Journal of Fungi, 2023 Riboflavin (RF), or vitamin B2, is an essential compound for yeast growth and a precursor of the flavin coenzymes, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), involved in redox and non-redox processes.
Alessandra Di Canito +6 more
doaj +1 more source
A fluorescence sensor for nitroreductase detection in hypoxic cells and zebrafish
Results in Chemistry, 2021 Nitroreductase (NTR) is a type of cytoplasmic enzyme that depends on flavin mononucleotide or flavin adenine dinucleotide, and that is widely present in tumor cells where it increases during hypoxia. NTR can effectively reduce aromatic nitro compounds to
Wan Sun +7 more
doaj +1 more source
Enhanced small green fluorescent proteins as a multisensing platform for biosensor development
Frontiers in Bioengineering and Biotechnology, 2022 Engineered light, oxygen, and voltage (LOV)-based proteins are able to fluoresce without oxygen requirement due to the autocatalytic incorporation of exogenous flavin as a chromophore thus allowing for live cell imaging under hypoxic and anaerobic ...
Guo-Teng Liang +25 more
doaj +1 more source
Fixing flavins: hijacking a flavin transferase for equipping flavoproteins with a covalent flavin cofactor [PDF]
, 2023 Most flavin-dependent enzymes contain a dissociable flavin cofactor. We present a new approach for installing a covalent bond between a flavin cofactor and its hosting protein.
Hein, Wijma +5 more
core +1 more source
H2O2 selectively damages the binuclear iron-sulfur cluster N1b of respiratory complex I
Scientific Reports, 2023 NADH:ubiquinone oxidoreductase, respiratory complex I, plays a major role in cellular energy metabolism by coupling electron transfer with proton translocation.
Lisa Strotmann +6 more
doaj +1 more source
FMN reduces Amyloid-β toxicity in yeast by regulating redox status and cellular metabolism
Nature Communications, 2020 Saccharomyces cerevisiae is a model organism to study proteins involved in neurodegeneration. Here, the authors performed a yeast genome-wide synthetic genetic interaction array (SGA) to screen for toxicity modifiers of Aβ42 and identify riboflavin ...
Xin Chen +6 more
doaj +1 more source
Non-statistical fragmentation in photo-activated flavin mononucleotide anions [PDF]
, 2021 The spectroscopy and photo-induced dissociation of flavin mononucleotide anions in vacuo are investigated over the 300-500 nm wavelength range. Comparison of the dependence of fragment ion yields as a function of deposited photon energy with calculated ...
Bull, James N. +13 more
core +1 more source
Cysteine Thioaldehydes: Photolytic Generation, Reactivity, and Biological Implications
Angewandte Chemie, EarlyView.Photolysis of cysteine phenacylsulfides bearing non‐conjugating electron‐withdrawing substituents leads to high conversions into cysteine thioaldehydes through a Norrish type‐II pathway. This methodology enabled the study of the aqueous reactivity of these important biosynthetic intermediates, which, depending on peptide sequence, pH and buffer ...
Ardra Karthika +9 more
wiley +2 more sources
Mass spectrometry locates local and allosteric conformational changes that occur on cofactor binding
Nature Communications, 2016 The decarboxylate enzyme Fdc1 requires a prenylated flavin mononucleotide co-factor for activity. Here, the authors use a variety of mass spectrometric techniques to observe the structural changes in this protein in response to co-factor binding.
Rebecca Beveridge +5 more
doaj +1 more source
Co-Overexpression of RIB1 and RIB6 Increases Riboflavin Production in the Yeast Candida famata
Fermentation, 2022 Riboflavin or vitamin B2 is a water-soluble vitamin and a precursor of flavin coenzymes, flavin mononucleotide, and flavin adenine dinucleotide, which play a key role as enzyme cofactors in energy metabolism.
Yana Petrovska +4 more
doaj +1 more source