Results 21 to 30 of about 21,179 (270)

A Novel Brighter Bioluminescent Fusion Protein Based on ZZ Domain and Amydetes vivianii Firefly Luciferase for Immunoassays

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Immunoassays are widely used for detection of antibodies against specific antigens in diagnosis, as well as in electrophoretic techniques such as Western Blotting.
Vadim R. Viviani   +4 more
doaj   +1 more source

Improving the Stability of Protein–Protein Interaction Assay FlimPIA Using a Thermostabilized Firefly Luciferase

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
The protein–protein interaction assay is a key technology in various fields, being applicable in drug screening as well as in diagnosis and inspection, wherein the stability of assays is important.
Yuki Ohmuro-Matsuyama   +6 more
doaj   +1 more source

5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems.

open access: yesPLoS ONE, 2020
Firefly luciferase-based ATP detection assays are frequently used as a sensitive, cost-efficient method for monitoring hygiene in many industrial settings.
Ce Shi   +9 more
doaj   +1 more source

Adeno‐associated virus serotype 2 capsid variants for improved liver‐directed gene therapy

open access: yesHepatology, EarlyView., 2022
Abstract Background and Aims Current liver‐directed gene therapies look for adeno‐associated virus (AAV) vectors with improved efficacy. With this background, capsid engineering is explored. Whereas shuffled capsid library screenings have resulted in potent liver targeting variants with one first vector in human clinical trials, modifying natural ...
Nadja Meumann   +25 more
wiley   +1 more source

The location and nature of general anesthetic binding sites on the active conformation of firefly luciferase; a time resolved photolabeling study. [PDF]

open access: yesPLoS ONE, 2012
Firefly luciferase is one of the few soluble proteins that is acted upon by a wide variety of general anesthetics and alcohols; they inhibit the ATP-driven production of light.
Sivananthaperumal Shanmugasundararaj   +7 more
doaj   +1 more source

Basement membrane-rich Organoids with functional human blood vessels are permissive niches for human breast cancer metastasis [PDF]

open access: yes, 2013
Metastasic breast cancer is the leading cause of death by malignancy in women worldwide. Tumor metastasis is a multistep process encompassing local invasion of cancer cells at primary tumor site, intravasation into the blood vessel, survival in systemic ...
Alonso-Camino, Vanesa   +12 more
core   +7 more sources

Genomic and experimental data provide new insights into luciferin biosynthesis and bioluminescence evolution in fireflies

open access: yesScientific Reports, 2020
Fireflies are among the most charismatic insects for their spectacular bioluminescence, but the origin and evolution of bioluminescence remain elusive.
Ru Zhang   +23 more
doaj   +1 more source

Using bioluminescence to image gene expression and spontaneous behavior in freely moving mice.

open access: yesPLoS ONE, 2023
Bioluminescence imaging (BLI) of gene expression in live animals is a powerful method for monitoring development, tumor growth, infections, healing, and other progressive, long-term biological processes. BLI remains an effective approach for reducing the
Astha Malik   +2 more
doaj   +1 more source

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