xrn1 Exoribonuclease—An Intrinsic Marker of Yeast Population Growth
Frontiers in Bioscience-EliteBackground: Xrn1 exoribonuclease is the major mRNA degradation enzyme in Saccharomyces cerevisiae. In exponentially growing cells, Xrn1 is localised in the yeast cells and directs the degradation of mRNA molecules.
Tomas Grousl, Tomas Vomastek
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Substrate Recognition and Catalysis by the Exoribonuclease RNase R [PDF]
Journal of Biological Chemistry, 2006 RNase R is a processive, 3' to 5' hydrolytic exoribonuclease that together with polynucleotide phosphorylase plays an important role in the degradation of structured RNAs. However, RNase R differs from other exoribonucleases in that it can by itself degrade RNAs with extensive secondary structure provided that a single-stranded 3' overhang is present ...
Helen A. Vincent, Murray P. Deutscher
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Murine Hepatitis Virus Exoribonuclease nsp14 Is Required for the Biogenesis of Viral Circular RNAs
Microbiology Spectrum, 2023 Circular RNA (circRNA), a newly identified important component of the transcriptome, is formed by covalently bonded single-stranded RNA through back splicing (1) or other unknown mechanisms.
Shaomin Yang +8 more
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The human HELQ helicase and XRN2 exoribonuclease cooperate in R-loop resolution. [PDF]
Open BiolPan JM +5 more
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Crystallographic Studies of Bacterial Exoribonucleases [PDF]
The Scientific World JOURNAL, 2002 INTRODUCTION. Ribonucleases (RNases) play a central role in all cellular RNA processes. These processes include mRNA degradation, and maturation and turnover of stable RNAs, which are vital for the proper functioning of all cells. E. coli has served as a model system for understanding the role of ribonucleases in RNA metabolism, and eight distinct ...
Tristan J. Fiedler, Arun Malhotra
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The coronavirus nsp14 exoribonuclease interface with the cofactor nsp10 is essential for efficient virus replication and enzymatic activity. [PDF]
J VirolGrimes SL +8 more
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Exoribonuclease superfamilies: structural analysis and phylogenetic distribution
Nucleic Acids Research, 2001 Exoribonucleases play an important role in all aspects of RNA metabolism. Biochemical and genetic analyses in recent years have identified many new RNases and it is now clear that a single cell can contain multiple enzymes of this class. Here, we analyze the structure and phylogenetic distribution of the known exoribonucleases.
Y. Zuo
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