Results 71 to 80 of about 20,810 (248)

In Vivo CRISPR Screening Identifies the Glutamate Receptor GRIA2 as Promoting Peritoneal Metastasis of Gastric Cancer via Calcium‐Dependent β‐Catenin Activation

Advanced Science, EarlyView.
We identified GRIA2 as a critical driver of gastric cancer peritoneal metastasis through in vivo CRISPR screening. Mechanistically, GRIA2‐mediated calcium influx inhibits GSK3β and activates Wnt/β‐catenin signaling, driven by glutamate from cancer‐associated fibroblasts.
Jie Sun, Xu Yang, Wenchao Jiang, Chengbo Ji, Yingying Wu, Haoyu Sun, Xinyou Liu, Masami Yamamoto, Tetsuya Tsukamoto, Sachiyo Nomura, Junjie Zhao, Yuanyuan Ruan, Haojie Li, Xuefei Wang   +13 more
wiley   +1 more source

In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection

Parasites & Vectors, 2014
Background The development of techniques that allow the imaging of animals infected with parasites expressing luciferase opens up new possibilities for following the fate of parasites in infected mammals. Methods D-luciferin potassium salt stock solution
Cristina Henriques, Andréa Henriques-Pons, Marcelo Meuser-Batista, Aline Salgado Ribeiro, Wanderley de Souza   +4 more
doaj   +1 more source

Maize Anther Development Involves Translated Open Reading Frames From 3′ Untranslated Regions

Advanced Science, EarlyView.
This study examined the translation activity across ten stages of maize anther development, integrating transcriptome and small‐peptide data. It linked mutations in translated 3' ORFs to anther sterility, exemplified by Zm00001eb417050_3' ORF (known as APV1). In addition, the data indicated that maize anthers could assimilate CO2 through the stomata in
Chunyu Wang, Yuechao Wu, Shunxi Wang, Han Liu, Siqi Jiang, Yingjia Han, Liuji Wu, Tao Zhang, Mei Zhang   +8 more
wiley   +1 more source

Use of the enzyme hexokinase for the reduction of inherent light levels [PDF]

, 1969
Use of enzyme hexokinase and glucose to reduce inherent light levels of ATP in luciferase ...
Chappelle, E. W.
core   +1 more source

A PCA3 gene-based transcriptional amplification system targeting primary prostate cancer. [PDF]

, 2015
Targeting specifically primary prostate cancer (PCa) cells for immune therapy, gene therapy or molecular imaging is of high importance. The PCA3 long non-coding RNA is a unique PCa biomarker and oncogene that has been widely studied.
Fradet, Yves, Jain, Pallavi, Neveu, Bertrand, Pouliot, Frédéric, Têtu, Bernard, Wu, Lily   +5 more
core   +2 more sources

OsGSK2‐OsTCP19 Module Integrates Nitrogen and Brassinosteroid Signaling to Regulate Nitrogen Utilization and Root Growth in Rice

Advanced Science, EarlyView.
OsGSK2‐OsTCP19 module regulating nitrate response and lateral root (LR) development. Low‐nitrate condition results in reduced BR response, accumulation of OsGSK2 and phosphorylated OsTCP19, which suppresses the expression of nitrate‐responsive genes and LR‐development genes and impairs rice growth.
Yongqiang Liu, Weiwei Li, Xiaohui Ma, Xiaohan Wang, Yarong Shi, Xiahe Huang, Xinran Li, Yingchun Wang, Hongning Tong, Bin Hu, Wenbin Zhou, Chengcai Chu   +11 more
wiley   +1 more source

Analytical Applications of Bioluminescence and Chemiluminescence [PDF]

Bioluminescence and chemiluminescence studies were used to measure the amount of adenosine triphosphate and therefore the amount of energy available. Firefly luciferase - luciferin enzyme system was emphasized.
Chappelle, E. W., Picciolo, G. L.
core   +1 more source

Autophagy Plays a Suppressive Role in Bladder Tumor Formation in an Orthotopic Mouse Model and Bladder Cancer Patient Specimens

The Kaohsiung Journal of Medical Sciences, EarlyView.
ABSTRACT Autophagy plays either a suppressing or promoting role during tumor development. Clarifying the role of autophagy in bladder tumorigenesis both in vitro and in vivo is crucial for developing novel therapeutic strategies through manipulating autophagy activity.
Wan‐Ting Kuo, Chin‐Chen Pan, Yi‐Wen Liu, Nan‐Haw Chow, Hong‐Lin Cheng, Shan‐Ying Wu, Sheng‐Hui Lan, Chih‐Peng Chang, Hsiao‐Sheng Liu   +8 more
wiley   +1 more source

A Dual-Color Bioluminescence Reporter Mouse for Simultaneous in vivo Imaging of T Cell Localization and Function

Frontiers in Immunology, 2019
Non-invasive imaging technologies to visualize the location and functionality of T cells are of great value in immunology. Here, we describe the design and generation of a transgenic mouse in which all T cells constitutively express green-emitting click ...
Jan Willem Kleinovink, Laura Mezzanotte, Laura Mezzanotte, Giorgia Zambito, Giorgia Zambito, Giorgia Zambito, Marieke F. Fransen, Luis J. Cruz, J. Sjef Verbeek, Alan Chan, Ferry Ossendorp, Clemens Löwik, Clemens Löwik, Clemens Löwik   +13 more
doaj   +1 more source

Interleukin‐10 Derived Apoptotic Vesicles Enhance Scarless Skin Healing by Modulating Fibroblast Metabolism and Fibrosis Pathways

Cell Proliferation, EarlyView.
IL10_ApoEVs, generated during IL‐10‐induced fibroblast apoptosis, reprogram fibroblast metabolism and inhibit fibrosis to promote scarless skin healing. By enhancing mitochondrial OXPHOS, suppressing glycolysis, and downregulating Hedgehog signaling, IL10_ApoEVs shift collagen deposition toward regenerative type III collagen, offering a novel strategy ...
Yang Zou, Jinglun Zhang, Wenxuan Mao, Shuting Jiang, Cheng Xu, Jiayi Meng, Heng Dong, Yongbin Mou   +7 more
wiley   +1 more source