Results 211 to 220 of about 4,584 (262)
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Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1989
Bovine liver catalase (hydrogen-peroxide:hydrogen peroxide oxidoreductase, EC 1.11.1.6) was derivatized by 9"(10")-[4'-(2-(4,6-dichloro-1,3,5-triazinyl) oxy)butoxy] stearic acid and the fatty acyl-coated enzyme was separated from native catalase and excess reagent by hydroxyapatite chromatography. The derivatization of catalase resulted in coupling the
G, Rosen, J, Bar-Tana
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Bovine liver catalase (hydrogen-peroxide:hydrogen peroxide oxidoreductase, EC 1.11.1.6) was derivatized by 9"(10")-[4'-(2-(4,6-dichloro-1,3,5-triazinyl) oxy)butoxy] stearic acid and the fatty acyl-coated enzyme was separated from native catalase and excess reagent by hydroxyapatite chromatography. The derivatization of catalase resulted in coupling the
G, Rosen, J, Bar-Tana
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Fatty acylation of Wnt proteins
Nature Chemical Biology, 2016Wnt proteins are critical regulators of signaling networks during embryonic development and in adult tissue homeostasis. The generation of active Wnt proteins requires their regulated secretion into the extracellular space. Once secreted, Wnts signal through the cell surface via receptor binding on Wnt-receiving cells, a mechanism that is prevalent in ...
Aaron H, Nile, Rami N, Hannoush
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Archives of Biochemistry and Biophysics, 1968
Abstract The addition of fatty acid, ATP, Mg 2+ and CoA to a rat liver mitochondrial suspension promoted swelling of the mitochondria which was reversed by the addition of carnitine. The carnitine-mediated contraction was blocked by respiratory inhibitors, and was accompanied by an increased formation of mitochondrial fatty acyl carnitine.
J, Kuttis, M, Nakatani, W C, McMurray
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Abstract The addition of fatty acid, ATP, Mg 2+ and CoA to a rat liver mitochondrial suspension promoted swelling of the mitochondria which was reversed by the addition of carnitine. The carnitine-mediated contraction was blocked by respiratory inhibitors, and was accompanied by an increased formation of mitochondrial fatty acyl carnitine.
J, Kuttis, M, Nakatani, W C, McMurray
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Biochemical and Biophysical Research Communications, 1971
Abstract In the synthesis of phosphatidic acid catalyzed by a particulate fraction from Clostridium butyricum in the presence of fatty acyl derivatives of an acyl carrier protein (ACP) and sn -glycero-3-phosphate, the first acylation of glycero-3-P is more rapid with an unsaturated acyl-ACP than with a saturated acyl-ACP.
H, Goldfine, G P, Ailhaud
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Abstract In the synthesis of phosphatidic acid catalyzed by a particulate fraction from Clostridium butyricum in the presence of fatty acyl derivatives of an acyl carrier protein (ACP) and sn -glycero-3-phosphate, the first acylation of glycero-3-P is more rapid with an unsaturated acyl-ACP than with a saturated acyl-ACP.
H, Goldfine, G P, Ailhaud
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Immunogold labelling of fatty acyl chains
Journal of Immunological Methods, 1995For the first time, antibodies against a hydrophobic hapten have been used for immunogold labelling of a lipid antigen (BSA-C18:1 conjugate) coated on polystyrene. The labelling was visualised either directly in transmission electron microscopy or in light microscopy after silver enhancement.
Compère, Philippe +3 more
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The Critical Micellar Concentrations of Fatty Acyl Coenzyme A and Fatty Acyl Carnitines
Journal of Colloid and Interface Science, 1993Abstract The critical micellar concentrations of various acyl CoA and acyl carnitines have been measured using a method based on the optical absorption, in the visible range, of the dye merocyanine 540. Values for palmitoyl-CoA and palmitoyl carnitine in 50 m M potassium phosphate, pH 7.4, at 22°C are, respectively, 35 μ M and 15 μ M .
M.Asun Requero +2 more
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Fatty Acyl-Coenzyme A Dehydrogenases
Biochemical Society Transactions, 1978The general enzymology of @-oxidation has suffered extraordinary neglect since the broad outlines of the pathway were established in the 1950’s. For the acyl-CoA dehydrogenases the contributions from the Institute for Enzyme Research at the University of Wisconsin still represent the major part of our knowledge.
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Archives of Biochemistry and Biophysics, 1988
The critical micelle concentrations (CMCs) of palmitoyl-CoA/stearoyl-CoA and palmitoyl-CoA/oleoyl-CoA mixtures in 0.050 M KPi, pH 7.4, a buffer used in enzymatic studies, were determined by fluorescence. Mixed micelle solution theory, analogous to the thermodynamic treatment of vapor pressure, was applied to calculate monomer and micelle compositions ...
P P, Constantinides, J M, Steim
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The critical micelle concentrations (CMCs) of palmitoyl-CoA/stearoyl-CoA and palmitoyl-CoA/oleoyl-CoA mixtures in 0.050 M KPi, pH 7.4, a buffer used in enzymatic studies, were determined by fluorescence. Mixed micelle solution theory, analogous to the thermodynamic treatment of vapor pressure, was applied to calculate monomer and micelle compositions ...
P P, Constantinides, J M, Steim
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Structure-function correlation of fatty acyl-CoA dehydrogenase and fatty acyl-CoA oxidase
Biochemistry, 1985We have employed a new pseudosubstrate, beta-(2-furyl)propionyl coenzyme A (FPCoA), to study the functional properties of two enzymes, fatty acyl-CoA dehydrogenase from porcine liver and fatty acyl-CoA oxidase from Candida tropicalis, involved in the oxidation of fatty acids.
C, Rojas +4 more
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Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1970
1. 1. A particulate fraction of Escherichia coli catalyzes the transfer of palmitate from palmityl-CoA and palmityl-acyl carrier protein to sn-glycero-3-phosphate. When a series of long-chain acyl thiolesters including myristyl-, palmityl-, palmitoleyl- and cis-vaccenyl-CoA and acyl carrier protein were tested in the acylation of sn-glycero-3 ...
H. Van Den Bosch, P.Roy Vagelos
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1. 1. A particulate fraction of Escherichia coli catalyzes the transfer of palmitate from palmityl-CoA and palmityl-acyl carrier protein to sn-glycero-3-phosphate. When a series of long-chain acyl thiolesters including myristyl-, palmityl-, palmitoleyl- and cis-vaccenyl-CoA and acyl carrier protein were tested in the acylation of sn-glycero-3 ...
H. Van Den Bosch, P.Roy Vagelos
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