Results 31 to 40 of about 215,428 (267)

Secreted exosomes induce filopodia formation

eLife
Filopodia are dynamic adhesive cytoskeletal structures that are critical for directional sensing, polarization, cell-cell adhesion, and migration of diverse cell types. Filopodia are also critical for neuronal synapse formation.
Caitlin McAtee, Mikin R Patel, Daisuke Hoshino, Bong Hwan Sung, Ariana K von Lersner, Mingjian Shi, Nan Hyung Hong, Anna Young, Evan Krystofiak, Andries Zijlstra, Alissa M Weaver   +10 more
doaj   +1 more source

Engineered IL-15/IL-15Rα-expressing cellular vesicles promote T cell anti-tumor immunity

Extracellular Vesicle, 2023
Interleukin 15 (IL-15) is a pivotal immune cytokine that responses to cancer stress signals and maintains tissue-resident memory T (TRM) cells. It is therefore considered as a potent therapeutic immunomodulator agonist cancer. However, in addition to the
Wenli Fang, Liyan Li, Zhongda Lin, Yuli Zhang, Zhangyan Jing, Yuan Li, Zhirang Zhang, Linlin Hou, Xin Liang, Xingding Zhang, Xudong Zhang   +10 more
doaj   +1 more source

A ‘torn bag mechanism’ of small extracellular vesicle release via limiting membrane rupture of en bloc released amphisomes (amphiectosomes)

eLife
Recent studies showed an unexpected complexity of extracellular vesicle (EV) biogenesis pathways. We previously found evidence that human colorectal cancer cells in vivo release large multivesicular body-like structures en bloc.
Tamás Visnovitz, Dorina Lenzinger, Anna Koncz, Péter M Vizi, Tünde Bárkai, Krisztina V Vukman, Alicia Galinsoga, Krisztina Németh, Kelsey Fletcher, Zsolt I Komlósi, Csaba Cserép, Ádám Dénes, Péter Lőrincz, Gábor Valcz, Edit I Buzas   +14 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Organizing the interface—Plasma membrane architecture and receptor dynamics in virus‐cell interactions

FEBS Letters, EarlyView.
Plasma membranes contain dynamic nanoscale domains that organize lipids and receptors. Because viruses operate at similar scales, this architecture shapes early infection steps, including attachment, receptor engagement, and entry. Using influenza A virus and HIV‐1 as examples, we highlight how receptor nanoclusters, multivalent glycan interactions ...
Jan Schlegel, Christian Sieben
wiley   +1 more source

5‐Fluorouracil treatment represses pseudouridine‐containing miRNA export into extracellular vesicles

Journal of Extracellular Biology
5‐Fluorouracil (5‐FU) has been used for chemotherapy for colorectal and other cancers for over 50 years. The prevailing view of its mechanism of action is inhibition of thymidine synthase leading to defects in DNA replication and repair. However, 5‐FU is
Shimian Qu, Hannah M. Nelson, Xiao Liu, Yu Wang, Elizabeth M. Semler, Danielle L. Michell, Clark Massick, Jeffrey L. Franklin, John Karijolich, Alissa M. Weaver, Robert J. Coffey, Qi Liu, Kasey C. Vickers, James G. Patton   +13 more
doaj   +1 more source

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

Cholesteryl esters and high protein-to-lipid ratios distinguish non-vesicular extracellular particles from extracellular vesicles

Cell Communication and Signaling
Background Mechanisms governing non-vesicular extracellular particle (NVEP) biogenesis and compositional diversity remain incompletely characterized. Method This study integrates multiparametric profiling to define NVEPs.
Sayam Ghosal, Rita Leporati, Árpád Varga, Petra Susanszki, Nóra Fekete, Tamás László, Tünde Bárkai, Fülöp Károly Grébecz, Delaram Khamari, Tárek Zoltán Magyar, Marcus Hoering, Krisztina V Vukman, Bernadett R Bodnár, Brachyahu M Kestecher, Mohamed A Fattah, Csaba Bödör, József Maléth, Gerhard Liebisch, Evelyn Orsó, Edit I Buzas, Xabier Osteikoetxea   +20 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Exofection by exosomes: A transient functional cargo transfer

Extracellular Vesicle
Exosomes have emerged as key mediators of inter-cellular communication, transporting a diverse array of cargos that can reflect the current biological state of the cell.
Ramkumar Menon, Madhuri Tatiparthy, Jessica Selim, Isidore Mushimiyimana, Brinley Harrington, Awanit Kumar, Lauren Richardson, Emmanuel Amabebe, Ananth Kumar Kammala   +8 more
doaj   +1 more source