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Imaging Flow Cytometry: Development, Present Applications, and Future Challenges [PDF]

open access: goldMethods and Protocols
Imaging flow cytometry (ImFC) represents a significant technological advancement in the field of cytometry, effectively merging the high-throughput capabilities of flow analysis with the detailed imaging characteristics of microscopy.
Savvas Dimitriadis   +5 more
doaj   +2 more sources

An Early Myelosuppression in the Acute Mouse Sepsis Is Partly Outcome-Dependent

open access: yesFrontiers in Immunology, 2021
Adult hematopoietic stem and progenitor cells (HSPCs) respond to bacterial infections by expansion to myeloid cells. Sepsis impairs this process by suppressing differentiation of stem cells subsequently contributing to an ineffective immune response ...
Tomasz Skirecki   +6 more
doaj   +1 more source

A gain and dynamic range independent index to quantify spillover spread to aid panel design in flow cytometry

open access: yesScientific Reports, 2021
In conventional flowcytometry one detector (primary) is dedicated for one fluorochrome. However, photons usually end up in other detectors too (fluorescence spillover).
Debajit Bhowmick   +4 more
doaj   +1 more source

Full spectrum flow cytometry and mass cytometry: A 32‐marker panel comparison

open access: yesCytometry Part A, 2022
High‐dimensional single‐cell data has become an important tool in unraveling the complexity of the immune system and its involvement in homeostasis and a large array of pathologies.
M. Jaimes   +5 more
semanticscholar   +1 more source

MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

open access: yesJournal of Extracellular Vesicles, 2020
Extracellular vesicles (EVs) are small, heterogeneous and difficult to measure. Flow cytometry (FC) is a key technology for the measurement of individual particles, but its application to the analysis of EVs and other submicron particles has presented ...
J. Welsh   +26 more
semanticscholar   +1 more source

Flow Cytometry: A Blessing and a Curse

open access: yesBiomedicines, 2021
Flow cytometry is a laser-based technology generating a scattered and a fluorescent light signal that enables rapid analysis of the size and granularity of a particle or single cell.
H. Drescher   +2 more
semanticscholar   +1 more source

Detection of Central Nervous System Infiltration by Myeloid and Lymphoid Hematologic Neoplasms Using Flow Cytometry Analysis: Diagnostic Accuracy Study

open access: yesFrontiers in Medicine, 2018
IntroductionInfiltration of the central nervous system (CNS) by hematologic or lymphoid malignant cells can cause extensive neurological damage, be progressive and fatal.
Laiz Cameirão Bento   +13 more
doaj   +1 more source

Spectral Flow Cytometry [PDF]

open access: yesCurrent Protocols in Cytometry, 2013
AbstractInterest in measuring the complete fluorescence spectra of individual cells in flow can be traced to the earliest days of flow cytometry. Recent advances in detectors, optics, and computation have made it possible to make full spectral measurements in the sub‐millisecond time frame in which flow cytometry measurements typically occur.
John P, Nolan, Danilo, Condello
openaire   +2 more sources

ROR1 Flow Cytometry [PDF]

open access: yesBIO-PROTOCOL, 2013
ROR1 is a receptor tyrosine kinase family member studied for its roles in development and cancer. Here we describe a protocol for analysis of ROR1 surface expression in acute lymphoblastic leukemia immortalized cell lines by flow cytometry.
Vincent Bicocca, Jeffrey Tyner
openaire   +3 more sources

OMIP‐069: Forty‐Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood

open access: yesCytometry Part A, 2020
This 40‐color flow cytometry‐based panel was developed for in‐depth immunophenotyping of the major cell subsets present in human peripheral blood. Sample availability can often be limited, especially in cases of clinical trial material, when multiple ...
Lily M. Park, J. Lannigan, M. Jaimes
semanticscholar   +1 more source

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