Results 101 to 110 of about 3,039 (115)

5′-3′ Exoribonucleases

2011
The 5′-3′ exoribonucleases have important functions in RNA processing, RNA degradation, RNA interference, transcription, and other cellular processes. The Xrn1 and Xrn2/Rat1 family of enzymes are the best characterized 5′-3′ exoribonucleases, and there has been significant recent progress in the understanding of their structure and function. Especially,
Jeong Ho Chang, Song Xiang, Liang Tong
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5′-Exoribonuclease 1: Xrn1

2001
Publisher Summary The detection, purification, and partial characterization of 5′-exoribonuclease 1 (Xrn1) from Saccharomyces cerevisiae as a 160-kDa RNase was reported in 1980 and 1985. The enzyme is a processive exonuclease hydrolyzing RNA from the 5′ end with the production of 5′-mononucleofides.
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A cytoplasmic exoribonuclease from HeLa cells

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1977
An exoribonuclease has been purified from the cytoplasm of HeLa cells. The enzyme produces 5'-AMP as the only product from poly(A). The degradation proceeds in a 3' to 5' direction, and a 3'-OH terminus is required. In addition to poly(A), the enzyme degrades other synthetic homopolymers as well as natural messenger, and ribosomal RNAs.
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An exoribonuclease in bovine brain

Brain Research, 1975
An exonuclease which degrades RNA to yield 3′-mononucleotides has been purified 300-fold from an acetone powder of bovine brain white matter. The enzyme preparation does not contain detectable quantities of RNase A, DNase, 2′,3′-cyclic phosphodiesterase, 5′-nucleotidase, and acid and alkaline phosphatase activities and requires sulfhydryl compounds for
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Structural basis of exoribonuclease-mediated mRNA transcription termination

Nature
Efficient termination is required for robust gene transcription. Eukaryotic organisms use a conserved exoribonuclease-mediated mechanism to terminate the mRNA transcription by RNA polymerase II (Pol II)1-5. Here we report two cryogenic electron microscopy structures of Saccharomyces cerevisiae Pol II pre-termination transcription complexes bound to the
Yuan Zeng, Hong-Wei Zhang, Xiao-Xian Wu, Yu Zhang   +3 more
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Exoribonucleases and their multiple roles in RNA metabolism

2000
In recent years there has been a dramatic shift in our thinking about ribonucleases (RNases). Although they were once considered to be nonspecific, degradative enzymes, it is now clear that RNases play a central role in every aspect of cellular RNA metabolism, including decay of mRNA, conversion of RNA precursors to their mature forms, and end-turnover
M P, Deutscher, Z, Li
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Exoribonuclease Activity of Purified Reverse Transcriptase Preparations from Retroviruses

The Journal of Biochemistry, 1989
Highly purified and commercially available preparations of reverse transcriptases from retroviruses contain a 3' to 5' exoribonuclease activity capable of hydrolyzing synthetic homopolyribonucleotides having a 3'-OH end. The exoribonuclease activity of reverse transcriptase preparations from Rous associated virus-2 was further characterized.
Y, Kikuchi, Y, Ando, N, Ichimura, A, Noda   +3 more
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Studying Exoribonuclease Activity Using Fluorescence Anisotropy Assay

Fluorescence anisotropy is a powerful technique, widely used for investigating ligand-macromolecule binding and high-throughput screens for drugs. Here, we employ fluorescence anisotropy to quantitatively study the activity of exoribonucleases exemplified by the Xrn2 enzyme.
Krzysztof, Kuś, Lidia, Vasiljeva
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Cooperation of Endo- and Exoribonucleases in Chloroplast mRNA Turnover

2004
Chloroplasts were acquired by eukaryotic cells through endosymbiosis and have retained their own gene expression machinery. One hallmark of chloroplast gene regulation is the predominance of posttranscriptional control, which is exerted both at the gene-specific and global levels.
Thomas J, Bollenbach, Gadi, Schuster, David B, Stern   +2 more
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Function and Characterization of Poly(A)-Specific 3´ Exoribonucleases

1997
Poly(A) tails are commonly found at the 3´ end of various classes of RNA (reviewed in Brawerman 1981; Manley 1995b). They are evolutionarily widespread and appear on RNAs of several different organisms. In mammalian cells almost all mRNAs end with an approximately 200-adenosine-residue-long poly(A) tail.
A, Virtanen, J, Aström
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