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The earthworm Expressed Sequence Tag project
Pedobiologia, 2003This paper aims to provide a brief description of the earthworm Expressed Sequence Tag (EST) project. ESTs are short single read sequences randomly derived from cDNA libraries and provide the means to acquire large scale sequence information of coding DNA.
Sturzenbaum, S R +5 more
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Obtaining Accurate Translations from Expressed Sequence Tags
2009The genomes of an increasing number of species are being investigated through the generation of expressed sequence tags (ESTs). However, ESTs are prone to sequencing errors and typically define incomplete transcripts, making downstream annotation difficult. Annotation would be greatly improved with robust polypeptide translations.
James, Wasmuth, Mark, Blaxter
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Complementary DNA Sequencing: Expressed Sequence Tags and Human Genome Project
Science, 1991Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences.
Mark D. Adams +12 more
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ANALYSIS OF GRAPE EXPRESSED SEQUENCES TAGS
Acta Horticulturae, 2000The Australian Agricultural Research Institute is embarking on a large-scale grape EST analysis project. cDNA libraries are being prepared from a range of tissues, developmental stages and cultivars with the aim of producing a database of up to 50,000 sequences, covering a high proportion of grape genes.
E.M. Ablett, L.S. Lee, R.J. Henry
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Global Assembly of Expressed Sequence Tags
2012The method for the construction of Expressed Sequence Tag (EST) assemblies described here uses reads generated from 454 pyrosequencing and Sanger and Illumina (Solexa) sequencing technologies as input. It is consistent with and parallels many established EST assembly protocols, for example the TIGR Gene Indices.
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Toxoplasma gondii expressed sequence tags: insight into tachyzoite gene expression
Molecular and Biochemical Parasitology, 1996Analysis of DNA sequences from the 5' end of 239 directionally cloned Toxoplasma gondii RH strain tachyzoite-derived cDNAs revealed significant similarity to several classes of genes/proteins including 24 ribosomal proteins, five metabolic enzymes, four cell-cycle regulators and 15 previously cloned T. gondii genes.
K L, Wan, J M, Blackwell, J W, Ajioka
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Discovering conserved insect microRNAs from expressed sequence tags
Journal of Insect Physiology, 2010MicroRNAs (miRNA) participate in regulating diverse biological pathways by translational repression in animals. They have attracted increasing attention recently. However, little work has been done on the miRNA genes in agriculturally important pests. Because the transcripts of most miRNA genes are the products of type-II RNA polymerase, pri-miRNA has ...
Qidong, Jia +4 more
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Expressed sequence tags: medium-throughput protocols.
Methods in molecular biology (Clifton, N.J.), 2004Generating expressed sequence tags is a simple, cheap, and efficient way to sample the genome of a target organism. An expressed sequence tag (EST) is a single-pass sequence derived from a single complementary DNA (cDNA) clone, and the sequence serves to identify the gene from which it derives.
Claire, Whitton +3 more
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Expressed sequence tags: analysis and annotation.
Methods in molecular biology (Clifton, N.J.), 2004Expressed sequence tags (ESTs) present a special set of problems for bioinformatic analysis. They are partial and error-prone, and large datasets can have significant internal redundancy. To facilitate analysis of small EST datasets from in-house projects, we present an integrated "pipeline" of tools that take EST data from sequence trace to database ...
John, Parkinson, Mark, Blaxter
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