Activation of Ciona sperm motility: phosphorylation of dynein polypeptides and effects of a tyrosine kinase inhibitor [PDF]
A high molecular mass dynein ATPase polypeptide and a 18–20 kDa dynein light chain of Ciona sperm flagella are phosphorylated during in vivo activation of motility or in vitro activation of motility by incubation with cyclic AMP.
Brokaw, Charles J., Dey, Chinmoy S.
core
This study describes a novel species of marine heterotrophic nanoflagellate, Hexatilemonas jangsaensis gen. et sp. n., within the uncultured APU‐30 clade, and highlights the global distribution pattern of this previously uncharacterised genus. This isolate is hypothesised to feed on bacteria attached to substrates in the water column using pseudopodia.
Dong Hyuk Jeong +4 more
wiley +1 more source
Motility of triton-demembranated sea urchin sperm flagella during digestion by trypsin [PDF]
The survival curves for a population of reactivated spermatozoa exposed to digestion by trypsin indicate that a large number of trypsin-sensitive targets must be digested before the flagellum disintegrates.
Brokaw, C. J., Simonick, T. F.
core +2 more sources
Chemotaxis of Arbacia punctulata spermatozoa to resact, a peptide from the egg jelly layer [PDF]
Resact, a peptide of known sequence isolated from the jelly layer of Arbacia punctulata eggs, is a potent chemoattractant for A. punctulata spermatozoa. The chemotactic response is concentration dependent, is abolished by pretreatment of the spermatozoa ...
Brokaw, Charles J. +3 more
core +3 more sources
Microtubule sliding in swimming sperm flagella: direct and indirect measurements on sea urchin and tunicate spermatozoa [PDF]
Direct measurements of microtubule sliding in the flagella of actively swimming, demembranated, spermatozoa have been made using submicron diameter gold beads as markers on the exposed outer doublet microtubules.
Brokaw, Charles J.
core
Maintenance of Constant Wave Parameters by Sperm Flagella at Reduced Frequencies of Beat [PDF]
1. Treatment of Ciona spermatozoa with low concentrations of Triton X-100 (less than 0·01 %) causes them to beat at lower than normal frequencies. The wavelength of the flagellar bending waves remains constant over the range from 10 to 40 Hz.
Brokaw, C. J., Josslin, R.
core
DPY-30 Domain and its Flanking Sequence Mediate the Assembly Modulation of Flagellar Radial Spoke Complexes [PDF]
RIIa is known as the dimerization and docking (D/D) domain of the cyclic AMP (cAMP)-dependent protein kinase. However, numerous molecules, including radial spoke protein 2 (RSP2) in Chlamydomonas flagella, also contain an RIIa or a similar DPY-30 domain.
Foster, Kenneth W. +2 more
core +1 more source
Radial Spoke Proteins of \u3cem\u3eChlamydomonas\u3c/em\u3e Flagella [PDF]
The radial spoke is a ubiquitous component of `9+2\u27 cilia and flagella, and plays an essential role in the control of dynein arm activity by relaying signals from the central pair of microtubules to the arms. The Chlamydomonas reinhardtii radial spoke
Agrin, Nathan S. +11 more
core +1 more source
Radial Spokes-A Snapshot of the Motility Regulation, Assembly, and Evolution of Cilia and Flagella [PDF]
Propulsive forces generated by cilia and flagella are used in events that are critical for the thriving of diverse eukaryotic organisms in their environments. Despite distinctive strokes and regulations, the majority of them adopt the 9+2 axoneme that is
Liu, Yi, Yang, Pinfen, Zhu, Xiaoyan
core +1 more source
Cofactor Fingerprinting with STD NMR to Characterize Proteins of Unknown Function: Identification of a Rare cCMP Cofactor Preference [PDF]
Proteomics efforts have created a need for better strategies to functionally categorize newly discovered proteins. To this end, we have employed saturation transfer difference NMR with pools of closely related cofactors, to determine cofactor preferences.
Sem, Daniel, Yao, Huili
core +1 more source

