Results 81 to 90 of about 14,327 (227)

Triple Bioluminescence Imaging for In Vivo Monitoring of Cellular Processes

Molecular Therapy: Nucleic Acids, 2013
Bioluminescence imaging (BLI) has shown to be crucial for monitoring in vivo biological processes. So far, only dual bioluminescence imaging using firefly (Fluc) and Renilla or Gaussia (Gluc) luciferase has been achieved due to the lack of availability ...
Casey A Maguire, M Sarah Bovenberg, Matheus HW Crommentuijn, Johanna M Niers, Mariam Kerami, Jian Teng, Miguel Sena-Esteves, Christian E Badr, Bakhos A Tannous   +8 more
doaj   +1 more source

SlMED25‐SlPHR3‐SlSPX2 module fine‐tunes SlPHR3‐mediated transcriptional activation of phosphate starvation response in tomato

Journal of Integrative Plant Biology, EarlyView.
The Mediator subunit SlMED25 and the co‐repressor SlSPX2 competitively bind the N‐terminal domain of the phosphate starvation response transcription factor SlPHR3 in tomato to form a sensitive molecular switch, which dynamically modulates phosphate starvation responses and maintains phosphate homeostasis, offering valuable targets for breeding low ...
Mingtong Zhai, Hongyu Han, Yu Zhang, Yunfeng Zhao, Hongying Guo, Hui Wang, Chuanlong Sun, Xianwen Meng, Lei Deng, Qian Chen, Chuanyou Li   +10 more
wiley   +1 more source

Strategy to study on the biosynthetic pathway of firefly luciferin in an adult lantern of a living firefly by injecting the stable isotope-labeled compounds, and the bioluminescence reaction catalyzed by firefly/beetle luciferase.

, 2013
A. Proposed biosynthetic pathway of firefly luciferin (I) from p-benzoquinone and two L-cysteines in an adult lantern, and the luminescence reaction of luciferin with firefly luciferase, followed by the formation of 2-cyano-6-hydroxybenzothiazole (III ...
Naoki Yoshida (504407), Shusei Kanie (504408), Makoto Ojika (504409), Satoshi Inouye (203922), Yuichi Oba (504406)   +4 more
core   +1 more source

Uncovering the role of the PPR protein PHOTOSYSTEM ONE BIOGENESIS FACTOR6 in splicing chloroplast group II introns

Journal of Integrative Plant Biology, EarlyView.
The P‐class pentatricopeptide repeat (PPR) protein PHOTOSYSTEM ONE BIOGENESIS FACTOR (PBF6) forms splicing complexes with other known splicing factors to facilitate chloroplast intron splicing. PBF6 cooperates with other PPR splicing factors to promote the splicing of the same intron through forming respective splicing complexes.
Mengyu Li, Mengwei Sun, Jinlian Lv, Mingming Song, Siyu Lu, Aihong Zhang, Congming Lu   +6 more
wiley   +1 more source

LURP1, a SARD1‐regulated protein, coordinates with the helper NLR ADR1 to promote plant immunity

New Phytologist, EarlyView.
The working model of LURP1 function in plant immunity. Upon pathogen infection, SARD1/CBP60g promotes the transcription of LURP1 directly. LURP1 likely functions as an adaptor protein that associates with the PRRs–BAK1 complexes at the plasma membrane (PM) while also interacts with the ADR1 family helper NLRs.
Xueru Liu, Weijie Huang, Zhengxi Gong, Chenying Li, Siyu Chen, Shucai Wang, Yuelin Zhang, Xin Li   +7 more
wiley   +1 more source

The distribution of ATP within tomato (Lycopersicon esculentum Mill.) embryos correlates with germination whee as total ATP concentration does not

, 2002
The distribution of ATP in tomato seeds was visualized by monitoring the luminescence of frozen sections on top of a gel containing all the components of the luciferase reaction, but excluding ATP.
Joosen, R.V.L., Hilhorst, H.W.M., Spoelstra, P.   +2 more
core   +1 more source

Membrane-permeable luciferin esters for assay of firefly luciferase in live intact cells

, 1991
Five esters of luciferin were synthesized and compared with native luciferin as substrates for firefly luciferase expressed in live intact mammalian cells.
Watmore, D., White, M. R H, Craig, F. F., McCapra, F., Simmonds, A. C.   +4 more
core   +1 more source

Mapping and Functional Characterization of Homologous Genes AhSUCA06 and AhSUCA16 Underlying Sucrose, Oil and Protein Contents in Peanut (Arachis hypogaea L.)

Plant Biotechnology Journal, EarlyView.
ABSTRACT Cultivated peanut (Arachis hypogaea L.) is an important oilseed and cash crop, and seed sucrose content (SSC), seed oil content (SOC) and seed protein content (SPC) are key determinants of seed flavour, texture, and overall quality. Identifying quantitative trait loci (QTLs) and candidate genes associated with SSC, SOC and SPC is therefore of ...
Yuzhen Zheng, Feiyan Qi, Lei Shi, Ziqi Sun, Hongfei Liu, Hua Liu, Li Qin, Juan Wang, Chenyang Zhi, Mengmeng Wang, Ziqiang Mo, Stefano Pavan, Xiao Wang, Yaojun Hu, Huanhuan Zhao, Xiaona Li, Wenzhao Dong, Meng Zhang, XiaoDong Dai, Zheng Zheng, Xinyou Zhang   +20 more
wiley   +1 more source

Enhancing tissue regeneration studies with luciferase-transgenic rats and bioluminescence imaging: A comprehensive review

Chinese Journal of Plastic and Reconstructive Surgery
Bioluminescence imaging (BLI) in rodent models has revolutionized preclinical research in recent decades, enabling precise and noninvasive observation of cellular and molecular processes in vivo.
Poh-Ching Tan, Eiji Kobayashi, Qingfeng Li, Shuangbai Zhou   +3 more
doaj   +1 more source

A Dual-Color Bioluminescence Reporter Mouse for Simultaneous in vivo Imaging of T Cell Localization and Function

Frontiers in Immunology, 2019
Non-invasive imaging technologies to visualize the location and functionality of T cells are of great value in immunology. Here, we describe the design and generation of a transgenic mouse in which all T cells constitutively express green-emitting click ...
Jan Willem Kleinovink, Laura Mezzanotte, Laura Mezzanotte, Giorgia Zambito, Giorgia Zambito, Giorgia Zambito, Marieke F. Fransen, Luis J. Cruz, J. Sjef Verbeek, Alan Chan, Ferry Ossendorp, Clemens Löwik, Clemens Löwik, Clemens Löwik   +13 more
doaj   +1 more source