Results 101 to 110 of about 44,095 (208)

An analytical study on the reduction of neotetrazolium chloride by the terminal electron transport system [PDF]

, 1958
In order to determine the steps with which the reaction of neotetrazolium chloride reduction conjugates in the terminal electron transport system, an analytical study on the neotetrazolium reduction by tissue homogenates was carried out using various ...
Oda, Takuzo, Okazaki, Hiroaki
core   +1 more source

Stress Conditioned Early‐Stage Screening in Biocatalysis

ChemCatChem, Volume 18, Issue 3, 12 February 2026.
Standard enzyme screening often overlooks industrial stress. We propose integrating conditions like pH shifts, low oxygen, interfacial deactivation, and high substrate/product levels into early high‐throughput assays. By measuring stress‐relevant metrics such as turnover under stress, cofactor efficiency, and solvent stability, the enzyme selection ...
Laia Vila‐Vidal, John M. Woodley, Elif Erdem   +2 more
wiley   +1 more source

Identification of stable normalization genes for quantitative real-time PCR in porcine articular cartilage

Journal of Animal Science and Biotechnology, 2012
Background Expression levels for genes of interest must be normalized with an appropriate reference, or housekeeping gene, to make accurate comparisons of quantitative real-time PCR results.
McCulloch Ryan S, Ashwell Melissa S, O’Nan Audrey T, Mente Peter L   +3 more
doaj   +1 more source

Electrochemical Characterization of Escherichia coli Adaptive Response Protein AidB [PDF]

, 2012
When exposed to known DNA-damaging alkylating agents, Escherichia coli cells increase production of four DNA repair enzymes: Ada, AlkA, AlkB, and AidB. The role of three enzymes (Ada, AlkA, and AlkB) in repairing DNA lesions has been well characterized ...
Becker, Bowles, Byron, Catherine Drennan, Chohan, Cintyu Wong, Clark, Delaney, Demple, Evensen, Falnes, Falnes, Fink, Frerman, Frick, Ghisla, Hamill, He, Husain, Johnson, Karran, Kim, Landini, Landini, Lenn, Leys, Maier, Marco Jost, Massey, Massey, Massey, Michael Hamill, Mulrooney, Nicholas Bene, Pace, Rippa, Roberts, Roberts, Rohankhedkar, Saenger, Samson, Sean Elliott, Sedgwick, Sedgwick, Sedgwick, Stankovich, Stiehler, Swanson, Toogood, Toogood, Trewick, Vetter, Volkert, Volkert   +53 more
core   +2 more sources

Isolation of high quality RNA from soil-grown Ilex paraguariensis roots suitable for next-generation sequencing and gene expression analyses [PDF]

, 2017
Extraction of high quality RNA is a prerequisite for downstream application in functional genomics analyses. However, the extraction and purification of pure nucleic acids from root tissues is generally difficult due to the high concentration of ...
Acevedo, Raúl Maximiliano, Avico, Edgardo Hernán, Calzadilla, Pablo Ignacio, Ruiz, Oscar Adolfo, Sansberro, Pedro Alfonso   +4 more
core   +1 more source

Exploring the Potential and Evaluating Hydrocarbon Degradation by Novel Antarctic Dietzia and Pusillimonas Isolates From a Pristine Environment

Environmental Microbiology Reports, Volume 18, Issue 1, February 2026.
The Antarctic Dietzia and Pusillimonas isolates were tested for bioremediation applications. Genomic analyses revealed the potential to degrade complex organic compounds, potential virulence factors and resistance genes. Physiological assays showed a wide range of adaptation to abiotic factors and sensitivity to different groups of antibiotics ...
Tomasz Krucoń, Julia Karbowska, Wiktoria Pietrowicz, Robert Stasiuk, Łukasz Drewniak   +4 more
wiley   +1 more source

Photoinduced monooxygenation involving NAD(P)H-FAD sequential single-electron transfer

Nature Communications, 2020
The number of usable light-responsive enzymes is limited, despite the potential biotechnological applications. Here, the authors report a flavoprotein monooxygenase which is controllable by blue light illumination, and propose a mechanism involving ...
Simon Ernst, Stefano Rovida, Andrea Mattevi, Susanne Fetzner, Steffen L. Drees   +4 more
doaj   +1 more source

Crystallization and preliminary crystallographic data of the PAS domain of the NifL protein from Azotobacter vinelandii. [PDF]

, 2001
The Azotobacter vinelandii NifL protein is a redox-sensing flavoprotein which inhibits the activity of the nitrogen-specific transcriptional activator NifA.
Enroth, C., Hefti, M.H., Hendle, J., Tucker, P.A., Vervoort, J.J.M.   +4 more
core   +1 more source

Life on Mars? The physiological perspective

Experimental Physiology, EarlyView.
Ronan M. G. Berg, Damian M. Bailey
wiley   +1 more source

Mechanisms underlying metabolic and neural defects in zebrafish and human multiple acyl-CoA dehydrogenase deficiency (MADD).

PLoS ONE, 2009
In humans, mutations in electron transfer flavoprotein (ETF) or electron transfer flavoprotein dehydrogenase (ETFDH) lead to MADD/glutaric aciduria type II, an autosomal recessively inherited disorder characterized by a broad spectrum of devastating ...
Yuanquan Song, Mary A Selak, Corey T Watson, Christopher Coutts, Paul C Scherer, Jessica A Panzer, Sarah Gibbs, Marion O Scott, Gregory Willer, Ronald G Gregg, Declan W Ali, Michael J Bennett, Rita J Balice-Gordon   +12 more
doaj   +1 more source