Results 41 to 50 of about 604,290 (256)

Homeostasis of Extracellular ATP in Human Erythrocytes [PDF]

Journal of Biological Chemistry, 2011
استكشفنا العمليات داخل وخارج الخلايا التي تحكم حركية ATP خارج الخلايا (ATPe) في كريات الدم الحمراء البشرية المحفزة بالعوامل التي تزيد من المخيم. باستخدام تفاعل لوسيفيرين- لوسيفيراز في قياس الإضاءة خارج الخط، وجدنا تنشيطًا مباشرًا لأنزيم الأدينيل سايكلز بواسطة فورسكولين وتنشيطًا غير مباشر من خلال التحفيز الأدرينالي بيتا مع [ATP]e المعزز بالإيزوبروتينول ...
Nicolás Montalbetti, María Florencia Leal Denis, Omar P. Pignataro, Eiry Kobatake, Eduardo R. Lazarowski, Pablo J. Schwarzbaum   +5 more
openaire   +2 more sources

Release of ATP from marginal cells in the cochlea of neonatal rats can be induced by changes in extracellular and intracellular ion concentrations. [PDF]

PLoS ONE, 2012
BackgroundAdenosine triphosphate (ATP) plays an important role in the cochlea. However, the source of ATP and the mechanism by which it is released remain unclear.
Yating Peng, Jie Chen, Shan He, Jun Yang, Hao Wu   +4 more
doaj   +1 more source

Activity-dependent release of Adenosine: a critical re-evaluation of mechanism [PDF]

, 2008
Adenosine is perhaps the most important and universal modulator in the brain. The current consensus is that it is primarily produced in the extracellular space from the breakdown of previously released ATP.
Dale, Nicholas, Wall, Mark J.
core   +1 more source

Simultaneous Fluorescent Recordings of Extracellular ATP and Intracellular Calcium in Mammalian Cells

Bio-Protocol, 2019
Extracellular ATP is a potent signaling molecule that stimulates intracellular calcium responses through purinergic (P2) receptors in mammalian cells. While extracellular ATP and intracellular calcium can be measured separately, simultaneous monitoring ...
Nicholas Mikolajewicz, Svetlana Komarova
doaj   +1 more source

An Improved Ratiometric FRET Biosensor with Higher Affinity for Extracellular ATP

Sensors
Adenosine triphosphate (ATP) is readily released into the extracellular space as an autocrine and paracrine purinergic signaling molecule. We originally reported a genetically encoded, fluorescent protein-based Förster Resonance Energy Transfer (FRET ...
Autumn Cholger, Jason M. Conley, Elaine Colomb, Olivia de Cuba, Jacob Kress, Mathew Tantama   +5 more
doaj   +1 more source

Hepatocyte heterogeneity in response to extracellular ATP [PDF]

European Journal of Biochemistry, 1987
The metabolic and hemodynamic effects of extracellular ATP in perfused rat liver were compared during physiologically antegrade (portal to hepatic vein) and retrograde (hepatic to portal vein) perfusion. ATP in concentrations up to 100 μM was completely hydrolyzed during a single liver passage regardless of the perfusion direction.
D, Häussinger, T, Stehle, W, Gerok, T A, Tran-Thi, K, Decker   +4 more
openaire   +2 more sources

Do K_ATP channels open as a prominent and early feature during ischaemia in the Langendorff-perfused rat heart? [PDF]

, 2000
The objective was to investigate whether myocardial adenosine triphosphate-sensitive K⁺ (K_ATP) channels open during the first 10 min of regional ischaemia in Langendorff-perfused rat hearts.
Northover, B.J., MacKenzie, I., Workman, A.J.   +2 more
core   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

P2X receptors: epithelial ion channels and regulators of salt and water transport. [PDF]

, 2008
When the results from electrophysiological studies of renal epithelial cells are combined with data from in vivo tubule microperfusion experiments and immunohistochemical surveys of the nephron, the accumulated evidence suggests that ATP-gated ion ...
King, Brian F., Wildman, Scott S.P.
core   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source