Results 41 to 50 of about 1,011,693 (349)

Cell sorting based on pulse shapes from angle resolved detection of scattered light

Communications Biology
Flow cytometry is a key technology for the analysis and sorting of cells or particles at high throughput. Conventional and current flow cytometry is primarily based on fluorescent stains to detect the cells of interest.
Daniel Kage, Andrej Eirich, Kerstin Heinrich, Jenny Kirsch, Jan Popien, Alexander Wolf, Konrad v. Volkmann, Hyun-Dong Chang, Toralf Kaiser   +8 more
doaj   +1 more source

Human Neutrophil Antigen Genotype and Allele Frequencies in Iranian Blood Donors

Journal of Immunology Research, 2022
Objective. Human neutrophil antigens (HNAs) can be targeted by HNA-allo antibodies and cause a variety of clinical conditions such as transfusion-related acute lung injury (TRALI) and neonatal alloimmune neutropenia (NAIN).
Behnaz Esmaeili, Behnaz Bayat, Atefe Alirezaee, Mona Delkhah, Mohammad Reza Mehdizadeh, Zahra Pourpak   +5 more
doaj   +1 more source

B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses

FEBS Letters, EarlyView.
B cells sense external mechanical forces and convert them into biochemical signals through mechanotransduction. Understanding how malignant B cells respond to physical stimuli represents a groundbreaking area of research. This review examines the key mechano‐related molecules and pathways in B lymphocytes, highlights the most relevant techniques to ...
Marta Sampietro, Marco Cellani, Cristina Scielzo   +2 more
wiley   +1 more source

Harmonization of Flow Cytometric Minimal Residual Disease Assessment in Multiple Myeloma in Centers of Polish Myeloma Consortium

Diagnostics, 2021
Minimal residual disease (MRD) status is now considered as one of the most relevant prognostic factors in multiple myeloma (MM) while MRD negativity became an important endpoint in clinical trials.
Agnieszka Krzywdzińska, Bartosz Puła, Anna Czyż, Beata Krzymieniewska, Jolanta Kiernicka-Parulska, Anna Mierzwa, Donata Szymczak, Aneta Milanowska, Aleksandra Kiraga, Iwona Kwiecień, Joanna Zaleska, Krzysztof Jamroziak   +11 more
doaj   +1 more source

Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutation

FEBS Letters, EarlyView.
This study reveals how prime editing guide RNA (pegRNA) secondary structure and reverse transcriptase template length affect prime editing efficiency in correcting the phospholamban R14del cardiomyopathy‐associated mutation. Insights support the design of structurally optimized enhanced pegRNAs for precise gene therapy.
Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei   +7 more
wiley   +1 more source

HPLC and flow cytometry combined approach for HbF analysis in fetomaternal haemorrhage evaluation

Practical Laboratory Medicine
Introduction: Recently, a flow cytometric (FC) based test has been developed for detection of circulating fetal cells to replace the less accurate and reproducible Kleihauer-Betke test.FC test is easier to perform, it can distinguish the origin of fetal ...
Benedetta Peruzzi, Serena Guerrieri, Tiziana Biagioli, Luisa Lanzilao, Sara Pratesi, Sara Bencini, Marinella Statello, Alessia Carraresi, Stefania Stefanelli, Martina Tonelli, Marco Brogi, Manuela Capone, Alessio Mazzoni, Anna Maria Grazia Gelli, Alessandra Fanelli, Roberto Caporale, Francesco Annunziato   +16 more
doaj   +1 more source

Single‐cell insights into the role of T cells in B‐cell malignancies

FEBS Letters, EarlyView.
Single‐cell technologies have transformed our understanding of T cell–tumor cell interactions in B‐cell malignancies, revealing new T‐cell subsets, functional states, and immune evasion mechanisms. This Review synthesizes these findings, highlighting the roles of T cells in pathogenesis, progression, and therapy response, and underscoring their ...
Laura Llaó‐Cid
wiley   +1 more source

The utility of flow cytometry for the detection of tumor cells in cerebrospinal fluid of patients with acute leukemia

Universitas Scientiarum, 2022
Central nervous system infiltration by acute leukemia is a poor prognosis variable, and conventional cytology is the gold standard for its diagnosis; the technique is highly specific but not sensitive.
Ximena Torres, Iliana De Los Reyes, Martha Vizcaíno, Paula Carolina Guzmán, Alba Campos, Niyireth Peñalosa, Ana María Uribe, Carlos Saavedra, Martha Romero, Gina Cuéllar, Liliana Martin, Paula Rodriguez, Claudia Cardozo, Sandra Quijano   +13 more
doaj   +1 more source

A multi-color fast-switching microfluidic droplet dye laser [PDF]

, 2009
We describe a multi-color microfluidic dye laser operating in whispering gallery mode based on a train of alternating droplets containing solutions of different dyes; this laser is capable of switching the wavelength of its emission between 580 nm and ...
Abate, Adam R., Agresti, Jeremy J., Li, Zhenyu, Psaltis, Demetri, Tang, Sindy K. Y., Weitz, David A., Whitesides, George M.   +6 more
core   +1 more source

Cytogenomic features of Richter transformation

Molecular Cytogenetics, 2023
Background Richter transformation (RT) is the development of aggressive lymphoma in patients with chronic lymphocytic leukemia (CLL) or small lymphocytic lymphoma (SLL). This rare disease is characterised by dismal prognosis.
Renata Woroniecka, Grzegorz Rymkiewicz, Zbigniew Bystydzienski, Barbara Pienkowska-Grela, Jolanta Rygier, Natalia Malawska, Katarzyna Wojtkowska, Nikolina Goral, Katarzyna Blachnio, Marcin Chmielewski, Magdalena Bartnik-Glaska, Beata Grygalewicz   +11 more
doaj   +1 more source