Biosynthesis of firefly luciferin in adult lantern: decarboxylation of L-cysteine is a key step for benzothiazole ring formation in firefly luciferin synthesis. [PDF]
PLoS ONE, 2013 Bioluminescence in fireflies and click beetles is produced by a luciferase-luciferin reaction. The luminescence property and protein structure of firefly luciferase have been investigated, and its cDNA has been used for various assay systems.
Yuichi Oba +4 more
doaj +6 more sources
A practical, biomimetic, one-pot synthesis of firefly luciferin [PDF]
Scientific ReportsThe bioluminescence reaction of firefly luciferase with D-luciferin has become an indispensable imaging technique in modern biology and life science experiments, but the high cost of D-luciferin is limiting its further application.
Maria Kato +4 more
doaj +5 more sources
Correction: Biosynthesis of Firefly Luciferin in Adult Lantern: Decarboxylation of L-Cysteine is a Key Step for Benzothiazole Ring Formation in Firefly Luciferin Synthesis [PDF]
PLoS ONE, 2014 Correction: Biosynthesis of Firefly Luciferin in Adult Lantern: Decarboxylation of L-Cysteine is a Key Step for Benzothiazole Ring Formation in Firefly Luciferin ...
PLOS ONE (2189197)
doaj +7 more sources
How to Select Firefly Luciferin Analogues for In Vivo Imaging. [PDF]
Int J Mol Sci, 2021 Bioluminescence reactions are widely applied in optical in vivo imaging in the life science and medical fields. Such reactions produce light upon the oxidation of a luciferin (substrate) catalyzed by a luciferase (enzyme), and this bioluminescence enables the quantification of tumor cells and gene expression in animal models.
Saito-Moriya R +6 more
europepmc +5 more sources
Biosensing firefly luciferin synthesis in bacteria reveals a cysteine-dependent quinone detoxification route in Coleoptera [PDF]
Scientific Reports, 2022 Luciferin biosynthetic origin and alternative biological functions during the evolution of beetles remain unknown. We have set up a bioluminescent sensing method for luciferin synthesis from cysteine and benzoquinone using E.
Daniel Rangel de Souza +3 more
doaj +2 more sources
Transcriptome analysis reveals candidate genes involved in luciferin metabolism in Luciola aquatilis (Coleoptera: Lampyridae) [PDF]
PeerJ, 2016 Bioluminescence, which living organisms such as fireflies emit light, has been studied extensively for over half a century. This intriguing reaction, having its origins in nature where glowing insects can signal things such as attraction or defense, is ...
Wanwipa Vongsangnak +2 more
doaj +3 more sources
Scientific Reports, 2018 The New Zealand glowworm, Arachnocampa luminosa, is well-known for displays of blue-green bioluminescence, but details of its bioluminescent chemistry have been elusive.
Oliver C. Watkins +3 more
doaj +2 more sources
A QM/MM Study on the Initiation Reaction of Firefly Bioluminescence—Enzymatic Oxidation of Luciferin
Molecules, 2021 Among all bioluminescent organisms, the firefly is the most famous, with a high luminescent efficiency of 41%, which is widely used in the fields of biotechnology, biomedicine and so on.
Ya-Jun Liu, Liu Yajun
exaly +3 more sources
Bioluminescent Mycobacterium abscessus in Galleria mellonella: a preclinical screening tool for antimycobacterial compounds [PDF]
Frontiers in Cellular and Infection MicrobiologyMycobacterium abscessus (Mab) is an emerging pulmonary pathogen with extensive drug resistance, resulting in prolonged multidrug therapy, severe side effects, and cure rates below 50%.
Tine Van Win +7 more
doaj +2 more sources
A Bioluminescent Reporter System for Real-Time Monitoring of the Unfolded Protein Response in Plants. [PDF]
Plant Biotechnol JABSTRACT The unfolded protein response (UPR) is a critical mechanism for maintaining endoplasmic reticulum (ER) homeostasis under stress. Here, we developed a bioluminescent reporter system, AtbZIP60‐LUC, in Arabidopsis to dynamically monitor ER stress by coupling IRE1‐mediated splicing of bZIP60 mRNA to firefly luciferase (LUC) expression.
Liu Y +12 more
europepmc +2 more sources