Results 11 to 20 of about 14,418 (193)

Tick-borne flavivirus exoribonuclease-resistant RNAs contain a double loop structure [PDF]

Nature Communications
Viruses from the Flaviviridae family contain human relevant pathogens that generate subgenomic noncoding RNAs during infection using structured exoribonuclease resistant RNAs (xrRNAs).
Conner J. Langeberg, Matthew J. Szucs, Madeline E. Sherlock, Quentin Vicens, Jeffrey S. Kieft   +4 more
doaj   +3 more sources

Mechanism and structural diversity of exoribonuclease-resistant RNA structures in flaviviral RNAs [PDF]

Nature Communications, 2018
Subgenomic flavivirus RNAs are generated by a host exoribonuclease and play an important role in virus replication and pathogenesis. Here, the authors show the mechanism by which subgenomic flavivirus RNAs are generated and identify two structurally ...
Andrea MacFadden, Zoe O’Donoghue, Patricia A. G. C. Silva, Erich G. Chapman, René C. Olsthoorn, Mark G. Sterken, Gorben P. Pijlman, Peter J. Bredenbeek, Jeffrey S. Kieft   +8 more
doaj   +2 more sources

RPA transforms RNase H1 to a bidirectional exoribonuclease for processive RNA–DNA hybrid cleavage

Nature Communications
RNase H1 has been acknowledged as an endoribonuclease specializing in the internal degradation of the RNA moiety within RNA–DNA hybrids, and its ribonuclease activity is indispensable in multifaceted aspects of nucleic acid metabolism.
Yanan Li, Chao Liu, Xinshuo Jia, Lulu Bi, Zhiyun Ren, Yilin Zhao, Xia Zhang, Lijuan Guo, Yanling Bao, Cong Liu, Wei Li, Bo Sun   +11 more
doaj   +2 more sources

Dis3l2 is essential for neural crest survival by modulating Akt signaling [PDF]

Cell Communication and Signaling
DIS3-like 3’-5’ exoribonuclease 2 (DIS3L2), an exoribonuclease, is known to preferentially degrade uridylated RNA substrates, miRNAs, and ncRNAs. Recent reports show that DIS3L2 also plays a key role in cell proliferation and tumor growth.
Sian D’Silva, Tuhina Prasad, Megha Kumar   +2 more
doaj   +2 more sources

Discoveries of Exoribonuclease-Resistant Structures of Insect-Specific Flaviviruses Isolated in Zambia

Viruses, 2020
To monitor the arthropod-borne virus transmission in mosquitoes, we have attempted both to detect and isolate viruses from 3304 wild-caught female mosquitoes in the Livingstone (Southern Province) and Mongu (Western Province) regions in Zambia in 2017. A
Christida E. Wastika, Hayato Harima, Michihito Sasaki, Bernard M. Hang’ombe, Yuki Eshita, Yongjin Qiu, William W. Hall, Michael T. Wolfinger, Hirofumi Sawa, Yasuko Orba   +9 more
doaj   +2 more sources

Shared properties and singularities of exoribonuclease-resistant RNAs in viruses

Computational and Structural Biotechnology Journal, 2021
What viral RNA genomes lack in size, they make up for in intricacy. Elaborate RNA structures embedded in viral genomes can hijack essential cellular mechanisms aiding virus propagation.
Quentin Vicens, Jeffrey S. Kieft
doaj   +3 more sources

Alterations of the transcriptome of Sulfolobus acidocaldarius by exoribonuclease aCPSF2.

PLoS ONE, 2013
Recent studies identified a 5´ to 3´ exoribonuclease termed Sso-RNase J in the crenarchaeon Sulfolobus solfataricus (Sso), which has been reclassified to the aCPSF2 (archaeal cleavage and polyadenylation specificity factor 2) group of β-CASP proteins. In
Birgit Märtens, Fabian Amman, Salim Manoharadas, Lukas Zeichen, Alvaro Orell, Sonja-Verena Albers, Ivo Hofacker, Udo Bläsi   +7 more
doaj   +8 more sources

Xrn1 Exoribonuclease—An Intrinsic Marker of Yeast Population Growth

Frontiers in Bioscience-Elite
Background: Xrn1 exoribonuclease is the major mRNA degradation enzyme in Saccharomyces cerevisiae. In exponentially growing cells, Xrn1 is localised in the yeast cells and directs the degradation of mRNA molecules.
Tomas Grousl, Tomas Vomastek
doaj   +3 more sources

Heterozygous loss-of-function alleles associate the conserved 3'-5' exoribonuclease EXOSC10 with hypersensitivity to the anticancer drug 5-fluorouracil. [PDF]

Mol Oncol
EXOSC10, an essential nuclear RNA exosome‐associated 3′‐5′ exoribonuclease, is inhibited by the anticancer drug 5‐fluorouracil (5‐FU), and EXOSC10 depletion increases 5‐FU sensitivity. The colon‐cancer variant EXOSC10S402T, located in a proteolysis motif, is stable and nuclear but nonfunctional in vivo.
Sain R, Le Page Y, Percevault F, Becker E, Negroni L, Fernandez A, Cantero M, Muñoz-Santos D, Montoliu L, Garnier C, Primig M.   +10 more
europepmc   +2 more sources

Computational Characterizations of the Interactions Between the Pontacyl Violet 6R and Exoribonuclease as a Potential Drug Target Against SARS-CoV-2

Frontiers in Chemistry, 2021
We report a molecular-docking and virtual-screening-based identification and characterization of interactions of lead molecules with exoribonuclease (ExoN) enzyme in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). From previously identified
Rangika Munaweera, Ying S. Hu
doaj   +1 more source