Basic methods for fission yeast [PDF]
AbstractThe fission yeast Schizosaccharomyces pombe is a popular model system, and has been particularly influential in studies of the cell cycle and chromosome dynamics. Despite its differences from Saccharomyces cerevisiae, the tools and methods for fission yeast are conceptually similar to those used in budding yeast.
Susan L. Forsburg, Nicholas Rhind
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Double-strand break repair and homologous recombination in Schizosaccharomyces pombe [PDF]
In recent years our understanding of double strand break repair and homologous recombination in Schizosaccharomyces pombe has increased significantly, and the identification of novel pathways and genes with homologues in higher eukaryotes has increased ...
Ahmad+102 more
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DNA checkpoints in fission yeast [PDF]
The DNA integrity checkpoints ensure completion of DNA replication and DNA repair before entry into mitosis ([O'Connell et al., 2000][1]). Unreplicated DNA or unrepaired DNA will cause catastrophic chromosome segregation.
Antony M. Carr, Kanji Furuya
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Splitting of the fission yeast septum [PDF]
In cell-walled organisms, a cross wall (septum) is produced during cytokinesis, which then splits in certain organisms to allow the daughter cells to separate. The formation and the subsequent cleavage of the septum require wall synthesis and wall degradation, which need to be strictly coordinated in order to prevent cell lysis.
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Analysis of DNA Metabolism in Fission Yeast [PDF]
The fission yeast Schizosaccharomyces pombe is an excellent model organism to study DNA metabolism, in which the DNA replication and repair mechanisms are evolutionarily conserved. In this introduction we describe a range of methods commonly used to study aspects of DNA metabolism in fission yeast, focusing on approaches used for the analysis of genome
Antequera, Francisco+1 more
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Altering the stability of the Cdc8 overlap region modulates the ability of this tropomyosin to bind cooperatively to actin and regulate myosin. [PDF]
Tropomyosin (Tm) is an evolutionarily conserved ?-helical coiled-coil protein, dimers of which form end-to-end polymers capable of associating with and stabilising actin-filaments and regulate myosin function. The fission yeast, Schizosaccharomyces pombe,
East, Daniel A.+5 more
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Fission Yeast Cell Wall Analysis [PDF]
The Schizosaccharomyces pombe cell wall is a rigid exoskeletal structure mainly composed of interlinked glucose polysaccharides and galactomannoproteins. It is essential for survival of the fission yeast, as it prevents cells from bursting from internal turgor pressure and protects them from mechanical injuries.
Pérez, Pilar, Ribas, Juan Carlos
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Regulation of Ace2-dependent genes requires components of the PBF complex in schizosaccharomyces pombe [PDF]
The division cycle of unicellular yeasts is completed with the activation of a cell separation program that results in the dissolution of the septum assembled during cytokinesis between the 2 daughter cells, allowing them to become independent entities ...
Alonso-Nuñez, María Luisa+4 more
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The role of dynamin-related proteins in vacuole biogenesis in fission yeast (Schizosaccharomyces pombe) : a thesis presented in partial fulfillment of the requirements for the degree of Master of Science in Biochemistry at Massey University, Palmerston North, New Zealand [PDF]
Dynamins are GTPases concerned with membrane tubulation and scission (Praefcke and McMahon, 2004). In the fission yeast, Schizosaccharomyces pombe, the dynamin-related proteins (DRPs) Vps1 and Dnm1 act redundantly in peroxisome biogenesis (Jourdain et al.
Röthlisberger, Sarah Ruth
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Quantitative single-molecule microscopy reveals that CENP-A(Cnp1) deposition occurs during G2 in fission yeast [PDF]
The inheritance of the histone H3 variant CENP-A in nucleosomes at centromeres following DNA replication is mediated by an epigenetic mechanism. To understand the process of epigenetic inheritance, or propagation of histones and histone variants, as ...
Allshire, Robin C+11 more
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