Scientific Reports, 2020 Fireflies are among the most charismatic insects for their spectacular bioluminescence, but the origin and evolution of bioluminescence remain elusive.
Ru Zhang +23 more
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Using bioluminescence to image gene expression and spontaneous behavior in freely moving mice.
PLoS ONE, 2023 Bioluminescence imaging (BLI) of gene expression in live animals is a powerful method for monitoring development, tumor growth, infections, healing, and other progressive, long-term biological processes. BLI remains an effective approach for reducing the
Astha Malik +2 more
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Molecular Therapy: Oncolytics, 2021 Bioluminescence reporter gene imaging is a robust, high-throughput imaging modality that is useful for tracking cells and monitoring biological processes, both in cell culture and in small animals.
Maxim A. Moroz +10 more
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Redox-triggerable firefly luciferin-bioinspired hydrogels as injectable and cell-encapsulating matrices [PDF]
, 2022 Stimuli-responsive hydrogels are smart materials that respond to variations caused by external stimuli and that are currently exploited for biomedical applications such as biosensing, drug delivery and tissue engineering.
Jin, Minye +5 more
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Optogenetic inhibition of neurons by internal light production
Frontiers in Behavioral Neuroscience, 2014 Optogenetics is an extremely powerful tool for selective neuronal activation/inhibition and dissection of neural circuits. However, a limitation of in vivo optogenetics is that an animal must be tethered to an optical fiber for delivery of light.
Benjamin eLand +4 more
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A photocytes-associated fatty acid-binding protein from the light organ of adult Taiwanese firefly, Luciola cerata. [PDF]
PLoS ONE, 2011 BACKGROUND: Intracellular fatty acid-binding proteins (FABPs) are considered to be an important energy source supplier in lipid metabolism; however, they have never been reported in any bioluminescent tissue before.
King-Siang Goh, Chia-Wei Li
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Influence of Bioluminescence Imaging Dynamics by D-Luciferin Uptake and Efflux Mechanisms
Molecular Imaging, 2012 Bioluminescence imaging (BLI) detects light generated by luciferase-mediated oxidation of substrate and is used widely for evaluating transgene expression in cell-based assays and in vivo in relevant preclinical models.
Yimao Zhang +3 more
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Decrease of catalytic efficiency of Photinus pyralis firefly luciferase in the presence of graphene quantum dots [PDF]
Nanomedicine Journal, 2020 Objective(s): Firefly luciferase is a monooxygenase enzyme that emits flash of light during the enzymatic reaction. Luciferase has been used in many bioanalytical fields from ATP detection methods to in vivo imaging.
Elaheh Samadi +3 more
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Fluorescent Properties of Firefly Luciferases and Their Complexes with Luciferin
Bioscience Reports, 2000 Fluorescence of luciferases from Luciola mingrelica (single tryptophanresidue, Trp-419) and Photinus pyralis (two tryptophan residues, Trp-417,Trp-426) was studied. Analysis of quenching of tryptophan fluorescenceshowed that the tryptophan residue conserved in all luciferases is notaccessible for charged quenchers, which is explained by the presence ...
E I, Dementieva +4 more
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Regulatory effects of ATP and luciferin on firefly luciferase activity [PDF]
Biochemical Journal, 1995 ATP and luciferin are not only substrates of firefly luciferase, but can, in addition, modulate its activity. High concentrations of luciferin induce a conformational change of the enzyme that temporarily reduces the catalytic rate. Re-activation takes approx.
N, Lembert, L A, Idahl
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