Real-time in vivo imaging of extracellular ATP in the brain with a hybrid-type fluorescent sensor
eLife, 2020 Adenosine 5’ triphosphate (ATP) is a ubiquitous extracellular signaling messenger. Here, we describe a method for in-vivo imaging of extracellular ATP with high spatiotemporal resolution.
Nami Kitajima +11 more
doaj +1 more source
Coexpression of rat P2X2 and P2X6 subunits in Xenopus oocytes. [PDF]
, 2000 Transcripts for P2X(2) and P2X(6) subunits are present in rat CNS and frequently colocalize in the same brainstem nuclei. When rat P2X(2) (rP2X(2)) and rat P2X(6) (rP2X(6)) receptors were expressed individually in Xenopus oocytes and studied under ...
Burnstock, G +5 more
core +1 more source
Conserved Extracellular Cysteines Differentially Regulate the Potentiation Produced by Zn2+ in Rat P2X4 Receptors [PDF]
, 2013 One feature of the amino acid sequence of P2X receptors identified from mammalian species, Xenopus laevis and zebrafish is the conservation of ten cysteines in the extracellular loop.
Chen, Lin +7 more
core +2 more sources
Activation of retinal glial (Müller) cells by extracellular ATP induces pronounced increases in extracellular H+ flux. [PDF]
PLoS ONE, 2018 Small alterations in extracellular acidity are potentially important modulators of neuronal signaling within the vertebrate retina. Here we report a novel extracellular acidification mechanism mediated by glial cells in the retina. Using self-referencing
Boriana K Tchernookova +10 more
doaj +1 more source
Ontogeny of purinergic receptor-regulated Ca2+ signaling in mouse cortical collecting duct epithelium [PDF]
, 2002 Changes in ATP-induced increase in {[}Ca2+], during collecting duct ontogeny were studied in primary monolayer cultures of mouse ureteric bud (UB) and cortical collecting duct (CCD) cells by Fura-PE3 fluorescence ratio imaging.
Borscheid, R. +4 more
core +1 more source
Sensors, 2021 Adenosine 5′-triphosphate (ATP) plays a crucial role as an extracellular signaling molecule in the central nervous system and is closely related to various nerve diseases.
Hideo Doi +5 more
doaj +1 more source
dATP/ATP, a multifunctional nucleotide, stimulates bacterial cell lysis, extracellular DNA release and biofilm development. [PDF]
PLoS ONE, 2010 Signaling by extracellular adenosine 5'-triphosphase (eATP) is very common for cell-to-cell communication in many basic patho-physiological development processes.
Chuanwu Xi, Jianfeng Wu
doaj +1 more source
ENaC, renal sodium excretion and extracellular ATP [PDF]
Purinergic Signalling, 2009 Sodium balance determines the extracellular fluid volume and sets arterial blood pressure (BP). Chronically raised BP (hypertension) represents a major health risk in Western societies. The relationship between BP and renal sodium excretion (the pressure/natriuresis relationship) represents the key element in defining the BP homeostatic set point.
Wildman, Scott S.P. +2 more
openaire +3 more sources
Activation of the P2Y2 receptor regulates bone cell function by enhancing ATP release [PDF]
, 2017 Bone cells constitutively release ATP into the extracellular environment where it acts locally via P2 receptors to regulate bone cell function. Whilst P2Y2 receptor stimulation regulates bone mineralisation, the functional effects of this receptor in ...
Arnett, T R +5 more
core +2 more sources
Extracellular ATP released by osteoblasts is a key local inhibitor of bone mineralisation [PDF]
, 2013 Previous studies have shown that exogenous ATP (>1µM) prevents bone formation in vitro by blocking mineralisation of the collagenous matrix. This effect is thought to be mediated via both P2 receptor-dependent pathways and a receptor-independent ...
A Brandao-Burch +49 more
core +3 more sources