A practical, biomimetic, one-pot synthesis of firefly luciferin [PDF]
Scientific ReportsThe bioluminescence reaction of firefly luciferase with D-luciferin has become an indispensable imaging technique in modern biology and life science experiments, but the high cost of D-luciferin is limiting its further application.
Maria Kato +4 more
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Biosensing firefly luciferin synthesis in bacteria reveals a cysteine-dependent quinone detoxification route in Coleoptera [PDF]
Scientific Reports, 2022 Luciferin biosynthetic origin and alternative biological functions during the evolution of beetles remain unknown. We have set up a bioluminescent sensing method for luciferin synthesis from cysteine and benzoquinone using E.
Daniel Rangel de Souza +3 more
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How to Select Firefly Luciferin Analogues for In Vivo Imaging. [PDF]
Int J Mol Sci, 2021 Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models.
Saito-Moriya R +6 more
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Biosynthesis of firefly luciferin in adult lantern: decarboxylation of L-cysteine is a key step for benzothiazole ring formation in firefly luciferin synthesis. [PDF]
PLoS ONE, 2013 Bioluminescence in fireflies and click beetles is produced by a luciferase-luciferin reaction. The luminescence property and protein structure of firefly luciferase have been investigated, and its cDNA has been used for various assay systems.
Yuichi Oba +4 more
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Correction: Biosynthesis of Firefly Luciferin in Adult Lantern: Decarboxylation of L-Cysteine is a Key Step for Benzothiazole Ring Formation in Firefly Luciferin Synthesis [PDF]
PLoS ONE, 2014 The PLOS ONE Staff
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Firefly luciferase can use L-luciferin to produce light [PDF]
Biochemical Journal, 1996 l-Luciferin is a competitive inhibitor of firefly luciferase with a Ki between 3 and 4 μM. Furthermore l-luciferin can serve as an alternative substrate for light production. Catalysis of l-luciferin can be observed in the absence of, or at low concentrations of, d-luciferin.
Nicolas Lembert
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Regulatory effects of ATP and luciferin on firefly luciferase activity [PDF]
Biochemical Journal, 1995 ATP and luciferin are not only substrates of firefly luciferase, but can, in addition, modulate its activity. High concentrations of luciferin induce a conformational change of the enzyme that temporarily reduces the catalytic rate. Re-activation takes approx.
Nicolas Lembert, L Å Idahl
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A QM/MM Study on the Initiation Reaction of Firefly Bioluminescence—Enzymatic Oxidation of Luciferin [PDF]
Molecules, 2021 Among all bioluminescent organisms, the firefly is the most famous, with a high luminescent efficiency of 41%, which is widely used in the fields of biotechnology, biomedicine and so on.
Mohan Yu, Yajun Liu
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Important Site of Luciferin on Firefly Bioluminescence [PDF]
ECS Transactions, 2008 The firefly bioluminescence is well known in the world. Although its emission efficiency is assumed the best of the earth, a color variation is aspired in top science. For producing color variation, we have innovated new technology by analyzing biofunctions.
Shôjirô Maki
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Luciferase expression and bioluminescence does not affect tumor cell growth
Molecular Cancer, 2010 Live animal imaging is becoming an increasingly common technique for accurate and quantitative assessment of tumor burden over time. Bioluminescence imaging systems rely on a bioluminescent signal from tumor cells, typically generated from expression of ...
Rasko John EJ +4 more
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