A dual-color far-red to near-infrared firefly luciferin analogue designed for multiparametric bioluminescence imaging. [PDF]
Angew Chem Int Ed Engl, 2014 Red-shifted bioluminescent emitters allow improved in vivo tissue penetration and signal quantification, and have led to the development of beetle luciferin analogues that elicit red-shifted bioluminescence with firefly luciferase.
Jathoul AP +3 more
europepmc +2 more sources
Mathematical Model of the Firefly Luciferase Complementation Assay Reveals a Non-Linear Relationship between the Detected Luminescence and the Affinity of the Protein Pair Being Analyzed. [PDF]
PLoS ONE, 2016 The firefly luciferase complementation assay is widely used as a bioluminescent reporter technology to detect protein-protein interactions in vitro, in cellulo, and in vivo.
Renee Dale +3 more
doaj +1 more source
Immobilization of Firefly Bioluminescent System: Development and Application of Reagents
Biosensors, 2022 The present study describes the method of preparing reagents containing firefly luciferase (FLuc) and its substrate, D-luciferin, immobilized into gelatin gel separately or together.
Elena N. Esimbekova +2 more
doaj +1 more source
Adeno‐associated virus serotype 2 capsid variants for improved liver‐directed gene therapy
Hepatology, EarlyView., 2022 Abstract Background and Aims Current liver‐directed gene therapies look for adeno‐associated virus (AAV) vectors with improved efficacy. With this background, capsid engineering is explored. Whereas shuffled capsid library screenings have resulted in potent liver targeting variants with one first vector in human clinical trials, modifying natural ...
Nadja Meumann +25 more
wiley +1 more source
Frontiers in Bioengineering and Biotechnology, 2021 Immunoassays are widely used for detection of antibodies against specific antigens in diagnosis, as well as in electrophoretic techniques such as Western Blotting.
Vadim R. Viviani +4 more
doaj +1 more source
Frontiers in Bioengineering and Biotechnology, 2021 The protein–protein interaction assay is a key technology in various fields, being applicable in drug screening as well as in diagnosis and inspection, wherein the stability of assays is important.
Yuki Ohmuro-Matsuyama +6 more
doaj +1 more source
, 2023 Polyacrylamide (PAM) hydrogels are extensively used as extracellular matrix mimics to study specific cell-materials interactions. However, chemistries typically applied for biofunctionalization of PAM lack chemo-selectivity and control over ligand ...
Jeroen, Leijten +6 more
core +1 more source
5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems.
PLoS ONE, 2020 Firefly luciferase-based ATP detection assays are frequently used as a sensitive, cost-efficient method for monitoring hygiene in many industrial settings.
Ce Shi +9 more
doaj +1 more source
The location and nature of general anesthetic binding sites on the active conformation of firefly luciferase; a time resolved photolabeling study. [PDF]
PLoS ONE, 2012 Firefly luciferase is one of the few soluble proteins that is acted upon by a wide variety of general anesthetics and alcohols; they inhibit the ATP-driven production of light.
Sivananthaperumal Shanmugasundararaj +7 more
doaj +1 more source
The nuclear factor κB inhibitor (E)-2-fluoro-4′-methoxystilbene inhibits firefly luciferase
Bioscience Reports, 2012 Photinus pyralis (firefly) luciferase is widely used as a reporter system to monitor alterations in gene promoter and/or signalling pathway activities in vitro.
Albert Braeuning, Silvia Vetter
doaj +1 more source