Results 11 to 20 of about 1,011,693 (349)

Unravelling Plasma Extracellular Vesicle Diversity With Optimised Spectral Flow Cytometry [PDF]

Journal of Extracellular Biology
Extracellular vesicles (EVs) are crucial for intercellular communication and are found in various biological fluids. The identification and immunophenotyping of such small particles continue to pose significant challenges.
Daniela Boselli, Francesca Clemente, Simona Di Terlizzi, Christina Pagiatakis, Laura Papa, Genny Del Zotto, Chiara Villa, Giuseppe Alvise Ramirez, Norma Maugeri, Angelo A. Manfredi, Achille Anselmo   +10 more
doaj   +2 more sources

Comparison of methods for in-house screening of HLA*B57:01 to prevent abacavir hypersensitivity in HIV-1 care [PDF]

, 2015
Abacavir is a nucleoside reverse transcriptase inhibitor used as part of combination antiretroviral therapy in HIV-1-infected patients. Because this drug can cause a hypersensitivity reaction that is correlated with the presence of the HLA-B*57:01 ...
Bonczkowski, Pawel, Callens, Steven, Coucke, Paul, De Spiegelaere, Ward, De Wit, Stephane, Emonds, Marie-Paule, Kabeya, Kabamba, Kiselinova, Maja, Malatinková, Eva, Philippé, Jan, Ruelle, Jean, SERMIJN, ERICA, Trypsteen, Wim, Van Acker, Petra, Van Sandt, Vicky, Vandekerckhove, Linos, Verhofstede, Chris, VERVISCH, KAREN, Vogelaers, Dirk   +18 more
core   +14 more sources

The in vitro micronucleus assay using imaging flow cytometry and deep learning

npj Systems Biology and Applications, 2021
The in vitro micronucleus (MN) assay is a well-established assay for quantification of DNA damage, and is required by regulatory bodies worldwide to screen chemicals for genetic toxicity.
Matthew A. Rodrigues, Christine E. Probst, Artiom Zayats, Bryan Davidson, Michael Riedel, Yang Li, Vidya Venkatachalam   +6 more
doaj   +1 more source

Rapid evaluation of T cell clonality in the diagnostic work-up of mature T cell neoplasms: TRBC1-based flow cytometric assay experience

Translational Oncology, 2022
The identification of mature T cell neoplasms by flow cytometry is often challenging, due to overlapping features with reactive T cells and limitations of currently available T cell clonality assays. The description of an antibody specific for one of two
Manuela Capone, Benedetta Peruzzi, Boaz Palterer, Sara Bencini, Alessandro Sanna, Benedetta Puccini, Luca Nassi, Benedetta Salvadori, Marinella Statello, Alessia Carraresi, Stefania Stefanelli, Chiara Orazzini, Barbara Minuti, Roberto Caporale, Francesco Annunziato   +14 more
doaj   +1 more source

Multi-angle pulse shape detection of scattered light in flow cytometry for label-free cell cycle classification

Communications Biology, 2021
Combining multi-angle (MA) light scatter detection with time-resolved pulse shape (PS) analysis for flow cytometry (FC), Kage et al. report MAPS-FC method for cell cycle analysis.
Daniel Kage, Kerstin Heinrich, Konrad v. Volkmann, Jenny Kirsch, Kristen Feher, Claudia Giesecke-Thiel, Toralf Kaiser   +6 more
doaj   +1 more source

An Early Myelosuppression in the Acute Mouse Sepsis Is Partly Outcome-Dependent

Frontiers in Immunology, 2021
Adult hematopoietic stem and progenitor cells (HSPCs) respond to bacterial infections by expansion to myeloid cells. Sepsis impairs this process by suppressing differentiation of stem cells subsequently contributing to an ineffective immune response ...
Tomasz Skirecki, Susanne Drechsler, Aldona Jeznach, Grażyna Hoser, Mohammad Jafarmadar, Jerzy Kawiak, Marcin F. Osuchowski   +6 more
doaj   +1 more source

A UV‐C LED‐based unit for continuous decontamination of the sheath fluid in a flow‐cytometric cell sorter

Engineering in Life Sciences, 2022
Aseptic cell sorting is challenging, especially when a flow‐cytometric cell sorter is not operated in a sterile environment. The sheath fluid system of a cell sorter may be contaminated with germs such as bacteria, yeasts, viruses, or fungi.
Jenny Kirsch, Kerstin Heinrich, Daniel Kage, Johannes Glaab, Benjamin Grothe, Konrad v. Volkmann, Toralf Kaiser   +6 more
doaj   +1 more source

A gain and dynamic range independent index to quantify spillover spread to aid panel design in flow cytometry

Scientific Reports, 2021
In conventional flowcytometry one detector (primary) is dedicated for one fluorochrome. However, photons usually end up in other detectors too (fluorescence spillover).
Debajit Bhowmick, Frank van Diepen, Anita Pfauth, Renaud Tissier, Michelle L. Ratliff   +4 more
doaj   +1 more source

Deep Cytometry: Deep learning with Real-time Inference in Cell Sorting and Flow Cytometry [PDF]

, 2019
Deep learning has achieved spectacular performance in image and speech recognition and synthesis. It outperforms other machine learning algorithms in problems where large amounts of data are available.
Chen, Claire Lifan, Jalali, Bahram, Li, Yueqin, Mahjoubfar, Ata, Niazi, Kayvan Reza, Pei, Li   +5 more
core   +2 more sources

Phenotyping renal leukocyte subsets by four-color flow cytometry: Characterization of chemokine receptor expression [PDF]

, 2003
To investigate mechanisms of cell-mediated injury in renal inflammatory disease it is critical to determine the surface phenotype of infiltrating renal leukocyte subsets.
Anders, Hans-Joachim, de Lema, Guillermo Pérez, Luckow, Bruno, Mack, Matthias, Schlöndorff, Detlef, Vielhauer, Volker   +5 more
core   +1 more source