Results 1 to 10 of about 2,321,627 (280)

Nanoparticles Affect the Expression Stability of Housekeeping Genes in Plant Cells [PDF]

Nanotechnology, Science and Applications, 2020
Rajendran K Selvakesavan, Gregory Franklin Institute of Plant Genetics of the Polish Academy of Sciences, Poznan 60-479, PolandCorrespondence: Gregory Franklin Tel +48 61 655 0266Email fgre@igr.poznan.plPurpose: We report on the expression stability of ...
Selvakesavan RK, Franklin G
doaj   +2 more sources

Stable Reference Gene Selection for qRT-PCR Normalization in Strawberry (Fragaria × ananassa) Leaves under Different Stress and Light-Quality Conditions

Horticulturae, 2021
Selecting an appropriate reference gene is of crucial importance for improving the accuracy of qRT-PCR analyses. In this study, strawberry (Fragaria ananassa) seedlings were subjected to different environmental conditions including heat, cold, drought ...
Yuntian Ye, Yang Lu, Guangyi Wang, Yongqiang Liu, Yunting Zhang, Haoru Tang   +5 more
doaj   +1 more source

Assessment of Suitable Reference Genes for qRT-PCR Normalization in Eocanthecona furcellata (Wolff)

Insects, 2022
Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) is a widely used tool for measuring gene expression; however, its accuracy relies on normalizing the data to one or more stable reference genes.
Ying-Na Pan, Ru-Na Zhao, Di Fu, Chun Yu, Chun-Ni Pan, Wei Zhou, Wen-Long Chen   +6 more
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Reference Genes for Expression Analysis Using RT-qPCR in Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)

Insects, 2022
Cnaphalocrocis medinalis is a destructive migratory rice pest. Although many studies have investigated its behavioral and physiological responses to environmental changes and migration-inducing factors, little is known about its molecular mechanisms ...
Xiaoyu Zhao, Jiawen Guo, Yanhui Lu, Tianyi Sun, Junce Tian, Jianlei Huang, Hongxing Xu, Zhengliang Wang, Zhongxian Lu   +8 more
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Effective Alu repeat based RT-qPCR normalization in cancer cell perturbation experiments [PDF]

, 2013
Background: Measuring messenger RNA (mRNA) levels using the reverse transcription quantitative polymerase chain reaction (RT-qPCR) is common practice in many laboratories.
Beckers, Anneleen, De Brouwer, Sara, Kumps, Candy, Leonelli, Carina, Mestdagh, Pieter, Mets, Evelien, Pattyn, Filip, Rihani, Ali, Rondou, Pieter, Speleman, Franki, Van der Meulen, Joni, Van Maerken, Tom, Van Peer, Gert, Vandesompele, Jo   +13 more
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Validation of Potential Reference Genes for Real-Time qPCR Analysis in Pharaoh Ant, Monomorium pharaonis (Hymenoptera: Formicidae)

Frontiers in Physiology, 2022
Ants are highly diverse social insects living in colonies consisted of up to millions of individuals with reproductive division of labors. Due to the interests in disclosing the genetic and epigenetic regulation mechanisms underlying the distinct ...
Guo Ding, Guo Ding, Guo Ding, Qionghua Gao, Qionghua Gao, Jun Chen, Jie Zhao, Guojie Zhang, Guojie Zhang, Guojie Zhang, Weiwei Liu   +10 more
doaj   +1 more source

Selection and Validation of Reference Genes for qRT-PCR Analysis of Gene Expression in Tropaeolum majus (Nasturtium)

Horticulturae, 2023
Tropaeolum majus (nasturtium) is an important ornamental and medicinal plant due to its colorful flowers, shield-shaped leaves, and richness in mineral elements and bioactive compounds. However, the key genes related to these important biological traits,
Qing Tang, Guang-Can Zhou, Si-Jie Liu, Wen Li, Yi-Lei Wang, Gao-Ying Xu, Teng-Fei Li, Guo-Qing Meng, Jia-Yu Xue   +8 more
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Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in the Bean Bug, Riptortus pedestris (Hemiptera: Alydidae)

Insects, 2023
Quantitative real-time PCR (qRT-PCR) is widely accepted as a precise and convenient method for quantitatively analyzing the expression of functional genes. The data normalization strongly depends upon stable reference genes.
Liuyang Wang, Qingyu Liu, Pei Guo, Zhanlin Gao, Dan Chen, Tao Zhang, Jun Ning   +6 more
doaj   +1 more source

Transcriptional search to identify and assess reference genes for expression analysis in Solanumlycopersicum under stress and hormone treatment conditions

Journal of Integrative Agriculture, 2022
Tomato (Solanumlycopersicum) is a model plant for research on fruit development and stress response, in which gene expression analysis is frequently conducted.
Yao-ke DUAN, Rong HAN, Yan SU, Ai-ying WANG, Shuang LI, Hao SUN, Hai-jun GONG   +6 more
doaj   +1 more source

Hprt Serves as an Ideal Reference Gene for qRT-PCR Normalization in Rat DRG Neurons

Journal of Integrative Neuroscience, 2023
Objective: To identify suitable reference genes for gene expression studies in rat dorsal root ganglia (DRG) neurons. Methods: The raw cycle threshold (Ct) values of 12 selected reference genes were obtained via quantitative real-time reverse ...
Fenglan Liu, Yang Miao, Yaxian Wang, Qianqian Shan   +3 more
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