A fluorescence sensor for nitroreductase detection in hypoxic cells and zebrafish
Results in Chemistry, 2021 Nitroreductase (NTR) is a type of cytoplasmic enzyme that depends on flavin mononucleotide or flavin adenine dinucleotide, and that is widely present in tumor cells where it increases during hypoxia. NTR can effectively reduce aromatic nitro compounds to
Wan Sun +7 more
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Enhanced small green fluorescent proteins as a multisensing platform for biosensor development
Frontiers in Bioengineering and Biotechnology, 2022 Engineered light, oxygen, and voltage (LOV)-based proteins are able to fluoresce without oxygen requirement due to the autocatalytic incorporation of exogenous flavin as a chromophore thus allowing for live cell imaging under hypoxic and anaerobic ...
Guo-Teng Liang +25 more
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Crystal Structures of Putative Flavin Dependent Monooxygenase from Alicyclobacillus Acidocaldarius
Crystals, 2019 Flavin dependent monooxygenases catalyze various reactions to play a key role in biological processes, such as catabolism, detoxification, and biosynthesis.
Hyunjin Moon +2 more
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H2O2 selectively damages the binuclear iron-sulfur cluster N1b of respiratory complex I
Scientific Reports, 2023 NADH:ubiquinone oxidoreductase, respiratory complex I, plays a major role in cellular energy metabolism by coupling electron transfer with proton translocation.
Lisa Strotmann +6 more
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FMN reduces Amyloid-β toxicity in yeast by regulating redox status and cellular metabolism
Nature Communications, 2020 Saccharomyces cerevisiae is a model organism to study proteins involved in neurodegeneration. Here, the authors performed a yeast genome-wide synthetic genetic interaction array (SGA) to screen for toxicity modifiers of Aβ42 and identify riboflavin ...
Xin Chen +6 more
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Mass spectrometry locates local and allosteric conformational changes that occur on cofactor binding
Nature Communications, 2016 The decarboxylate enzyme Fdc1 requires a prenylated flavin mononucleotide co-factor for activity. Here, the authors use a variety of mass spectrometric techniques to observe the structural changes in this protein in response to co-factor binding.
Rebecca Beveridge +5 more
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Co-Overexpression of RIB1 and RIB6 Increases Riboflavin Production in the Yeast Candida famata
Fermentation, 2022 Riboflavin or vitamin B2 is a water-soluble vitamin and a precursor of flavin coenzymes, flavin mononucleotide, and flavin adenine dinucleotide, which play a key role as enzyme cofactors in energy metabolism.
Yana Petrovska +4 more
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Frontiers in Oncology, 2023 PurposeModern techniques for improved tumor visualization have the aim to maximize the extent of resection during brain tumor surgery and thus improve patient prognosis.
David Reichert +18 more
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Production of Vitamin B2 (Riboflavin) by Microorganisms: An Overview
Frontiers in Bioengineering and Biotechnology, 2020 Riboflavin is a crucial micronutrient that is a precursor to coenzymes flavin mononucleotide and flavin adenine dinucleotide, and it is required for biochemical reactions in all living cells.
Liudmila A. Averianova +8 more
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The structural and functional basis of catalysis mediated by NAD(P)H:acceptor Oxidoreductase (FerB) of Paracoccus denitrificans. [PDF]
PLoS ONE, 2014 FerB from Paracoccus denitrificans is a soluble cytoplasmic flavoprotein that accepts redox equivalents from NADH or NADPH and transfers them to various acceptors such as quinones, ferric complexes and chromate.
Vojtěch Sedláček +3 more
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