Results 11 to 20 of about 2,321,862 (234)

Genetic Stability, Inheritance Patterns and Expression Stability in Biotech Crops [PDF]

OBM Genetics, 2020
Demonstration of the stability of traits newly introduced into a plant genome via genetic engineering approaches comprise a significant portion of the safety assessment that these products undergo prior to receiving the requisite regulatory approvals enabling commercial authorization.
Laura Privalle, Patricia Back, Apurv Bhargava, Zach Bishop, Krystal Cisneros, Isabelle Coats, Ine Criel, Lien Dhondt, Travis Draughn, Barb Fowler, Brad Franklin, Durba Ghoshal, Jim Lor, Jennifer Massengil, Sofie Moens, Tyson Mooney, Dannyel Nelson, Karolien Peeters, Sashi Sathischyandra, Caroline Staut, Yoonhui Sung, Ann Tuttle, Annelies Van Hoecke, Annelies Van Raemdonck, Marie-Laure Verdegem, Steven Verhaeghe, Shane Walsh, Ann Wierckx, Qiang Zhao, Rozemarijn Dreesen   +29 more
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Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis in the Bean Bug, Riptortus pedestris (Hemiptera: Alydidae)

Insects, 2023
Quantitative real-time PCR (qRT-PCR) is widely accepted as a precise and convenient method for quantitatively analyzing the expression of functional genes. The data normalization strongly depends upon stable reference genes.
Liuyang Wang, Qingyu Liu, Pei Guo, Zhanlin Gao, Dan Chen, Tao Zhang, Jun Ning   +6 more
doaj   +1 more source

Stability of expression of Neisseria gonorrhoeae lipooligosaccharides [PDF]

Infection and Immunity, 1986
We compared multiple lipooligosaccharide (LOS) extracts from individual strains of Neisseria gonorrhoeae. Each of the extracts was prepared from single mass cultures grown on solid media under similar conditions but separated by time. We found only subtle variations in the number, electrophoretic mobility, and concentration of components of the LOSs ...
H, Schneider, C A, Hammack, B A, Shuman, J M, Griffiss   +3 more
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Transcriptional search to identify and assess reference genes for expression analysis in Solanumlycopersicum under stress and hormone treatment conditions

Journal of Integrative Agriculture, 2022
Tomato (Solanumlycopersicum) is a model plant for research on fruit development and stress response, in which gene expression analysis is frequently conducted.
Yao-ke DUAN, Rong HAN, Yan SU, Ai-ying WANG, Shuang LI, Hao SUN, Hai-jun GONG   +6 more
doaj   +1 more source

Hprt Serves as an Ideal Reference Gene for qRT-PCR Normalization in Rat DRG Neurons

Journal of Integrative Neuroscience, 2023
Objective: To identify suitable reference genes for gene expression studies in rat dorsal root ganglia (DRG) neurons. Methods: The raw cycle threshold (Ct) values of 12 selected reference genes were obtained via quantitative real-time reverse ...
Fenglan Liu, Yang Miao, Yaxian Wang, Qianqian Shan   +3 more
doaj   +1 more source

Identification and Evaluation of Suitable Reference Genes for RT-qPCR Analysis in Hippodamia variegata (Coleoptera: Coccinellidae) Under Different Biotic and Abiotic Conditions

Frontiers in Physiology, 2021
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is an accurate and convenient technique for quantifying expression levels of the target genes.
Jiaoxin Xie, Tinghui Liu, Adel Khashaveh, Chaoqun Yi, Chaoqun Yi, Xiaoxu Liu, Xiaoxu Liu, Yongjun Zhang   +7 more
doaj   +1 more source

Selection and validation of optimal reference genes for RT-qPCR analyses in Aphidoletes aphidimyza Rondani (Diptera: Cecidomyiidae)

Frontiers in Physiology, 2023
Aphidoletes aphidimyza is a predator that is an important biological agent used to control agricultural and forestry aphids. Although many studies have investigated its biological and ecological characteristics, few molecular studies have been reported ...
Xiu-Xian Shen, Xiu-Xian Shen, Guo-Qiang Zhang, Yu-Xin Zhao, Xiao-Xiao Zhu, Xiao-Fei Yu, Mao-Fa Yang, Feng Zhang   +7 more
doaj   +1 more source

Coronaviruses as Vectors: Stability of Foreign Gene Expression [PDF]

Journal of Virology, 2005
ABSTRACT Coronaviruses are enveloped, positive-stranded RNA viruses considered to be promising vectors for vaccine development, as (i) genes can be deleted, resulting in attenuated viruses; (ii) their tropism can be modified by manipulation of their spike protein; and (iii) heterologous genes can be expressed by simply inserting ...
de Haan, C.A.M., Haijema, B.J., Boss, D., Heuts, F.W., Rottier, P.J.M.   +4 more
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Identification of Internal Reference Genes in Peripheral Blood Mononuclear Cells of Cattle Populations Adapted to Hot Arid Normoxia and Cold Arid Hypoxia Environments

Frontiers in Genetics, 2022
To estimate gene expression in a reliable manner, quantitative real-time polymerase chain reaction data require normalisation using a panel of stably expressed reference genes (RGs).
Preeti Verma, Ankita Sharma, Monika Sodhi, Manish Tiwari, Manish Tiwari, Prince Vivek, Ranjit S. Kataria, S. K. Nirajan, Vijay K. Bharti, Pawan Singh, Pawan Singh, S. S. Lathwal, S. S. Lathwal, Vishal Sharma, Nampher Masharing, Nampher Masharing, Manishi Mukesh   +16 more
doaj   +1 more source

Contractile acto-myosin network on nuclear envelope remnants positions human chromosomes for mitosis

eLife, 2019
To ensure proper segregation during mitosis, chromosomes must be efficiently captured by spindle microtubules and subsequently aligned on the mitotic spindle.
Alexander JR Booth, Zuojun Yue, John K Eykelenboom, Tom Stiff, GW Gant Luxton, Helfrid Hochegger, Tomoyuki U Tanaka   +6 more
doaj   +1 more source