Results 11 to 20 of about 2,321,627 (280)

Analytic expression for Taylor-Couette stability boundary [PDF]

Physics of Fluids, 1996
We analyze the mechanism that determines the boundary of stability in Taylor-Couette flow. By simple physical argument we derive an analytic expression to approximate the stability line for all radius ratios and all speed ratios, for co- and ...
Alexander Esser, Couette M. M., Mallock A., Siegfried Grossmann, Tagg R.   +4 more
core   +2 more sources

Improving Protein Expression, Stability, and Function with ProteinMPNN. [PDF]

J Am Chem Soc, 2023
AbstractNatural proteins are highly optimized for function, but are often difficult to produce at a scale suitable for biotechnological applications due to poor expression in heterologous systems, limited solubility, and sensitivity to temperature.
Sumida KH, Núñez-Franco R, Kalvet I, Pellock SJ, Wicky BIM, Milles LF, Dauparas J, Wang J, Kipnis Y, Jameson N, Kang A, De La Cruz J, Sankaran B, Bera AK, Jiménez-Osés G, Baker D.   +15 more
europepmc   +6 more sources

Genetic Stability, Inheritance Patterns and Expression Stability in Biotech Crops [PDF]

OBM Genetics, 2020
Demonstration of the stability of traits newly introduced into a plant genome via genetic engineering approaches comprise a significant portion of the safety assessment that these products undergo prior to receiving the requisite regulatory approvals enabling commercial authorization.
Laura Privalle, Patricia Back, Apurv Bhargava, Zach Bishop, Krystal Cisneros, Isabelle Coats, Ine Criel, Lien Dhondt, Travis Draughn, Barb Fowler, Brad Franklin, Durba Ghoshal, Jim Lor, Jennifer Massengil, Sofie Moens, Tyson Mooney, Dannyel Nelson, Karolien Peeters, Sashi Sathischyandra, Caroline Staut, Yoonhui Sung, Ann Tuttle, Annelies Van Hoecke, Annelies Van Raemdonck, Marie-Laure Verdegem, Steven Verhaeghe, Shane Walsh, Ann Wierckx, Qiang Zhao, Rozemarijn Dreesen   +29 more
openaire   +1 more source

Stability of expression of Neisseria gonorrhoeae lipooligosaccharides [PDF]

Infection and Immunity, 1986
We compared multiple lipooligosaccharide (LOS) extracts from individual strains of Neisseria gonorrhoeae. Each of the extracts was prepared from single mass cultures grown on solid media under similar conditions but separated by time. We found only subtle variations in the number, electrophoretic mobility, and concentration of components of the LOSs ...
H, Schneider, C A, Hammack, B A, Shuman, J M, Griffiss   +3 more
openaire   +2 more sources

Identification and Evaluation of Suitable Reference Genes for RT-qPCR Analysis in Hippodamia variegata (Coleoptera: Coccinellidae) Under Different Biotic and Abiotic Conditions

Frontiers in Physiology, 2021
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is an accurate and convenient technique for quantifying expression levels of the target genes.
Jiaoxin Xie, Tinghui Liu, Adel Khashaveh, Chaoqun Yi, Chaoqun Yi, Xiaoxu Liu, Xiaoxu Liu, Yongjun Zhang   +7 more
doaj   +1 more source

Selection and validation of optimal reference genes for RT-qPCR analyses in Aphidoletes aphidimyza Rondani (Diptera: Cecidomyiidae)

Frontiers in Physiology, 2023
Aphidoletes aphidimyza is a predator that is an important biological agent used to control agricultural and forestry aphids. Although many studies have investigated its biological and ecological characteristics, few molecular studies have been reported ...
Xiu-Xian Shen, Xiu-Xian Shen, Guo-Qiang Zhang, Yu-Xin Zhao, Xiao-Xiao Zhu, Xiao-Fei Yu, Mao-Fa Yang, Feng Zhang   +7 more
doaj   +1 more source

Coronaviruses as Vectors: Stability of Foreign Gene Expression [PDF]

Journal of Virology, 2005
ABSTRACT Coronaviruses are enveloped, positive-stranded RNA viruses considered to be promising vectors for vaccine development, as (i) genes can be deleted, resulting in attenuated viruses; (ii) their tropism can be modified by manipulation of their spike protein; and (iii) heterologous genes can be expressed by simply inserting ...
de Haan, C.A.M., Haijema, B.J., Boss, D., Heuts, F.W., Rottier, P.J.M.   +4 more
openaire   +4 more sources

Identification of Internal Reference Genes in Peripheral Blood Mononuclear Cells of Cattle Populations Adapted to Hot Arid Normoxia and Cold Arid Hypoxia Environments

Frontiers in Genetics, 2022
To estimate gene expression in a reliable manner, quantitative real-time polymerase chain reaction data require normalisation using a panel of stably expressed reference genes (RGs).
Preeti Verma, Ankita Sharma, Monika Sodhi, Manish Tiwari, Manish Tiwari, Prince Vivek, Ranjit S. Kataria, S. K. Nirajan, Vijay K. Bharti, Pawan Singh, Pawan Singh, S. S. Lathwal, S. S. Lathwal, Vishal Sharma, Nampher Masharing, Nampher Masharing, Manishi Mukesh   +16 more
doaj   +1 more source

Contractile acto-myosin network on nuclear envelope remnants positions human chromosomes for mitosis

eLife, 2019
To ensure proper segregation during mitosis, chromosomes must be efficiently captured by spindle microtubules and subsequently aligned on the mitotic spindle.
Alexander JR Booth, Zuojun Yue, John K Eykelenboom, Tom Stiff, GW Gant Luxton, Helfrid Hochegger, Tomoyuki U Tanaka   +6 more
doaj   +1 more source

Selection of Reference Genes for Transcription Studies Considering Co-Regulation and Average Transcriptional Stability: Case Study on Adventitious Root Induction in Olive (Olea europaea L.) Microshoots

Agronomy, 2022
Selection of reference genes (RGs) for normalization of PCR-gene expression data includes two crucial steps: determination of the between-sample transcriptionally more stable genes, and subsequent choosing of the most suitable genes as internal controls.
Carlos Noceda, Augusto Peixe, Birgit Arnholdt-Schmitt   +2 more
doaj   +1 more source