Results 51 to 60 of about 72,796 (265)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Characterisation of molecular motions in cryo-EM single-particle data by multi-body refinement in RELION

eLife, 2018
Macromolecular complexes that exhibit continuous forms of structural flexibility pose a challenge for many existing tools in cryo-EM single-particle analysis. We describe a new tool, called multi-body refinement, which models flexible complexes as a user-
Takanori Nakane, Dari Kimanius, Erik Lindahl, Sjors HW Scheres   +3 more
doaj   +1 more source

Equilibrium internal fractures in elastic bodies supported by thin flexible coatings

Вестник Дагестанского государственного технического университета: Технические науки, 2020
Objective. In this paper, the authors study problems of a plane strain of elastic bodies containing internal rectilinear fractures. In each case, the margins of the considered areas are supported by thin flexible coatings.
B. V. Sobol, E. V. Rashidova, P. V. Vasiliev, A. I. Novikova   +3 more
doaj   +1 more source

Dynamic simulation of non-penetrating flexible bodies [PDF]

Proceedings of the 19th annual conference on Computer graphics and interactive techniques, 1992
A model for the dynamic simulation of flexible bodies subject to non-penetration constraints is presented. Flexible bodies are described in terms of global deformations of a rest shape. The dynamical behavior of these bodies that most closely matches the behavior of ideal continuum bodies is derived, and subsumes the results of earl ier Lagrangian ...
David Baraff, Andrew P. Witkin
openaire   +1 more source

The skills required for transition to university and study in biological sciences: A student perspective

FEBS Open Bio, EarlyView.
Bioscience students were asked for their opinions on the value and teaching of skills. 204 responded that teamwork, time management and study skills are necessary to reach University, that scientific writing, research, laboratory and presentation skills are taught effectively during their studies, while other skills are gained inherently through study ...
Janella Borrell, Susan Crennell
wiley   +1 more source

MBD analysis of flexible multibody system with mass loss

Nihon Kikai Gakkai ronbunshu, 2016
In this paper, we propose a formulation method for flexible multi-body systems with mass loss. The method derives the equation of motion using the velocity transformation method, in consideration of the shift of center-of-mass.
Kanjuro MAKIHARA, Takashi KIKKAWA, Yoshiki SUGAWARA   +2 more
doaj   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Energy indicators of working bodies for tillage of steam fields in summer [PDF]

BIO Web of Conferences
Currently, one of the most important stages in obtaining high yields of the main crops is tillage of steam fields in summer. The article considers various variants of working bodies for tillage of steam fields to establish the energy indicators of the ...
Kambulov Sergey, Rykov Viktor, Bozhko Igor, Podlesny Dmitry, Pakhomov Victor   +4 more
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source