A discrete class of intergenic DNA dictates meiotic DNA break hotspots in fission yeast.
PLoS Genetics, 2007 Meiotic recombination is initiated by DNA double-strand breaks (DSBs) made by Spo11 (Rec12 in fission yeast), which becomes covalently linked to the DSB ends.
Gareth A Cromie +5 more
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Plasticity of fission yeast CENP-A chromatin driven by relative levels of histone H3 and H4. [PDF]
PLoS Genetics, 2007 The histone H3 variant CENP-A assembles into chromatin exclusively at centromeres. The process of CENP-A chromatin assembly is epigenetically regulated.
Araceli G Castillo +6 more
doaj +1 more source
Functional mapping of the fission yeast DNA polymerase delta B-subunit Cdc1 by site-directed and random pentapeptide insertion mutagenesis [PDF]
, 2009 JSG was supported by a BBSRC Research Committee studentship, EVK by the Darwin Trust of Edinburgh, and FCG and SAM by a Wellcome Trust Senior Research Fellowship in Basic Biomedical Sciences.
Garcia, JS +5 more
core +1 more source
Identification of a small RhoA GTPase inhibitor effective in fission yeast and human cells
Open Biology, 2023 The Rho GTPase family proteins are key regulators of cytoskeletal dynamics. Deregulated activity of Rho GTPases is associated with cancers and neurodegenerative diseases, and their potential as drug targets has long been recognized. Using an economically
Jun Morishita, Paul Nurse
doaj +1 more source
Journal of Molecular Biology, 2017 R-loops, which result from the formation of stable DNA:RNA hybrids, can both threaten genome integrity and act as physiological regulators of gene expression and chromatin patterning.
Stella R. Hartono +5 more
semanticscholar +1 more source
Latrunculin A delays anaphase onset in fission yeast by disrupting an ase1-independent pathway controlling mitotic spindle stability [PDF]
, 2008 It has been proposed previously that latrunculin A, an inhibitor of actin polymerization, delays the onset of anaphase by causing spindle misorientation in fission yeast.
Meadows, John C., Millar, Jonathan B. A.
core +1 more source
Eic1 links Mis18 with the CCAN/Mis6/Ctf19 complex to promote CENP-A assembly [PDF]
Open Biology, 2014 CENP-A chromatin forms the foundation for kinetochore assembly. Replication-independent incorporation of CENP-A at centromeres depends on its chaperone HJURPScm3, and Mis18 in vertebrates and fission yeast.
Lakxmi Subramanian +3 more
doaj +1 more source
eLife, 2021 Mammalian target of rapamycin complex 1 (TORC1) is controlled by the GATOR complex composed of the GATOR1 subcomplex and its inhibitor, the GATOR2 subcomplex, sensitive to amino acid starvation.
Tomoyuki Fukuda +9 more
doaj +1 more source
Bub1 is a fission yeast kinetochore scaffold protein, and is sufficient to recruit other spindle checkpoint proteins to ectopic sites on chromosomes [PDF]
, 2007 The spindle checkpoint delays anaphase onset until all chromosomes have attached in a bi-polar manner to the mitotic spindle. Mad and Bub proteins are recruited to unattached kinetochores, and generate diffusible anaphase inhibitors.
Rischitor, Patricia E +8 more
core +1 more source
DNA checkpoints in fission yeast [PDF]
Journal of Cell Science, 2003 The DNA integrity checkpoints ensure completion of DNA replication and DNA repair before entry into mitosis ([O'Connell et al., 2000][1]). Unreplicated DNA or unrepaired DNA will cause catastrophic chromosome segregation.
Kanji, Furuya, Antony M, Carr
openaire +2 more sources