Reconstitution of the complete pathway of ITS2 processing at the pre-ribosome
, 2017 Removal of internal transcribed spacer 2 (ITS2) from pre-ribosomal RNA is essential to make functional ribosomes. This complicated processing reaction begins with a single endonucleolytic cleavage followed by exonucleolytic trimming at both new cleavage ...
Conti, E. +6 more
core +2 more sources
Mutations in Mtr4 Structural Domains Reveal Their Important Role in Regulating tRNA\u3csub\u3ei\u3c/sub\u3e \u3csup\u3eMet\u3c/sup\u3e Turnover in \u3cem\u3eSaccharomyces cerevisiae\u3c/em\u3e and Mtr4p Enzymatic Activities \u3cem\u3eIn Vitro\u3c/em\u3e [PDF]
, 2016 RNA processing and turnover play important roles in the maturation, metabolism and quality control of a large variety of RNAs thereby contributing to gene expression and cellular health.
Anderson, James T. +2 more
core +1 more source
Engineering of a conditional allele reveals multiple roles of XRN2 in Caenorhabditis elegans development and substrate specificity in microRNA turnover [PDF]
, 2017 Although XRN2 proteins are highly conserved eukaryotic 5′→3′ exonucleases, little is known about their function in animals. Here, we characterize Caenorhabditis elegans XRN2, which we find to be a broadly and constitutively expressed nuclear protein.
Gaidatzis, Dimos +4 more
core
Nuclear Surveillance and Degradation of Hypomodified Initiator tRNA\u3csup\u3eMet\u3c/sup\u3e in \u3cem\u3eS. cerevisiae\u3c/em\u3e [PDF]
, 2004 The tRNA m1A58 methyltransferase is composed of two subunits encoded by the essential genes TRM6 and TRM61 (formerly GCD10 and GCD14). The trm6-504 mutation results in a defective m1A methyltransferase (Mtase) and a temperature-sensitive growth phenotype
Anderson, James T. +5 more
core +1 more source
Xrn1 Exoribonuclease—An Intrinsic Marker of Yeast Population Growth
Frontiers in Bioscience-EliteBackground: Xrn1 exoribonuclease is the major mRNA degradation enzyme in Saccharomyces cerevisiae. In exponentially growing cells, Xrn1 is localised in the yeast cells and directs the degradation of mRNA molecules. Xrn1 is gradually deposited and presumably inactivated in the processing bodies (P-bodies) as the yeast population ages.
Tomas Vomastek, Tomas Grousl
openaire +3 more sources
Processing of preribosomal RNA in Saccharomyces cerevisiae [PDF]
, 2015 Most, if not all RNAs, are transcribed as precursors that require processing to gain functionality. Ribosomal RNAs (rRNA) from all organisms undergo both exo- and endonucleolytic processing.
Cruz, Jesús de la +2 more
core
The role of RNA Polymerase II-dependent transcription elongation in the cross-talk between mRNA synthesis and decay. [PDF]
, 2018 The main molecule in gene expression is messenger RNA (mRNA) which transfers the information contained in genes in the nucleus to the cytoplasm where it is translated into proteins that carry out cellular functions. mRNA levels are determined through its
Begley, Victoria Sarah
core
Uniquely conserved immunosuppressive viral exoribonucleases
Oncotarget, 2017 Meyer, Bjoern, Ly, Hinh
openaire +2 more sources
Exoribonuclease-resistant RNAs in tick-borne flaviviruses
Tick-borne flaviviruses (TBF) are a group of emerging and re-emerging pathogens that affect large geographic regions, including Europe, Asia, North America, and Africa, posing a major public health threat. A critical aspect of their molecular biology and pathology is the presence of evolutionarily conserved functional RNAs in their 3’ untranslated ...
Sidl, Leonhard +3 more
openaire +1 more source
Bacillus subtilis NrnB is expressed during sporulation and acts as a unique 3'-5' exonuclease. [PDF]
Nucleic Acids Res, 2023 Myers TM +6 more
europepmc +1 more source