Results 81 to 90 of about 21,179 (270)

The BnTFL1‐BnJAM3‐BnSWEETs Module Orchestrates Seed Storage Reserve Accumulation in Brassica napus

Advanced Science, EarlyView.
BnaC03.TFL1 and BnaA01.JAM3 form a complex that facilitates the transport of soluble sugars from the seed coat to the embryo by directly activating the expression of BnSWEETs. Furthermore, BnaC03.TFL1 and BnaA01.JAM3 work cooperatively to promote soluble sugar and oil production while suppressing protein deposition.
Jianjun Wang, Zijin Liu, Minshan Jin, Junting Liu, Shihao Wei, Dengmao Yang, Saiqi Yang, Yuan Guo, Mingxun Chen   +8 more
wiley   +1 more source

In vivo imaging of mice infected with bioluminescent Trypanosoma cruzi unveils novel sites of infection

Parasites & Vectors, 2014
Background The development of techniques that allow the imaging of animals infected with parasites expressing luciferase opens up new possibilities for following the fate of parasites in infected mammals. Methods D-luciferin potassium salt stock solution
Cristina Henriques, Andréa Henriques-Pons, Marcelo Meuser-Batista, Aline Salgado Ribeiro, Wanderley de Souza   +4 more
doaj   +1 more source

Triple Bioluminescence Imaging for In Vivo Monitoring of Cellular Processes

Molecular Therapy: Nucleic Acids, 2013
Bioluminescence imaging (BLI) has shown to be crucial for monitoring in vivo biological processes. So far, only dual bioluminescence imaging using firefly (Fluc) and Renilla or Gaussia (Gluc) luciferase has been achieved due to the lack of availability ...
Casey A Maguire, M Sarah Bovenberg, Matheus HW Crommentuijn, Johanna M Niers, Mariam Kerami, Jian Teng, Miguel Sena-Esteves, Christian E Badr, Bakhos A Tannous   +8 more
doaj   +1 more source

pFAR plasmids: New Eukaryotic Expression Vectors for Gene Therapy, devoid of Antibiotic Resistance Markers [PDF]

, 2008
Efficient production of eukaryotic expression vectors requires the selection of plasmid-containing bacteria. To avoid the risk of dissemination of antibiotic resistance markers, we developed a new system to produce a family of plasmids Free of Antibiotic
Corinne Marie, Daniel Scherman, Gaelle Vandermeulen, Magali Richard, Mickael Quiviger, Veronique Preat   +5 more
core   +1 more source

Programmable probiotics for detection of cancer in urine [PDF]

, 2014
Rapid advances in the forward engineering of genetic circuitry in living cells has positioned synthetic biology as a potential means to solve numerous biomedical problems, including disease diagnosis and therapy.
Allen, Kaitlin N., Bhatia, Sangeeta N, Danino, Tal, Hasty, Jeff, Kwong, Gabriel A., Li, Howard, Prindle, Arthur, Skalak, Matthew T.   +7 more
core   +1 more source

OsGSK2‐OsTCP19 Module Integrates Nitrogen and Brassinosteroid Signaling to Regulate Nitrogen Utilization and Root Growth in Rice

Advanced Science, EarlyView.
OsGSK2‐OsTCP19 module regulating nitrate response and lateral root (LR) development. Low‐nitrate condition results in reduced BR response, accumulation of OsGSK2 and phosphorylated OsTCP19, which suppresses the expression of nitrate‐responsive genes and LR‐development genes and impairs rice growth.
Yongqiang Liu, Weiwei Li, Xiaohui Ma, Xiaohan Wang, Yarong Shi, Xiahe Huang, Xinran Li, Yingchun Wang, Hongning Tong, Bin Hu, Wenbin Zhou, Chengcai Chu   +11 more
wiley   +1 more source

Enhanced Intracellular Stability and Translation Efficiency of mRNA Drugs by a 2‐arm mRNA Platform

Advanced Science, EarlyView.
We constructed a 2‐arm mRNA, characterized by a unique topology formed through the dimerization of two mRNA 3’ tails. The 2‐arm mRNA improves 3’ tail stability and resistance to nuclease degradation, resulting in an intracellular half‐life of up to 65 h. This method substantially enhances the translation capacity of mRNA drugs.
Xucong Teng, Jiahao Lin, Qiushuang Zhang, Xiangdong Zhang, Yicong Dai, Jinghong Li   +5 more
wiley   +1 more source

Analytical Applications of Bioluminescence and Chemiluminescence [PDF]

Bioluminescence and chemiluminescence studies were used to measure the amount of adenosine triphosphate and therefore the amount of energy available. Firefly luciferase - luciferin enzyme system was emphasized.
Chappelle, E. W., Picciolo, G. L.
core   +1 more source

Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging [PDF]

, 2018
The sensitivity of bioluminescence imaging in animals is primarily dependent on the amount of photons emitted by the luciferase enzyme at wavelengths greater than 620 nm where tissue penetration is high.
Encell, L.P. (Lance P.), Hall, M.P. (Mary P.), Kirkland, T.A. (Thomas A.), Löwik, C.W.G.M. (Clemens), Mezzanotte, L. (Laura), Que, I. (Ivo), Ridwan, R.Y. (Yanto), Root, M. (Moniek) van 't, Shi, C. (Ce), Wood, K.V. (Keith V.), Wood, M.G. (Monika G.), Woodroofe, C.C. (Carolyn C.)   +11 more
core   +1 more source

Improved methods for counting bacteria in physiological fluids [PDF]

, 1974
Bacterial population detection is based on detection of adenosine triphosphate (ATP), chemical present in all living matter. Amount of ATP in sample, after chemically removing all nonbacterial ATP, is directly related to bacterial population. Sensitivity
Picciolo, G. L.
core   +1 more source