A number of transcriptome datasets for differential expression (DE) genes have been widely used for understanding organismal biology, but these datasets also contain untapped information that can be used to develop more precise analytical tools. With the
Jiaping Zhao +7 more
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A general approach for stabilizing nanobodies for intracellular expression
Conventional antibodies and their derived fragments are difficult to deploy against intracellular targets in live cells, due to their bulk and structural complexity.
John G Dingus +4 more
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Genomic stability and stability of expression in genetically modified plants [PDF]
Contains fulltext : 27748___.PDF (Publisher’s version ) (Open Access)
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Assessment of suitable reference genes for qRT-PCR analysis in Adelphocoris suturalis
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the most commonly-used tool for measurement of gene expression, but its accuracy and reliability depend on appropriate data normalization with the use of one or more stable ...
Jing LUO +8 more
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Stability of Facial Affective Expressions in Schizophrenia
Thirty-two videorecorded interviews were conducted by two interviewers with eight patients diagnosed with schizophrenia. Each patient was interviewed four times: three weekly interviews by the first interviewer and one additional interview by the second interviewer. 64 selected sequences where the patients were speaking about psychotic experiences were
H. Fatouros-Bergman +4 more
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Mouse HP1γ regulates TRF1 expression and telomere stability
Telomeric repeat-containing RNAs are long non-coding RNAs generated from the telomeres. TERRAs are essential for the establishment of heterochromatin marks at telomeres, which serve for the binding of members of the heterochromatin protein 1 (HP1) protein family of epigenetic modifiers involved with chromatin compaction and gene silencing.
Stylianakis, Emmanouil +7 more
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Identification of reference genes in blood before and after entering the plateau for SYBR green RT-qPCR studies [PDF]
Background Tibetans have lived at high altitudes for thousands of years, and they have unique physiological traits that enable them to tolerate this hypoxic environment. However, the genetic basis of these traits is still unknown.
Jun Xiao +5 more
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Screening of reference genes for expression analysis in the study of soldier caste differentiation of Formosan subterranean termite Coptotermes formosanus Shiraki [PDF]
The Formosan subterranean termite, Coptotermes formosanus Shiraki, is a serious pest worldwide. Juvenile hormone analog (and its analogs such as methoprene) can induce the transformation of the worker caste into soldier caste in C. formosanus.
He Du +3 more
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Impact of RNA quality on reference gene expression stability
Gene expression quantification methods are important tools in the understanding of the molecular events underlying human diseases and in the identification of diagnostic and therapeutic targets. Generally, the messenger RNA (mRNA) used for these analyses is derived from human biopsies obtained after surgery.
Claudina Angela Pérez-Novo +5 more
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Thrombospondin expression in myofibers stabilizes muscle membranes
Skeletal muscle is highly sensitive to mutations in genes that participate in membrane stability and cellular attachment, which often leads to muscular dystrophy. Here we show that Thrombospondin-4 (Thbs4) regulates skeletal muscle integrity and its susceptibility to muscular dystrophy through organization of membrane attachment complexes.
Davy Vanhoutte +13 more
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