Results 41 to 50 of about 14,025 (272)

Elastic Coupling Between RNA Degradation and Unwinding by an Exoribonuclease [PDF]

Science, 2012
Loading a Spring To regulate cellular RNA levels, transcription must be balanced by RNA degradation. An important player is the exosome, which can unwind and degrade structured RNA. Lee et al. (p.
Gwangrog Lee, Matthew Bratkowski, Fang Ding, Ailong Ke, Taekjip Ha   +4 more
openalex   +4 more sources

HELZ2: a new, interferon-regulated, human 3′-5′ exoribonuclease of the RNB family is expressed from a non-canonical initiation codon

bioRxiv, 2023
Proteins containing a RNB domain, originally identified in E. coli RNase II, are widely present throughout the tree of life. Many RNB proteins are endowed with 3’-5’ exoribonucleolytic activity but some have lost catalytic function during evolution ...
E. Huntzinger, Jordan Sinteff, Bastien Morlet, B. Séraphin   +3 more
semanticscholar   +1 more source

RNA-binding proteins and exoribonucleases modulating miRNA in cancer: the enemy within. [PDF]

Exp Mol Med
Seo Y, Rhim J, Kim JH.
europepmc   +2 more sources

The coronavirus proofreading exoribonuclease mediates extensive viral recombination

bioRxiv, 2020
Coronaviruses (CoVs) emerge as zoonoses and cause severe disease in humans, demonstrated by the SARS-CoV-2 (COVID-19) pandemic. RNA recombination is required during normal CoV replication for subgenomic mRNA (sgmRNA) synthesis and generates defective ...
J. Gribble, A. Pruijssers, Maria L. Agostini, J. Anderson-Daniels, J. Chappell, Xiaotao Lu, Laura J. Stevens, Andrew L. Routh, M. Denison   +8 more
semanticscholar   +1 more source

Interfering with nucleotide excision by the coronavirus 3′-to-5′ exoribonuclease

bioRxiv, 2022
Some of the most efficacious antiviral therapeutics are ribonucleos(t)ide analogs. The presence of a 3’-to-5’ proofreading exoribonuclease (ExoN) in coronaviruses diminishes the potency of many ribonucleotide analogs.
Rukesh Chinthapatla, M. Sotoudegan, Pankaj Srivastava, T. K. Anderson, I. Moustafa, Kellan T. Passow, Samantha A. Kennelly, R. Moorthy, David Dulin, Joy Y. Feng, Daniel A. Harki, R. N. Kirchdoerfer, C. Cameron, J. Arnold   +13 more
semanticscholar   +1 more source

The Enzymatic Activity of the nsp14 Exoribonuclease Is Critical for Replication of MERS-CoV and SARS-CoV-2

Journal of Virology, 2020
The bifunctional nsp14 subunit of the coronavirus replicase contains 3′-to-5′ exoribonuclease (ExoN) and guanine-N7-methyltransferase domains. For the betacoronaviruses MHV and SARS-CoV, ExoN was reported to promote the fidelity of genome replication ...
N. Ogando, Jessika C. Zevenhoven-Dobbe, Y. van der Meer, P. Bredenbeek, C. Posthuma, E. Snijder   +5 more
semanticscholar   +1 more source

Exoribonucleases as Modulators of Virulence in Pathogenic Bacteria [PDF]

Frontiers in Cellular and Infection Microbiology, 2012
Pathogenic bacteria are responsible for severe diseases worldwide. RNA stability is a major player controlling the expression of virulence factors. Ribonucleases (RNases) are the enzymes responsible for the maturation and degradation of RNA molecules (Arraiano et al., 2010; Silva et al., 2011).
José M. Andrade, Cecília M. Arraiano, Vânia Pobre, Cátia Bárria, Rute G. Matos   +4 more
openaire   +4 more sources

Heterozygous PNPT1 Variants Cause a Sensory Ataxic Neuropathy. [PDF]

Eur J Neurol
ABSTRACT Background Biallelic variants in polyribonucleotide‐nucleotidyltransferase‐1 (PNPT1) have been associated with a range of phenotypes from syndromic hearing loss to Leigh's syndrome. More recently, heterozygous variants in PNPT1, have been reported in three families with cerebellar ataxia and prominent sensory neuropathy.
Haddad S, Record CJ, Self E, Skorupinska M, Rossor AM, Laura M, Ingle G, Manzur A, Muntoni F, Blake JC, Reilly MM.   +10 more
europepmc   +2 more sources

Real-time fluorescence detection of exoribonucleases [PDF]

RNA, 2009
The identification of RNases or RNase effectors is a continuous challenge, particularly given the current importance of RNAs in the control of genome expression. Here, we show that a fluorogenic RNA–DNA hybrid is a powerful tool for a real-time fluorescence detection and assay of exoribonucleases (RT-FeDEx).
Sinturel, F., Pellegrini, O., Xiang, S., Tong, L., Condon, C., Bénard, L.   +5 more
openaire   +4 more sources

Pseudoknot length modulates the folding, conformational dynamics, and robustness of Xrn1 resistance of flaviviral xrRNAs

Nature Communications, 2021
Exoribonuclease-resistant RNAs (xrRNAs) are RNA elements that block the exoribonucleolytic degradation of RNA. Here the authors show how a long-range pseudoknot length modulates the Mg2+-dependence of flaviviral xrRNA’s folding, conformational dynamics ...
Xiaolin Niu, Ruirui Sun, Zhifeng Chen, Yirong Yao, Xiaobing Zuo, Chunlai Chen, Xianyang Fang   +6 more
doaj   +1 more source