Results 51 to 60 of about 14,327 (227)

Screening of the Pathogen Box for inhibitors with dual efficacy against Giardia lamblia and Cryptosporidium parvum. [PDF]

PLoS Neglected Tropical Diseases, 2018
There is need for a more efficient cell-based assay amenable to high-throughput drug screening against Giardia lamblia. Here, we report the development of a screening method utilizing G.
Kelly M Hennessey, Ilse C Rogiers, Han-Wei Shih, Matthew A Hulverson, Ryan Choi, Molly C McCloskey, Grant R Whitman, Lynn K Barrett, Ethan A Merritt, Alexander R Paredez, Kayode K Ojo   +10 more
doaj   +1 more source

Master Regulator SMC1A, Stabilized by N6‐Methyladenosine Reader IGF2BP1, Promotes HCC Progression Through Facilitating Enhancer–Promoter Interaction of Nestin

Advanced Science, EarlyView.
IGF2BP1‐mediated m6A stabilization sustains SMC1A expression, enabling cohesin‐associated chromatin regulation of Nestin in hepatocellular carcinoma. This work reveals an epitranscriptomic‐chromatin‐cytoskeletal regulatory axis linked to malignant phenotypes and identifies SMC1A as a biologically relevant vulnerability in HCC.
Zhenxiang Peng, Diguang Wen, Lu Zeng, Lin Lv, Shengtao Liao, Wenguang Zhang, Zhechuan Mei, Chuanfei Li   +7 more
wiley   +1 more source

Aminoluciferins extend firefly luciferase bioluminescence into the near-infrared and can be preferred substrates over D-luciferin

, 2017
Firefly luciferase adenylates and oxidizes d-luciferin to chemically generate visible light and is widely used for biological assays and imaging. Here we show that both luciferase and luciferin can be reengineered to extend the scope of this light ...
Reddy, Gadarla Randheer, Mofford, David M., Miller, Stephen C.   +2 more
core   +1 more source

Optimisation of bioluminescent reporters for use with mycobacteria [PDF]

, 2010
Mycobacterium tuberculosis, the causative agent of tuberculosis, still represents a major public health threat in many countries. Bioluminescence, the production of light by luciferase-catalyzed reactions, is a versatile reporter technology with multiple
Jorge Ripoll, Fletcher, T, Theresa H Ward, Ripoll, Jorge, Ward, Theresa H, Ripoll, J, Parish, T, Paul T Elkington, Andrea Zelmer, Robertson, Brian D, Tanya Parish, Fletcher, Taryn, Andreu, Nuria, Robertson, BD, Bancroft, Gregory J., Wiles, Siouxsie, Siouxsie Wiles, Ward, Theresa H., Gregory J Bancroft, Ward, TH, Brian D Robertson, Nuria Andreu, Ulrich Schaible, Bancroft, GJ, Andreu, N, Parish, Tanya, Robertson, Brian D., Schaible, U, Wiles, S, Schaible, Ulrich, Elkington, PT, Bancroft, Gregory J, Zelmer, Andrea, Elkington, Paul T, Taryn Fletcher, Elkington, Paul T., Zelmer, A   +36 more
core   +1 more source

PIEZO1 is Required for Acute Myeloid Leukemia Progression and Leukemia Stem Cell Maintenance via HIF1A‐SLC7A11 Axis‐Mediated Ferroptosis Defense

Advanced Science, EarlyView.
This study identifies the mechanosensor PIEZO1 as a key factor in promoting acute myeloid leukemia progression and uncovers a ferroptosis defense system mediated by PIEZO1‐HIF1A‐SLC7A11 axis that is essential for leukemia stem cell maintenance. This ferroptotic defense system may represent a unique vulnerability for leukemia stem cells, providing a ...
Tiantian Zhang, Ziyan Cao, Kexin Gao, Shuxin Yao, Yanbing Zheng, Manman Cui, Rong Yin, Guoqiang Han, Jin Hu, Chengyi Wang, Haojian Zhang   +10 more
wiley   +1 more source

Codon-Optimized Luciferase Variants for Mammalian Gene Expression in Culture and in Vivo

Molecular Imaging, 2012
Luciferases have proven to be useful tools in advancing our understanding of biologic processes. Having a multitude of bioluminescent reporters with different properties is highly desirable.
Casey A. Maguire, Johannes C. van der Mijn, Marja H. Degeling, Danielle Morse, Bakhos A. Tannous   +4 more
doaj   +1 more source

Allosteric Inhibition of Polycomb Repressive Complex 2 by an EZH2‐Selective Small Molecule Inhibitor

Advanced Science, EarlyView.
The study characterizes C36, a highly selective EZH2/PRC2 inhibitor that acts via a novel allosteric mechanism. Unlike previous inhibitors, C36 inhibits EZH2/PRC2 by disrupting the allosteric communication between EZH2 and EED in a SAM‐noncompetitive manner.
Ting Cao, Dongdong Liu, Haishan Gao, Chenyang Qin, Wen Zhang, Ziyun Lu, Dongxia Tan, Yuxiu Qu, Yusong Liu, Zhiran Zou, Hongtao Yu, Wei Qi   +11 more
wiley   +1 more source

Novel Heterocyclic Analogues of Firefly Luciferin

, 2016
Five novel firefly luciferin analogues in which the benzothiazole ring system of the natural substrate was replaced with benzimidazole, benzofuran, benzothiophene, benzoxazole, and indole were synthesized.
Justin C. Rosenberg (2014330), Bruce R. Branchini (1332534), Tara L. Southworth (1578652), Carolyn C. Woodroofe (1363851), Curran E. Behney (1736278), Dieter H. Klaubert (1290432), Yumi Kovic (2014327), Poncho L. Meisenheimer (2014333)   +7 more
core   +1 more source

A Conserved DT2‐bZIP66‐NF‐YC4 Regulatory Module Confers Drought Tolerance in Rice and Arabidopsis

Advanced Science, EarlyView.
This study identifies a conserved tripartiteDT2–bZIP66–NF‐YC4 transcriptional module that enhances rice drought tolerance by activating stress‐responsive genes. This regulatory complex is functionally conserved across cereals and Arabidopsis, providing promising targets for engineering drought‐resilient crops. ABSTRACT Drought stress severely restricts
Jun Shen, Yuchen Li, Liang Zhang, Xiaosong Ma, Chenyin Peng, Chunting Yuan, Yu Wu, Kaiyu Zhao, Sheng Zhao, Jiazhuo Guo, Run Li, Ziqian Yan, Shule Ren, Yige Han, Xin Lin, Xinyue Li, Ming Li, Pingfang Yang, Shiyong Song   +18 more
wiley   +1 more source

Inhibition of KDEL Receptors Remodels the Tumor Microenvironment for T Cell Independent Tumor Regression

Advanced Science, EarlyView.
Inhibition of KDELR2 in a small fraction of tumor cells generates sustainable immunogenic cell death conditions within the tumor microenvironment. These conditions promote the regression of tumors in a T cell independent manner. During regression, macrophages prime T cells that subsequently provide systemic protection against recurrence. The potency of
Shakti P Pattanayak, Hong Wang, Belinda Willard, Timothy A. Chan, William C Merrick, Zheng‐Rong Lu, Boaz Tirosh   +6 more
wiley   +1 more source